Difference between revisions of "Team:NTU-LIHPAO-Taiwan/Basic Part"

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	</tr>		</tr>
	</table>		</table>
		+	</li>
		+	<li>Gently mix the solution by pipetting up and down</li>
		+	<li>Incubate
		+	<ol class="part3">
		+	<li>at 16℃ for 2 hours, or</li>
		+	<li>at 37℃ for 1 hour, or</li>
		+	<li>at 4℃ overnight</li>
		+	</ol>
		+	</li>
		+	<li>Proceed with bacterial transformation</li>
	</ol>		</ol>
	</div>		</div>

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Revision as of 07:28, 7 September 2015

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• Notes
  • Preparation of Competent Cells (E.coli)
  • DNA Dissolution
  • Agarose Gel Electrophoresis
  • Gel Extraction
  • DNA Ligation
  • Plasmid DNA Extraction Using Alkaline Lysis Method (E.coli)
  • Transformation (E.coli)

DNA Ligation

Promoter :

Components	Volume (μL)
Insert	x
Vector	y
Ligation High	1
Total	7

1. Gently mix the solution by pipetting up and down
2. Incubate
   1. at 16℃ for 2 hours, or
   2. at 37℃ for 1 hour, or
   3. at 4℃ overnight
3. Proceed with bacterial transformation

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