Difference between revisions of "Team:Leicester/Measurement"

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           <p>As a team we decided that we would do some extra work during our time doing the iGEM project this such that we entered to take part in the Interlab Measurement study. The study requires us to look at the fluorescence of given gene circuits made up of parts found in the 2015 Distribution Kits.</p>
 
           <p>As a team we decided that we would do some extra work during our time doing the iGEM project this such that we entered to take part in the Interlab Measurement study. The study requires us to look at the fluorescence of given gene circuits made up of parts found in the 2015 Distribution Kits.</p>
  
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<p> The study requires producing 3 devices each consisting of a promoter sequence (depicted as different coloured arrows on Figure 1) and Green Fluorescence Protein (GFP) intermediate. Each of the devices are shown below: </p>
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<p> The study requires producing 3 devices each consisting of a promoter sequence (depicted as different coloured arrows on Figure 1) and Green Fluorescence Protein (GFP) intermediate (I13504). Each of the devices are shown below: </p>
 
<img src="https://static.igem.org/mediawiki/2015/d/d7/Leicester_Device_gene_circuits.jpg" align="right">
 
<img src="https://static.igem.org/mediawiki/2015/d/d7/Leicester_Device_gene_circuits.jpg" align="right">
 
<ul>
 
<ul>

Revision as of 16:19, 7 September 2015

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Overview

As a team we decided that we would do some extra work during our time doing the iGEM project this such that we entered to take part in the Interlab Measurement study. The study requires us to look at the fluorescence of given gene circuits made up of parts found in the 2015 Distribution Kits.

The study requires producing 3 devices each consisting of a promoter sequence (depicted as different coloured arrows on Figure 1) and Green Fluorescence Protein (GFP) intermediate (I13504). Each of the devices are shown below:

  • Device 1 - J23101 + I13504

  • Device 2 - J23106 + I13504

  • Device 3 - J23117 + I13504



As part of the Interlab study, we then did extra credit where we had get biological and technical replicates of each device. They have been defined as such:

  • Biological Replicate -

  • Technical Replicate -

Methodology

In order to get the

Results

Using the Omega Fluostar Fluormeter, we were able to look at the fluorescence of the different devices. It was found that the

Discussion and Difficulties