Difference between revisions of "Team:CAU China/Notebook"
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<p>PCR reaction on pER41G-A</p> | <p>PCR reaction on pER41G-A</p> | ||
<img src="https://static.igem.org/mediawiki/2015/3/37/CAU_notebook_1.JPG" width="400px"> | <img src="https://static.igem.org/mediawiki/2015/3/37/CAU_notebook_1.JPG" width="400px"> | ||
| − | + | <p>Gel purification was performed on the PCR product</P> | |
| + | <p>The product was named as Ap-PCR(P)</p> | ||
| + | <p>Mix the Ap-PCR(P) and pER41G-A </p> | ||
| + | <p>Digest the mixture with restriction enzymes: XbaⅠ-HF and SacⅠ</p> | ||
| + | <img src="CAU_notebook_2.JPG" width="400px"> | ||
</html> | </html> | ||
Revision as of 04:35, 6 September 2015
Notebook
Lab Journal
"Do not, for one repulse, forgot the purpose that you resolved to effort." - William Shakespeare
Wednesday 1/4
We started our work in the wet lab with a one-day lab safety course. We learned basic lab safety knowledge, such as how to put out a fire in the lab, how to deal with the poisonous chemical and the dangerous bacteria.
Thursday 2/4
We started to get familiar with our lab. We learned about where we can find everything that is needed for working in the lab. In addition, we had a lab meeting about the project goals and the way how to get there.
Friday 3/4
PCR reaction on pER41G-A
Gel purification was performed on the PCR product
The product was named as Ap-PCR(P)
Mix the Ap-PCR(P) and pER41G-A
Digest the mixture with restriction enzymes: XbaⅠ-HF and SacⅠ
