Difference between revisions of "Team:Czech Republic/Notebook"
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* [[--|PCR]] - annealing temperature was 55°C, 30 cycles | * [[--|PCR]] - annealing temperature was 55°C, 30 cycles | ||
| − | Gel electrophoresis - verification of successfull amplification of ste2 | + | * [[--|Gel electrophoresis]] - verification of successfull amplification of ste2 |
| + | |||
| + | * [--|Restriction digest]] - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII | ||
== Week 2 (11-17) == | == Week 2 (11-17) == | ||
Revision as of 08:56, 7 September 2015
Contents
Notebook
May
Week 1 (4-10)
Module 2
Monday
PCR of the ste2 gene from the genome of Saccharomyces cerevisiae 7284 strain.
- Genome extraction
- PCR - annealing temperature was 55°C, 30 cycles
- Gel electrophoresis - verification of successfull amplification of ste2
- [--|Restriction digest]] - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII
Week 2 (11-17)
Week 3 (18-24)
Week 4 (25-31)
June
July
August
September