Difference between revisions of "Team:UCLA/Notebook/Recombinant Expression/29 May 2015"
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* Plasmid ran on gel to verify size of plasmid, sequenced to verify proper insert, the will be used to transform into BL21(DE3) cells. | * Plasmid ran on gel to verify size of plasmid, sequenced to verify proper insert, the will be used to transform into BL21(DE3) cells. | ||
| + | =Julian's Work= | ||
| + | *I inoculated a 500mL culture in LB with Chlor at a 1000X dilution. I used a 5mL starter culture grown overnight also in LB. I am measuring the OD every hour or so. After the first hour it was onlt 0.02 so I will let it grow for longer before inoculating. | ||
| + | **This culture will be used to make the hydrogel assays. | ||
Latest revision as of 20:24, 29 May 2015
- Starter Culture Failure #3 : Back in Black
- Starter cultures grown on Wednesday were taken from the incubator at 1300 hours (~48 hours) and OD600 was taken on the samples.
- Tamura Plate 3 -- OD600: 1.273, Tamura Plate 4-- OD600: 1.246, Tamura Plate 5: OD600: 1.279.
- Starter cultures grown on Wednesday were taken from the incubator at 1300 hours (~48 hours) and OD600 was taken on the samples.
- Plasmid was mini prepped using the Promega PureYield Plasmid Miniprep System
- Plasmid ran on gel to verify size of plasmid, sequenced to verify proper insert, the will be used to transform into BL21(DE3) cells.
Julian's Work
- I inoculated a 500mL culture in LB with Chlor at a 1000X dilution. I used a 5mL starter culture grown overnight also in LB. I am measuring the OD every hour or so. After the first hour it was onlt 0.02 so I will let it grow for longer before inoculating.
- This culture will be used to make the hydrogel assays.