Difference between revisions of "Team:NTU-LIHPAO-Taiwan/Basic Part"

Revision as of 07:27, 7 September 2015 (view source) Aladeen (Talk | contribs) ← Older edit		Revision as of 07:28, 7 September 2015 (view source) Aladeen (Talk | contribs) Newer edit →
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	<div class="Text3">		<div class="Text3">
	<ol class="part2">		<ol class="part2">
−	<li>
−	Table
	<table>		<table>
	<tr>		<tr>
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	</tr>		</tr>
	</table>		</table>
−	&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Molar ratio: insert/vector = 3/1
−	<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; x + y = 6</br>
−	</li>
−	<li>Gently mix the solution by pipetting up and down</li>
−	<li>Incubate
−	<ol class="part3">
−	<li>at 16℃ for 2 hours, or</li>
−	<li>at 37℃ for 1 hour, or</li>
−	<li>at 4℃ overnight</li>
−	</ol>
−	</li>
−	<li>Proceed with bacterial transformation</li>
	</ol>		</ol>
	</div>		</div>

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Revision as of 07:28, 7 September 2015

• Home
• Team
• Project
  • Abstract
  • Overview
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  • Results
• Modeling
  • Abstract
  • Simulation
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• Notebook
  • Note
  • Protocols
• Safety
• Human Practice
  • Sub Item
  • Sub Item
  • Sub Item

• Notes
  • Preparation of Competent Cells (E.coli)
  • DNA Dissolution
  • Agarose Gel Electrophoresis
  • Gel Extraction
  • DNA Ligation
  • Plasmid DNA Extraction Using Alkaline Lysis Method (E.coli)
  • Transformation (E.coli)

DNA Ligation

Promoter :

Components	Volume (μL)
Insert	x
Vector	y
Ligation High	1
Total	7

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