Difference between revisions of "Team:TCU Taiwan/Description"

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                <br>AMP. <I>coli</I>
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<h2> Project Description </h2>
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To achieve our goal we incorporated antimicrobial peptides (AMPs) into our medical dressing. AMPs, are stable peptide that have extensive ability in bactericidal effects. Unlike antibiotics, AMPs can puncture the cell membrane to kill the bacteria therefore bypassing bacterial antibiotic drug resistance mechanisms.  <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Overview#tcu_references_1">[1]</a> Besides, the peptides also have ability to help skin recovered.  <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Overview#tcu_references_2"> [2]</a>After reading numerous of research articles, we selected two kinds of AMPs: Signiferin and Epinecidin-1 as our reagents.<br><br>  
  
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
 
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<h5>What should this page contain?</h5>
 
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<li> A clear and concise description of your project.</li>
 
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
 
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<li>References and sources to document your research.</li>
 
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<li>Use illustrations and other visual resources to explain your project.</li>
 
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Signiferin is a peptide came from the skin mucus of <I>Crinia signifera</I>. It demonstrated effectiveness in killing Methicillin-Resistant <I>Staphylococcus aureus</I> (MRSA), and has already been demonstrated by the TU-Delft 2013 iGEM team.<a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Overview#tcu_references_3">[3]</a>Epinecidin-1 is a peptide came from the skin mucus of <I>Epinephelus coioides</I>. It has ability to help wound healing and has been proven by animal studies, and was selected as an additional reagent. <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Overview#tcu_references_4">[4]</a> By combining these two properties, we believe that can develop a wound dressing that may be useful in trauma patients without the additional risk of developing drug-resistance.<br><br>
  
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<h4>Advice on writing your Project Description</h4>
 
  
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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To control the AMPs expression and secretion, the <I>Lac</I> operon was used and treated signal peptide into our system. Helping peptides secret into culture medium. <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Overview#tcu_references_5">[5][6]</a> After purification of the peptide we will be testing the effectiveness of our synthetic AMPs. We will test macro-dilution of MRSA and in vitro wound healing assay for epithelial cells line (HaCaT) and micro vascular endothelial cells (HMEC-1). Next, application to the mouse model was conducted in vivo. Out goal is to create a wound dressing that is effective in inhibiting bacterial growth and assisting wound healing process.
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    <a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Our_Design">
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            &nbsp;&nbsp;Antimicrobial peptide
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<p align="justify">&bull;&nbsp;Epinecidin-1:</p>
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        <td style="vertical-align:text-top;">1.</td>
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        <td wigth="80%">From the skin mucus of <I>Epinephelus coioides</I> a kind of fish.</td>
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        <td style="vertical-align:text-top;">2.</td>
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        <td wigth="80%">Has function of killing bacteria.</td>
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        <td style="vertical-align:text-top;">3.</td>
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        <td wigth="80%">In addition, it has the ability to help wounds healing and has been proven by animal studies.</td>
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<p align="justify">&bull;&nbsp; Signiferin:
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        <td style="vertical-align:text-top;">1.</td>
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        <td wigth="80%">From the skin mucus of <I>Crinia signifera</I> a kind of tree frog.</td>
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        <td style="vertical-align:text-top;">2.</td>
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        <td wigth="80%">Have function of killing bacteria.</td>
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        <td style="vertical-align:text-top;">3.</td>
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        <td wigth="80%">Have great ability in disinfect Methicillin-Resistant <I>Staphylococcus aureus</I> (MRSA).</td>
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        <td style="vertical-align:text-top;">4.</td>
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        <td wigth="80%">Had already been kindly proved by the 2013 TU-Delft iGEM team.</td>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Experimental">Signal peptide: </a>
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        <td style="vertical-align:text-top;">1.</td>
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        <td wigth="80%">Helps AMPs to secret out of E. <I>coli</I>.</td>
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        <td style="vertical-align:text-top;">2.</td>
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        <td wigth="80%">From <I>Streptomyces lividans</I> to trasport chitinase C to secretion system, which has been proven to work in E.<I>coli</I>  by reference.</td>
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<a href="https://2015.igem.org/Team:TCU_Taiwan/Project/Reference">Wound dressing:</a>
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  <p align="justify">
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<h4>References</h4>
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Based on AMPs to develop into a potential material of wound dressing.
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
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<h4>Inspiration</h4>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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<td style="vertical-align:text-top;" align="left">[1]</td>
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Lai Y, Gallo RL. AMPed up immunity: how antimicrobial peptides have multiple roles  in immune defense. Trends Immunol. 2009 Mar; 30(3):131-41. doi: 10.1016/j.it.2008.12.003. Epub 2009 Feb 13.
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<td style="vertical-align:text-top;" align="left">[2]</td>
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Huang HN, Rajanbabu V, Pan CY, Chan YL, Wu CJ, Chen JY. Use of the antimicrobial peptide Epinecidin-1 to protect against MRSA infection in mice with skin injuries. Biomaterials. 2013 Dec; 34(38):10319-27. doi: 10.1016/j.biomaterials.2013.09.037. Epub 2013 Sep 27.
  
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<td style="vertical-align:text-top;" align="left">[3]</td>
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Maselli VM, Bilusich D, Bowie JH, Tyler MJ. Host-defence skin peptides of the Australian Streambank Froglet Crinia riparia: isolation and sequence determination by positive and negative ion electrospray mass spectrometry. Rapid Commun Mass Spectrom. 2006; 20(5):797-803.
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<a name="tcu_references_5"></a>
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<td style="vertical-align:text-top;" align="left">[4]</td>
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Tokuyasu K, Kaneko S, Hayashi K, Mori Y. Production of a recombinant chitin deacetylase in the culture medium of Escherichia coli cells. FEBS Lett. 1999 Sep 10; 458(1):23-6.
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<td style="vertical-align:text-top;" align="left">[5]</td>
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Fujii T, Miyashita K. Multiple domain structure in a chitinase gene (chiC) of Streptomyces lividans. J Gen Microbiol. 1993 Apr; 139(4):677-86.
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Revision as of 05:32, 4 September 2015



AMP. coli

To achieve our goal we incorporated antimicrobial peptides (AMPs) into our medical dressing. AMPs, are stable peptide that have extensive ability in bactericidal effects. Unlike antibiotics, AMPs can puncture the cell membrane to kill the bacteria therefore bypassing bacterial antibiotic drug resistance mechanisms. [1] Besides, the peptides also have ability to help skin recovered. [2]After reading numerous of research articles, we selected two kinds of AMPs: Signiferin and Epinecidin-1 as our reagents.

Signiferin is a peptide came from the skin mucus of Crinia signifera. It demonstrated effectiveness in killing Methicillin-Resistant Staphylococcus aureus (MRSA), and has already been demonstrated by the TU-Delft 2013 iGEM team.[3]Epinecidin-1 is a peptide came from the skin mucus of Epinephelus coioides. It has ability to help wound healing and has been proven by animal studies, and was selected as an additional reagent. [4] By combining these two properties, we believe that can develop a wound dressing that may be useful in trauma patients without the additional risk of developing drug-resistance.

To control the AMPs expression and secretion, the Lac operon was used and treated signal peptide into our system. Helping peptides secret into culture medium. [5][6] After purification of the peptide we will be testing the effectiveness of our synthetic AMPs. We will test macro-dilution of MRSA and in vitro wound healing assay for epithelial cells line (HaCaT) and micro vascular endothelial cells (HMEC-1). Next, application to the mouse model was conducted in vivo. Out goal is to create a wound dressing that is effective in inhibiting bacterial growth and assisting wound healing process.

  Antimicrobial peptide

• Epinecidin-1:

1. From the skin mucus of Epinephelus coioides a kind of fish.
2. Has function of killing bacteria.
3. In addition, it has the ability to help wounds healing and has been proven by animal studies.

•  Signiferin:

1. From the skin mucus of Crinia signifera a kind of tree frog.
2. Have function of killing bacteria.
3. Have great ability in disinfect Methicillin-Resistant Staphylococcus aureus (MRSA).
4. Had already been kindly proved by the 2013 TU-Delft iGEM team.

Signal peptide:

1. Helps AMPs to secret out of E. coli.
2. From Streptomyces lividans to trasport chitinase C to secretion system, which has been proven to work in E.coli by reference.

Wound dressing:

Based on AMPs to develop into a potential material of wound dressing.

References


[1] Lai Y, Gallo RL. AMPed up immunity: how antimicrobial peptides have multiple roles in immune defense. Trends Immunol. 2009 Mar; 30(3):131-41. doi: 10.1016/j.it.2008.12.003. Epub 2009 Feb 13.

[2] Huang HN, Rajanbabu V, Pan CY, Chan YL, Wu CJ, Chen JY. Use of the antimicrobial peptide Epinecidin-1 to protect against MRSA infection in mice with skin injuries. Biomaterials. 2013 Dec; 34(38):10319-27. doi: 10.1016/j.biomaterials.2013.09.037. Epub 2013 Sep 27.

[3] Maselli VM, Bilusich D, Bowie JH, Tyler MJ. Host-defence skin peptides of the Australian Streambank Froglet Crinia riparia: isolation and sequence determination by positive and negative ion electrospray mass spectrometry. Rapid Commun Mass Spectrom. 2006; 20(5):797-803.

[4] Tokuyasu K, Kaneko S, Hayashi K, Mori Y. Production of a recombinant chitin deacetylase in the culture medium of Escherichia coli cells. FEBS Lett. 1999 Sep 10; 458(1):23-6.

[5] Fujii T, Miyashita K. Multiple domain structure in a chitinase gene (chiC) of Streptomyces lividans. J Gen Microbiol. 1993 Apr; 139(4):677-86.








             
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