Difference between revisions of "Team:Czech Republic/Notebook"
(→Monday) |
(→Module 2) |
||
| Line 16: | Line 16: | ||
* [[--|Restriction digest]] - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII | * [[--|Restriction digest]] - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII | ||
| + | |||
| + | === Tuesday === | ||
| + | * [[--|Gel electrophoresis]] - pRSII416 | ||
| + | * [[--|Gel purification]] - | ||
| + | * [[--|Ligation]] - | ||
| + | * [[--|Transformation]] - | ||
== Week 2 (11-17) == | == Week 2 (11-17) == | ||
Revision as of 09:02, 7 September 2015
Contents
Notebook
May
Week 1 (4-10)
Module 2
Monday
PCR of the ste2 gene from the genome of Saccharomyces cerevisiae 7284 strain.
- Genome extraction
- PCR - annealing temperature was 55°C, 30 cycles
- Gel electrophoresis - verification of successfull amplification of ste2
- Restriction digest - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII
Tuesday
- Gel electrophoresis - pRSII416
- Gel purification -
- Ligation -
- Transformation -
Week 2 (11-17)
Week 3 (18-24)
Week 4 (25-31)
June
July
August
September