Difference between revisions of "Team:Aalto-Helsinki/Safety"

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<h2>Safety in iGEM</h2>
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<h2>Safety in iGEM Project</h2>
  
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<h3>Safe Lab Work</h3>
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<p></br>We worked at Level 1 (low risk) laboratory because the only bacteria we handled were the strains TOP10 and BL21 (DE3) of <i>Escherichia coli</i>. The enzymes of our bacteria were foreign expressing genes from the bacterial species of <i>Clostridium</i>, <i>Haemophilus</i>, <i>Mycobacterium</i>, <i>Prochlorococcus</i> and <i>Bacillus</i> families but as the enzymes only produced intermediates, no real pathogen risks were caused. We ordered the synthesis of these genes which weren’t available as BioBricks. The companies are constantly blasting the ordered gene sequences with the pathogenic ones so the shipping isn’t preceded if hazardous material is found.</br></br>
  
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Everyone in our team working in the lab has previously worked in the lab and received mandatory training on how to work in safely in the laboratory as a part of their studies. Some of the topics of this training include personal protective equipment, how to work with different chemicals and GMO and dispose of them, how to mark reagents, on safety labels, on biosafety levels, on most common lab accidents and how to avoid them, what to do in case of an accident, on different types of hoods (laminar flow cabinet, fume hood), on the importance of proper planning and cleaning up after oneself, etc.</br></br>
  
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<h4>Safe Project Design</h4>
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Additionally, we had for our team members working in the lab a lecture on lab safety held by an instructor from the lab we are working at, covering similar topics (PPE, waste disposal etc.). Furthermore, the location of first aid kits, fire extinguishers and emergency showers in the laboratory were shown to us by an instructor. None of members are allowed to be alone in the lab and working during nights or weekend needed always permission from the lab personnel.</br></br>
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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Our strain will produce highly flammable propane gas which lower flammable limit is 1,8 % and upper 8,4 % of air volume (Airgas, 2014). In the study Turku University (Kallio et al. 2014) made the maximum produced propane amount without extra 80% oxygen addition was 0,5 mg/L which can be considered to be below flammable concentration. Still, the accumulation of propane was prevented with proper ventilation during the cultivation processes for GC-analysis and continuous production. MSDS must be read before handling the strain producing propane.
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</p>
  
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<ul>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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</ul>
 
  
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<h4>Safe Lab Work</h4>
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<h3>Safe Project Design</h3>
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<p></br>These questions were considered when evaluating the safety of our application:</br></br>
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<b>Who will use your product? What opinions do these people have about your project?</b></br></br>
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Consumers who will use propane as a fuel for vehicles and cooking. Most likely they would value the sustainable fuel and increasing options for commercial biobased fuels.</br></br> 
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<b>Where will your product be used? On a farm, in a factory, inside human bodies, in the ocean?</b></br></br>
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The product will be probably used in urban areas and next to main roads.</br></br>
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<b>If your product is successful, who will receive benefits and who will be harmed?</b></br></br>
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The overall population will get benefits from decreased CO2 emissions affecting to the climate change but it would be hard to determine how significant the impact would be. However, even the biofuels have the emissions of NOx, SOx and microparticles but when comparing to other biofuels, propane gas burns cleaner.</br></br>
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<b>What happens when it's all used up? Will it be sterilized, discarded, or recycled?</b></br></br>
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Propane cylinders will be recycled or straight refilled by the services responding about distribution. Consumers won’t be allowed to handle a ventile if the container is not connected to device for the purpose. </br></br>
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<b>Is it safer, cheaper, or better than other technologies that do the same thing?</b></br></br>
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It has the benefit of better energy consumption. Storing may contain more hazards compared to non-pressurized biofuels like ethanol but as the safe infrastructure already exists, consumer can rely that the new product is safe to use.
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</p>
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
 
<h4>Safe Shipment</h4>
 
<h4>Safe Shipment</h4>

Revision as of 09:36, 4 September 2015

Safety in iGEM Project

Safe Lab Work


We worked at Level 1 (low risk) laboratory because the only bacteria we handled were the strains TOP10 and BL21 (DE3) of Escherichia coli. The enzymes of our bacteria were foreign expressing genes from the bacterial species of Clostridium, Haemophilus, Mycobacterium, Prochlorococcus and Bacillus families but as the enzymes only produced intermediates, no real pathogen risks were caused. We ordered the synthesis of these genes which weren’t available as BioBricks. The companies are constantly blasting the ordered gene sequences with the pathogenic ones so the shipping isn’t preceded if hazardous material is found.

Everyone in our team working in the lab has previously worked in the lab and received mandatory training on how to work in safely in the laboratory as a part of their studies. Some of the topics of this training include personal protective equipment, how to work with different chemicals and GMO and dispose of them, how to mark reagents, on safety labels, on biosafety levels, on most common lab accidents and how to avoid them, what to do in case of an accident, on different types of hoods (laminar flow cabinet, fume hood), on the importance of proper planning and cleaning up after oneself, etc.

Additionally, we had for our team members working in the lab a lecture on lab safety held by an instructor from the lab we are working at, covering similar topics (PPE, waste disposal etc.). Furthermore, the location of first aid kits, fire extinguishers and emergency showers in the laboratory were shown to us by an instructor. None of members are allowed to be alone in the lab and working during nights or weekend needed always permission from the lab personnel.

Our strain will produce highly flammable propane gas which lower flammable limit is 1,8 % and upper 8,4 % of air volume (Airgas, 2014). In the study Turku University (Kallio et al. 2014) made the maximum produced propane amount without extra 80% oxygen addition was 0,5 mg/L which can be considered to be below flammable concentration. Still, the accumulation of propane was prevented with proper ventilation during the cultivation processes for GC-analysis and continuous production. MSDS must be read before handling the strain producing propane.

Safe Project Design


These questions were considered when evaluating the safety of our application:

Who will use your product? What opinions do these people have about your project?

Consumers who will use propane as a fuel for vehicles and cooking. Most likely they would value the sustainable fuel and increasing options for commercial biobased fuels.

Where will your product be used? On a farm, in a factory, inside human bodies, in the ocean?

The product will be probably used in urban areas and next to main roads.

If your product is successful, who will receive benefits and who will be harmed?

The overall population will get benefits from decreased CO2 emissions affecting to the climate change but it would be hard to determine how significant the impact would be. However, even the biofuels have the emissions of NOx, SOx and microparticles but when comparing to other biofuels, propane gas burns cleaner.

What happens when it's all used up? Will it be sterilized, discarded, or recycled?

Propane cylinders will be recycled or straight refilled by the services responding about distribution. Consumers won’t be allowed to handle a ventile if the container is not connected to device for the purpose.

Is it safer, cheaper, or better than other technologies that do the same thing?

It has the benefit of better energy consumption. Storing may contain more hazards compared to non-pressurized biofuels like ethanol but as the safe infrastructure already exists, consumer can rely that the new product is safe to use.

Safe Shipment

Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?