Difference between revisions of "Team:Birkbeck/Results"

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<!--<p>In order to investigate if there could be a point in the <i>E. coli</i> DH5α growth curve in which a signal from the GFP could be detected by absorbance, the growth curves were also conducted using the major absorption peak of GFP (wavelength 395 nm) (REF!). The growth curve data for the culture optical density is displayed in Fig. 2.</P>-->
  
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<p><b><u>Fig. 3: Viable Count of <i>E. coli</i> DH5α After 60 mins</u></b>.</p>
 
<p><b><u>Fig. 3: Viable Count of <i>E. coli</i> DH5α After 60 mins</u></b>.</p>
 
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<td class=" e dataAreaTop dataAreaLeft vCC">9</td>
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<td class=" e dataAreaTop vCC">1600000</td>
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<td class=" e dataAreaTop vCC">3750000</td>
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<td class=" e dataAreaTop vCC">2566666.67</td>
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<td class="rowLabels hCR role3">Valid N (listwise)</td>
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<td class=" o dataAreaLeft hCR vCC">9</td>
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<p><b><u>Table 1: Descriptive Statistics of 60 minutes Viable Count</u></b>.</p>
 
<center><img src="https://static.igem.org/mediawiki/2015/e/e6/Viable_count_of_DH5_alpha_after_175_mins_team_birkbeck_iGEM_2015.jpg"></center>
 
<center><img src="https://static.igem.org/mediawiki/2015/e/e6/Viable_count_of_DH5_alpha_after_175_mins_team_birkbeck_iGEM_2015.jpg"></center>
 
<p><b><u>Fig. 4: Viable Count of <i>E. coli</i> DH5α After 175 mins</u></b>.</p>
 
<p><b><u>Fig. 4: Viable Count of <i>E. coli</i> DH5α After 175 mins</u></b>.</p>
 
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<center><IMG SRC="https://static.igem.org/mediawiki/2015/8/8a/Growth_curve_601_nm_96-microtitre_Team_birkbeck_iGEM_2015_data.jpg "></center>
 
<center><IMG SRC="https://static.igem.org/mediawiki/2015/8/8a/Growth_curve_601_nm_96-microtitre_Team_birkbeck_iGEM_2015_data.jpg "></center>
 
<p><b><u>Fig. 5: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Optical Density at 601 nm</u></b>.</p>
 
<p><b><u>Fig. 5: Growth Curves of Different Strains of <i>E. coli</i> DH5α Following Culture Optical Density at 601 nm</u></b>.</p>

Revision as of 20:02, 6 September 2015

iGEM Westminster Logo




Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!




Under Construction

Fig. 1: Growth Curve of E. coli DH5α Strains Following Culture Optical Density of 600 nm.


Fig. 2: Growth Curve of E. coli DH5α Strains Following Culture Optical Density of 395 nm.



Fig. 3: Viable Count of E. coli DH5α After 60 mins.


Descriptive Statistics of 1 hour Viable Count.
  N Minimum Maximum Mean Std. Deviation
Viable.Count 9 1600000 3750000 2566666.67 627495.020
Valid N (listwise) 9        

Table 1: Descriptive Statistics of 60 minutes Viable Count.

Fig. 4: Viable Count of E. coli DH5α After 175 mins.


Fig. 5: Growth Curves of Different Strains of E. coli DH5α Following Culture Optical Density at 601 nm.


Fig. 6: Growth Curves of Different Strains of E. coli DH5α Following Culture Optical Density at 501 nm.


Fig. 7: Growth Curves of Different Strains of E. coli DH5α Following Culture Optical Density at 475 nm.


Fig. 8: Growth Curves of Different Strains of E. coli DH5α Following Culture Optical Density at 395 nm.


Fig. 9: Growth Curves of Different Strains of E. coli DH5α Following Culture Fluorescence.