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| In summer, to enhance the communication between iGEM teams and share the experience of preparation, Wuhan University held the ‘ mid-china iGEM seminar’, with Huazhong University of Science and Technology and Huazhong Agricultural University. | | In summer, to enhance the communication between iGEM teams and share the experience of preparation, Wuhan University held the ‘ mid-china iGEM seminar’, with Huazhong University of Science and Technology and Huazhong Agricultural University. |
| </div> | | </div> |
− | <img src="human_practice_image/1.jpg" class = "img" width="50%"> | + | <img src="https://static.igem.org/mediawiki/2015/a/ac/WHU-China_2_1.jpg" class = "img" width="50%"> |
| <div class="para1">1. Time and space <br> | | <div class="para1">1. Time and space <br> |
| <div class="para1_con">On July 29.2015; in the College of Life science , Wuhan University.</div> | | <div class="para1_con">On July 29.2015; in the College of Life science , Wuhan University.</div> |
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| <div class= "main_content_2" id="hp2" style="display:none"> | | <div class= "main_content_2" id="hp2" style="display:none"> |
− | <img src="human_practice_image/2.jpg" width="50%"> | + | <img src="https://static.igem.org/mediawiki/2015/0/0f/WHU-China_2_2.jpg" width="50%"> |
− | <img src="human_practice_image/3.jpg" width="50%" style="margin-top:35%"> | + | <img src="https://static.igem.org/mediawiki/2015/a/a2/WHU-China_2_3.jpg" width="50%" style="margin-top:35%"> |
− | <img src="human_practice_image/4.jpg" width="50%" style="margin-top:70%"> | + | <img src="https://static.igem.org/mediawiki/2015/d/dd/WHU-China_2_4.jpg" width="50%" style="margin-top:70%"> |
| </div> | | </div> |
| <style type="text/css"> | | <style type="text/css"> |
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| <h2>3. Discussion</h2> | | <h2>3. Discussion</h2> |
| <div class="txt"> | | <div class="txt"> |
− | <img src="human_practice_image/5.jpg" width="30%" /> | + | <img src="https://static.igem.org/mediawiki/2015/d/d0/WHU-China_2_5.jpg" width="30%" /> |
| <p>During the discussion, we first shared our difficulties in the experiment and the solutions, such as the efficiency of transformation, activity of the enzymes. Then we came up with an idea that we could build a platform, where we communicate the up to date findings of synthesis biology, which can give us some clues of the match. Every team leader will take the responsibility to manage the platform in turn. And we can share some efficient technology and agentia for the success of experiments | | <p>During the discussion, we first shared our difficulties in the experiment and the solutions, such as the efficiency of transformation, activity of the enzymes. Then we came up with an idea that we could build a platform, where we communicate the up to date findings of synthesis biology, which can give us some clues of the match. Every team leader will take the responsibility to manage the platform in turn. And we can share some efficient technology and agentia for the success of experiments |
| </p> | | </p> |
| </div> | | </div> |
| <div class="txt"> | | <div class="txt"> |
− | <img src="human_practice_image/6.jpg" width="30%" /> | + | <img src="https://static.igem.org/mediawiki/2015/f/fb/WHU-China_2_6.jpg" width="30%" /> |
| <p> | | <p> |
| Secondly, we communicated about the HP. How to find a feasible theme, how to carry out, how to promote the cooperation with other teams, how to present the HP and so on. Every team shared their own methods. For example, the HUST said they cooperated with other colleges and combined it with social work. | | Secondly, we communicated about the HP. How to find a feasible theme, how to carry out, how to promote the cooperation with other teams, how to present the HP and so on. Every team shared their own methods. For example, the HUST said they cooperated with other colleges and combined it with social work. |
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| </div> | | </div> |
| <div class="txt"> | | <div class="txt"> |
− | <img src="human_practice_image/7.jpg" width="30%" /> | + | <img src="https://static.igem.org/mediawiki/2015/0/0b/WHU-China_2_7.jpg" width="30%" /> |
| <p> | | <p> |
| Finally, we discussed the build of our teams. The management, the scale, the structure of age, the responsibility distribution are all our concerns, which could give a great reference to the next team. | | Finally, we discussed the build of our teams. The management, the scale, the structure of age, the responsibility distribution are all our concerns, which could give a great reference to the next team. |
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| <b>Preparation: </b> | | <b>Preparation: </b> |
| <div class = "txt"> | | <div class = "txt"> |
− | <img src="human_practice_image/16.jpg" class="img_left" width="20%" style="margin-top:5%"> | + | <img src="https://static.igem.org/mediawiki/2015/3/38/WHU-China_2_16.jpg" class="img_left" width="20%" style="margin-top:5%"> |
| <p>1. “Colors” : GFP and RFP gene under the control of strong promoter and strong RBS on vector pSB1C3 are transformed into E. Coli separately. E.coli expressing GFP and RFP are cultured in Lysogeny-Broth medium.</p> | | <p>1. “Colors” : GFP and RFP gene under the control of strong promoter and strong RBS on vector pSB1C3 are transformed into E. Coli separately. E.coli expressing GFP and RFP are cultured in Lysogeny-Broth medium.</p> |
| <p>2. “Drawing Papers” : Disposable dishes are sterilized in an autoclave. Lysogeny-Broth medium ( solid ) with chloramphenicol is used to culture E. Coli after painting.</p> | | <p>2. “Drawing Papers” : Disposable dishes are sterilized in an autoclave. Lysogeny-Broth medium ( solid ) with chloramphenicol is used to culture E. Coli after painting.</p> |
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| <p style="margin-left:0">1. We tried to attract people to come by hanging out posters, sending invitations to primary schools nearby and etc.</p></div> | | <p style="margin-left:0">1. We tried to attract people to come by hanging out posters, sending invitations to primary schools nearby and etc.</p></div> |
| <div class = "txt"><p style="margin-left:10%">2. We taught kids and their parents how to draw a sketch: </p> | | <div class = "txt"><p style="margin-left:10%">2. We taught kids and their parents how to draw a sketch: </p> |
− | <img src="human_practice_image/11.jpg" class="img_left" width="20%"> | + | <img src="https://static.igem.org/mediawiki/2015/4/4e/WHU-China_2_11.jpg" class="img_left" width="20%"> |
| <p style="margin-top:10%;margin-bottom:10%">First, draw a sketch on a small piece of paper with pencils and stick it on the back of a dish; </p> | | <p style="margin-top:10%;margin-bottom:10%">First, draw a sketch on a small piece of paper with pencils and stick it on the back of a dish; </p> |
− | <img src="human_practice_image/17.jpg" class="img_left" width="20%"> | + | <img src="https://static.igem.org/mediawiki/2015/8/87/WHU-China_2_17.jpg" class="img_left" width="20%"> |
| <p style="margin-top:20%;margin-bottom:10%">Second, lit an alcohol burner and clean their hands with 75% alcohol we provided; </p> | | <p style="margin-top:20%;margin-bottom:10%">Second, lit an alcohol burner and clean their hands with 75% alcohol we provided; </p> |
− | <img src="human_practice_image/18.jpg" class="img_left" width="20%"> | + | <img src="https://static.igem.org/mediawiki/2015/5/5b/WHU-China_2_18.jpg" class="img_left" width="20%"> |
| <p style="margin-top:20%">Third, pick out a toothpick near the alcohol burner, wet the toothpick with bacterium liquid and carefully copy your sketch to the dish ( patterns could be seen through the transparent dish ); </p> | | <p style="margin-top:20%">Third, pick out a toothpick near the alcohol burner, wet the toothpick with bacterium liquid and carefully copy your sketch to the dish ( patterns could be seen through the transparent dish ); </p> |
− | <img src="human_practice_image/19.jpg" class="img_left" width="20%"> | + | <img src="https://static.igem.org/mediawiki/2015/b/bf/WHU-China_2_19.jpg" class="img_left" width="20%"> |
| <p style="margin-top:10%;margin-bottom:13%">Forth, pick out another toothpick, select the other color if you like and repeat the last step.</p> | | <p style="margin-top:10%;margin-bottom:13%">Forth, pick out another toothpick, select the other color if you like and repeat the last step.</p> |
| </div> | | </div> |
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| <div class="member"> | | <div class="member"> |
| <div class="txt"> | | <div class="txt"> |
− | <img src="human_practice_image/21.jpg" width="20%" style="margin-top:2%" /> | + | <img src="https://static.igem.org/mediawiki/2015/8/8f/WHU-China_2_21.jpg" width="20%" style="margin-top:2%" /> |
| <p style="margin-top:10%;margin-bottom:10%">3.We talked with them on topics like microorganisms in our daily life and the development of synthetic biology. Moreover, we encouraged them to know more about iGEM online. </p> | | <p style="margin-top:10%;margin-bottom:10%">3.We talked with them on topics like microorganisms in our daily life and the development of synthetic biology. Moreover, we encouraged them to know more about iGEM online. </p> |
| </div> | | </div> |
| <div class="txt"> | | <div class="txt"> |
− | <img src="human_practice_image/20.jpg" width="20%" style="margin-top:4%" /> | + | <img src="https://static.igem.org/mediawiki/2015/7/73/WHU-China_2_20.jpg" width="20%" style="margin-top:4%" /> |
| <p style="margin-top:10%;margin-bottom:10%">4. All participants were informed to check their email several days later for photos of their sketch. Some participants even paid a visit to have a look at their dishes themselves. In this way, some of them were even shown how our lab works run.</p> | | <p style="margin-top:10%;margin-bottom:10%">4. All participants were informed to check their email several days later for photos of their sketch. Some participants even paid a visit to have a look at their dishes themselves. In this way, some of them were even shown how our lab works run.</p> |
| </div> | | </div> |
| <b>See in Next Year:</b> | | <b>See in Next Year:</b> |
| <div class="txt"> | | <div class="txt"> |
− | <img src="human_practice_image/22.jpg" width="20%" style="margin-top:1%;" /> | + | <img src="https://static.igem.org/mediawiki/2015/1/1b/WHU-China_2_22.jpg" width="20%" style="margin-top:1%;" /> |
− | <img src="human_practice_image/23.jpg" width="20%" style="margin-top:16%" /> | + | <img src="https://static.igem.org/mediawiki/2015/6/68/WHU-China_2_23.jpg" width="20%" style="margin-top:16%" /> |
| <p style="margin-top:10%;margin-bottom:11%">A lecture on some knowledge behind Sketch in Dish was held in the Community Activity Center several days later. At the beginning, we played some video clips to show them the beauty of life and attract them. Then, some principles of our experiments and sketch in dish were explained to those kids for a deeper understanding. To estimate how much they learnt, we asked some questions based on the lecture above. Lastly, we warmly welcomed them to participate in our activities ( human practices ) next year.</p> | | <p style="margin-top:10%;margin-bottom:11%">A lecture on some knowledge behind Sketch in Dish was held in the Community Activity Center several days later. At the beginning, we played some video clips to show them the beauty of life and attract them. Then, some principles of our experiments and sketch in dish were explained to those kids for a deeper understanding. To estimate how much they learnt, we asked some questions based on the lecture above. Lastly, we warmly welcomed them to participate in our activities ( human practices ) next year.</p> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="txt"> | | <div class="txt"> |
− | <img src="human_practice_image/8.jpg" width="30%" style="margin-top:1%" /> | + | <img src="https://static.igem.org/mediawiki/2015/5/53/WHU-China_2_8.jpg" width="30%" style="margin-top:1%" /> |
| <p style="margin-top:5%;margin-bottom:11%">In practice, we invited two graduate students in College of Stomatology who have the Practicing Doctors Certificate to do examination of oral cavity of participants in our university.</p> | | <p style="margin-top:5%;margin-bottom:11%">In practice, we invited two graduate students in College of Stomatology who have the Practicing Doctors Certificate to do examination of oral cavity of participants in our university.</p> |
| </div> | | </div> |
| <div class="txt"> | | <div class="txt"> |
− | <img src="human_practice_image/9.jpg" width="30%" style="margin-top:1%"/> | + | <img src="https://static.igem.org/mediawiki/2015/6/67/WHU-China_2_9.jpg" width="30%" style="margin-top:1%"/> |
| <p style="margin-top:15%;margin-bottom:15%">Students doing the examination.</p> | | <p style="margin-top:15%;margin-bottom:15%">Students doing the examination.</p> |
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| <div class="member"> | | <div class="member"> |
| <div class="txt"> | | <div class="txt"> |
− | <img src="human_practice_image/24.jpg" width="25%" style="margin-top:1%" /> | + | <img src="https://static.igem.org/mediawiki/2015/7/76/WHU-China_2_24.jpg" width="25%" style="margin-top:1%" /> |
| <p style="margin-top:15%;margin-bottom:20%"> | | <p style="margin-top:15%;margin-bottom:20%"> |
| All of the organizer and dentists | | All of the organizer and dentists |
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| <table width="600px" height="300px" style="margin:0"> | | <table width="600px" height="300px" style="margin:0"> |
| <tr> | | <tr> |
− | <th background="human_practice_image/25.jpg"></th> | + | <th background="https://static.igem.org/mediawiki/2015/e/e1/WHU-China_2_25.jpg"></th> |
− | <th background="human_practice_image/26.jpg"></th> | + | <th background="https://static.igem.org/mediawiki/2015/e/ed/WHU-China_2_26.jpg"></th> |
− | <th background="human_practice_image/27.jpg"></th> | + | <th background="https://static.igem.org/mediawiki/2015/4/41/WHU-China_2_27.jpg"></th> |
− | <th background="human_practice_image/28.jpg"></th> | + | <th background="https://static.igem.org/mediawiki/2015/f/f9/WHU-China_2_28.jpg"></th> |
| </tr> | | </tr> |
| <tr> | | <tr> |
− | <th background="human_practice_image/29.jpg"></th> | + | <th background="https://static.igem.org/mediawiki/2015/9/99/WHU-China_2_29.jpg"></th> |
− | <th background="human_practice_image/30.jpg"></th> | + | <th background="https://static.igem.org/mediawiki/2015/f/fb/WHU-China_2_30.jpg"></th> |
− | <th background="human_practice_image/31.jpg"></th> | + | <th background="https://static.igem.org/mediawiki/2015/1/1c/WHU-China_2_31.jpg"></th> |
− | <th background="human_practice_image/32.jpg"></th> | + | <th background="https://static.igem.org/mediawiki/2015/8/83/WHU-China_2_32.jpg"></th> |
| </th> | | </th> |
| </table> | | </table> |