Difference between revisions of "Team:Yale"

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        <p>Not your typical role model. <a href="#">Learn more.</a></p>
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      <div class="small-7 columns"><img src="http://client.cameronyick.us/igem/assets/img/iGEM_Tree_Round.png"></div>
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        <p class="hero__desc">Developing a Framework for the Genetic Manipulation of Non-Model and Environmentally Significant Microbes</p>
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        <h1 class="hero__head">Yale iGem</h1>
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        <div class="text-center">
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          <h2 class="hero__year">2015</h2><a class="custom__button pdf__button">PDF Summary</a>
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            <h2 class="section__head">Project Abstract</h2>
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            <p class="abstract__text">We focused on establishing a framework to implement genetic manipulation techniques—specifically, multiplex automated genome engineering (MAGE) and CRISPR-Cas9 systems—into non-model, environmentally significant microbes using standard biological parts. The framework involves two components: (1) propagation and selection of cultures and (2) manipulation of cell genomes by MAGE and/or CRISPR. We identified design considerations for both components of the framework, and experimentally validated propagation and selection considerations using cyanobacterial strain Synechococcus sp. PCC 7002 (a fast-growing cyanobacterium capable of lipid biofuel production) and Rhizobium tropici CIAT (a nitrogen-fixing rhizobium which forms root nodules in legume plants). We then developed a workflow for the design, construction, and testing of MAGE and CRISPR technologies into non-model prokaryotes. The insights we gained from validating the propagation component of our workflow will serve to improve the versatility and robustness of our framework and will inform the development of tools for genetic manipulation in other non-model organisms.</p>
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      <h2 class="light__head">Find Out More</h2>
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          <h2><a href="project" alt="Project">Project</a></h2>
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          <h2><a href="notebook" alt="Notebook">Notebook</a></h2>
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          <h2><a href="collaborations" alt="Collaborations">Collaborations</a></h2>
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          <h2><a href="team" alt="Team">Team</a></h2>
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          <h2><a href="practices" alt="Human Practices">Human Practices</a></h2>
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          <h2><a href="standards" alt="iGem Standards">iGem Standards</a></h2>
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            <h2 class="section__head">Contact Us: </h2>
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            <h3>Undergraduate Researchers</h3>
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            <p>Colin Hemez: colin.hemez@yale.edu</p>
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            <p>Lionel Jin: chentian.jin@yale.edu</p>
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            <p>Danny Keller: daniel.keller@yale.edu</p>
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            <p>Dan Shapiro: daniel.m.shapiro@yale.edu</p>
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            <p>Jessica Tantivit: jessica.tantivit@yale.edu</p>
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            <p>Erin Wang: erin.wang@yale.edu</p>
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            <p>Holly Zhou: holly.zhou@yale.edu</p>
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            <h3>Graduate Student Mentor</h3>
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            <p>Natalie Ma (lead mentor): natalie.ma@yale.edu</p>
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            <h2 class="section__head">igem.yale@gmail.com</h2>
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            <h3>iGEM Team Board</h3>
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            <p>Ed Kong (president): edward.kong@yale.edu</p>
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            <p>Alex Buhimschi (vice-president): alexandru.buhimschi@yale.edu</p>
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            <p>Stephanie Mao (outreach chair): stephanie.mao@yale.edu</p>
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            <h3>Faculty Advisors</h3>
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            <p>Farren Isaacs (Yale MCDB): farren.isaacs@yale.edu</p>
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            <p>Stephen Dellaporta (Yale MCDB): stephen.dellaporta@yale.edu</p>
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            <h2>Yale iGem 2015</h2>
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            <p>Main Campus:</p>
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            <p>Yale Department of Molecular, Cellular & Developmental Biology</p>
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            <p>Attn: Farren Isaacs/iGEM</p>
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            <p>219 Prospect St</p>
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            <p>PO Box 208103</p>
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            <p>New Haven, CT 06520</p>
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            <p>Tel. +1(203) 432-3783</p>
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            <p>E-mail: igem.yale@gmail.com</p>
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          <p class="text-center">&copy; Yale iGEM 2015</p>
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<h1>Overview<h1>
 
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<p>Our iGEM research project involves porting Multiplex Automated Genome Engineering (MAGE) technology into two prokaryotic organisms—Sinorhizobium meliloti 1021 and Synechococcus sp. PCC 7002—for the production of industrially-relevant small molecules. MAGE was developed as a rapid, high efficiency tool for increasing the genetic diversity of a cell population at targeted loci within the genome. The technique has so far been ported into the model organism Escherichia coli and a few other members of the family Enterbacteriaceae. Sinorhizobium meliloti 1021 is a nitrogen-fixing bacterium capable of forming root nodules with legume plants. Synechococcus sp. PCC 7002 is a fast-growing marine cyanobacterium capable of photosynthesis. We envision numerous potential applications for MAGE in these organisms; for example, the nitrogen fixation mechanisms in Sinorhizobium meliloti 1021 could be modified to enable plant growth in otherwise hostile environments, and the lipid biosynthesis pathway of Synechococcus sp. PCC 7002 could be optimized for the production of molecules that serve as precursors to lipid biofuels.<p>
 
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Revision as of 01:00, 8 September 2015


Yale iGem 2015: NonModel

Not your typical role model. Learn more.

Developing a Framework for the Genetic Manipulation of Non-Model and Environmentally Significant Microbes

Yale iGem

2015

PDF Summary

Project Abstract

We focused on establishing a framework to implement genetic manipulation techniques—specifically, multiplex automated genome engineering (MAGE) and CRISPR-Cas9 systems—into non-model, environmentally significant microbes using standard biological parts. The framework involves two components: (1) propagation and selection of cultures and (2) manipulation of cell genomes by MAGE and/or CRISPR. We identified design considerations for both components of the framework, and experimentally validated propagation and selection considerations using cyanobacterial strain Synechococcus sp. PCC 7002 (a fast-growing cyanobacterium capable of lipid biofuel production) and Rhizobium tropici CIAT (a nitrogen-fixing rhizobium which forms root nodules in legume plants). We then developed a workflow for the design, construction, and testing of MAGE and CRISPR technologies into non-model prokaryotes. The insights we gained from validating the propagation component of our workflow will serve to improve the versatility and robustness of our framework and will inform the development of tools for genetic manipulation in other non-model organisms.

Find Out More

Project

Notebook

Collaborations

Team

Human Practices

iGem Standards

Contact Us:

Undergraduate Researchers

Colin Hemez: colin.hemez@yale.edu

Lionel Jin: chentian.jin@yale.edu

Danny Keller: daniel.keller@yale.edu

Dan Shapiro: daniel.m.shapiro@yale.edu

Jessica Tantivit: jessica.tantivit@yale.edu

Erin Wang: erin.wang@yale.edu

Holly Zhou: holly.zhou@yale.edu

Graduate Student Mentor

Natalie Ma (lead mentor): natalie.ma@yale.edu

igem.yale@gmail.com

iGEM Team Board

Ed Kong (president): edward.kong@yale.edu

Alex Buhimschi (vice-president): alexandru.buhimschi@yale.edu

Stephanie Mao (outreach chair): stephanie.mao@yale.edu

Faculty Advisors

Farren Isaacs (Yale MCDB): farren.isaacs@yale.edu

Stephen Dellaporta (Yale MCDB): stephen.dellaporta@yale.edu

Yale iGem 2015

Main Campus:

Yale Department of Molecular, Cellular & Developmental Biology

Attn: Farren Isaacs/iGEM

219 Prospect St

PO Box 208103

New Haven, CT 06520

Tel. +1(203) 432-3783

E-mail: igem.yale@gmail.com

© Yale iGEM 2015


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