Difference between revisions of "Team:Czech Republic/Notebook"
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* [[--|Gel electrophoresis]] - verification of successfull amplification of ste2 | * [[--|Gel electrophoresis]] - verification of successfull amplification of ste2 | ||
[[File:Czech_Republic_M2_gel-05-04.png|250px]] | [[File:Czech_Republic_M2_gel-05-04.png|250px]] | ||
| + | 1.3kb | ||
* [[--|Restriction digest]] - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII | * [[--|Restriction digest]] - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII | ||
Revision as of 10:59, 7 September 2015
Contents
Notebook
May
Week 1 (4-10)
Module 2
Monday
PCR of the ste2 gene from the genome of Saccharomyces cerevisiae 7284 strain.
- Genome extraction
- PCR - annealing temperature was 55°C, 30 cycles
- Gel electrophoresis - verification of successfull amplification of ste2
1.3kb
- Restriction digest - restriction of the amplified ste2 gene and pRSII416 vector with BamHI + HindIII
Tuesday
- Gel electrophoresis - pRSII416 from this Monday
- Gel purification - extraction and purification of the digested pRSII416 vector
- Ligation -
- Transformation -
Week 2 (11-17)
Week 3 (18-24)
Week 4 (25-31)
June
July
August
September