Difference between revisions of "Team:ZJU-China/Notebook"

Revision as of 06:37, 8 September 2015

Timeline

August
9.5.2015

Finished the overview of entire project.
9.4.2015
- Bacteria solution PCR: OrfX. One positive clone of 20 samples.
- DNA sequencing: OrfX.
9.3.2015

Watched the military parade during the commemoration activities to mark the 70th anniversary of the victory of the Chinese People's War of Resistance Against Japanese Aggression and the World Anti-Fascist War in our lab.
9.2.2015

Dynamic Light Scattering(DLS) experiment to measure the particle size of embedded E.coli.
9.1.2015

The sequence of OrfX was not correct because of the wrong template, thus we had to do the related experiments again.
8.31.2015

Took photos and prepared for the banner~
8.30.2015

The sequence of metK and tcdA1 were confirmed, which marked the success in standardization of 2 parts!!
8.29.2015

Bacteria solution PCR: metK and OrfX. Both had positive clones. DNA sequencing: metK and OrfX. T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3. Selected from single colonies: CDS-tcdA1.
8.28.2015
- Selected from single colonies of metK and OrfX.
- Submit the safety form. Selected from single colonies: CDS-plu0840. Double enzyme digestion and DNA gel extraction: plu1537, tcdA1, plu0840. Plasmid extration and double enzyme digestion: RFP. Protein extraction and SDS-PAGE: tcdA1, plu1537, plu0840. T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3. Bacteria solution PCR: CDS-plu0840.
8.27.2015

Seamless assembly: plasmid+OrfX, plasmid+metK. PCR: CDS-tcdA1. Double enzyme digestion: RFP+plasmid. Seamless assembly: CDS-plu0840. PCR and DNA gel extraction: CDS-tcdA1. Changed pSB1A3 to pSB1C3: plu0840, plu1537, tcdA1. Transformed them into E.coli DH5α, selected from single colonies and used single enzyme digestion to verify the got plasmids.
8.26.2015

Seamless assembly: plasmid+CDS-tcdA1, plasmid+CDS-plu0840. DNA gel extraction: tcdA1. PCR: device-plu0840.
8.25.2015

The sequence of ermE+plasmid, Frr+plasmid and plu1537+plasmid were confirmed, which marked the success in standardization of 3 parts!!
8.24.2015
- Bacteria solution PCR: metK and OrfX. Only negative clone. Extraction of plasmid mCherry and double enzyme digestion. DNA sequencing: ermE+plasmid, Frr+plasmid, plu1537+plasmid. Selected from single colonies of plu1537.
- Power cut. We played table tennis near our lab.
8.23.2015
- Selected from single colonies of metK and OrfX.
- Transformed standard plu1537 plasmid into BL-21. Bacterium solution PCR: ermE and Frr.
8.22.2015
- Selected from single colonies of ermE and Frr. Extraction of plasmid (standard plu1537).
- Bacterium solution PCR: standard plu1537.
8.21.2015
- DNA sequencing. Seamless assembly: plasmid+1537, plasmid+tcdA1-L+ tcdA1-M+ tcdA1-R, plasmid+ermE, plasmid+Frr.
- Successfully booked the HI-Boston Hostel and paid the full payment~ Seamless assembly: plasmid+metK, plasmid+OrfX.
38th Group meeting

8.20.2015

38th Group meeting. Introduced a software named Mou which could enhance the efficiency of writing wiki. Discussed the business planning of our product and the photos on the wiki.
8.20.2015
- Extraction of plasmid mCherry and double enzyme digestion. Seamless assembly: plasmid+1537, plasmid+tcdB1. PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction.
- Capsulated Streptomyces Avermitilis toxicity test. TT01 toxicity verification.
- PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction.
- Again used flow cytometry to check the diameter of the embedded E.coli and tried to count the E.coli which were successfully embedded. The results were not that ideal again.
8.19.2015
- PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction.
- Finished the background of avermectin and toxic protein. Sent bacterium sample to BNU and we would continue to help them.
8.18.2015
- PCR: CDS tcdB1, CDS 1537, tcdA1-L, mcherry. DNA sequencing.
- Half-done the model of degration of toxic protein. Got the kit for detection avermectin.
8.17.2015
- PCR: CDS tcdB1, CDS plu1537 and mCherry. DNA gel extraction: tcdA1-L and plu0840L. Seamless assembly: plasmid+pBad+plu1537.
- Detected the relationship between distance of food source and food consumption.
- Designed the primers for Frr, metK, OrfX and ermE again because the need for standardization.
37th Group meeting

8.16.2015

37th Group meeting. Built the framework for wiki. Discussed the Syn-bio talks on next Saturday.
8.16.2015
- Preparation of competent cells.DNA gel extraction.
- Verification of the bait attractiveness.
8.15.2015
- PCR: pBad, tcdA1-L, tcdB1, plu0840L and plu1537. Purchased E.coli BL21.
- The experiment concerning Streptomyces avermitilis finished 80%.
8.14.2015
- PCR and clean up.
- Test the Avermectin detection kit with pure Ivermectin.
- Designed the primers for plu0840, plu1537, tcdA1 and tcdB1 again bucause of the need for standardization.
8.13.2015
- Overlap: pBad and tcdA1-L, pBad and tcd-B1, pBad and plu1537. Extraction of plasmid mCherry and enzyme digestion.
- Completed wiki page for 2nd model. Drew the 3D ball-and-stick models for CNC with Solidworks. Completed the device for killing termites.
- Completed the SCM program for HUST and they affirmed our help.
8.12.2015
- Overlap: 0840L and 0840R.
- Tried to purify the embedded E.coli with the E.coli which were able to express RFP.
- Used flow cytometry to check the diameter of the embedded E.coli and tried to count the E.coli which were successfully embedded. The results were not that ideal.
8.11.2015
- PCR: pBad, tcdA1-L, tcdA1-M, tcdA1-R, tcdB1, 0840L, 0840R, 1537, GFP.
- PCR: ligated product and optimized the PCR program. Cleaned up. PCR again.
- SEM scanned our samples to verify whether Streptomyces avermitilis were successfully embedded with CNC.
- Fed the termites with Photorhabdus luminescens TT01 bacterium cells.
8.10.2015
- PCR: pBad, tcdA1-L and GFP.
- Used Bgl2 and EcoR1 digested inserted fragment and cleaned up the product. Ligating fragment into plasmid with T4 ligase.
- Specified the standardization requirements and laid the foundation for primer designing.
- Mixed blue-stained termites with red-stained termites to verify trophallaxis.
35th Group meeting

8.9.2015

35th Group meeting. Planned to hold a seminar for freshmen. Tried to design a device for attracting and killing termites.
8.9.2015
- PCR: tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R and plu1537.
- Completed our framework and philosophy of safety.
- Extracted the plasmid containing RFP from DH5α. PCR: RFP and confirmed by gel electrophoresis.
8.8.2015

Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).
8.7.2015

One step cloning protocol finished. Designed the questionaire about termites.
8.7.2015-8.8.2015

PCR: pBad, GFP, tcdA1-M, tcdA1-R.
34th Group meeting

8.6.2015

34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety.
8.6.2015
- Drawing plasmid circuits in snapgene.(toxic protein)
- Stained 50 termites in blue and 50 in red for trophallaxis experiments.
8.5.2015
- Discussed the circuit construction and experiments planning of toxic protein.
- Decided to give up confocol because we couldn't find the specific dye for CNC.
8.4.2015

Completed the One Step Cloning protocol.
33rd Group meeting

8.2.2015

33rd Group meeting. Began to build framework for wiki. Introduced the habits and characteristics of termites.
July
32nd Group meeting

7.30.2015

32nd Group meeting. Planned to promote synthetic biology and our project in Shanghai Science & Technology Museum. Polished up the Synbio-cards(Polypoly card).
7.28.2015-8.4.2015

PCR: pBad, tcdA1-L, tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R, plu1537 and GFP.
7.28.2015

Giant Jamboree registration completed.
7.26.2015
- Listed 15 main problems we were confronted with, including safety, communication between dry group and wet groups.
- Designed a new method to embed the bacterium with CNC.
7.25.2015

Designed the primers for plu0840, plu1537, tcdA1 and tcdB1 again bucause of the bug of extra RBS.
7.24.2015

Discussed helping BNU and HUST. Designed and alpha tested the Synbio-cards. Improved the modeling.
7.19.2015-7.24.2015

Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST.
7.18.2015
- Listed and detailed the wiki requirements.
- Fed the termites with Streptomyces Avermitilis bacterium cells.
7.16.2015

Prepared the home page and member page of our wiki. Prepared the poster for NCTU meetup.
7.15.2015
- Designed the primers for Frr, metK, OrfX and ermE again. Got our name card~
- Used confocal to verify the successful package of E.coli with CNC. However, the result appeared not ideal and we couldn't find appropriate dye of CNC.
7.14.2015

Prepared for the souvenirs for NCTU meetup.
31st Group meeting

7.12.2015

31st Group meeting. Reviewed the medal requirement and all the rules in lab.
7.11.2015

Mark-recapture method failed because we failed to recapture enough stained termites.
7.3.2015-7.11.2015

Exams of summer semester.
June
30th Group meeting

6.25.2015

30th Group meeting. Planned to verify the package of CNC with confocal. Continued to prepare for the presentation and application scenario.
6.24.2015
- Completed 2 safety questionaires.
- Prepared to learn confocol to verify the CNC package for E.coli.
6.22.2015

Put 150 blue stained termites back into the nest for mark-recapture method.
29th Group meeting

6.21.2015

29th Group meeting. Discussed the application scenario for the 5000€ additional sponsership of SYNENERGENE.
6.21.2015

Contacted with Björn and he would help us animate the process of crystallization.
6.20.2015

TEM scanned our samples.
6.19.2015

Fed the termites with Photorhabdus luminescens TT01 bacterium solution to test the natural toxicity. Stained 150 termites in blue for future experiment.
28th Group meeting

6.18.2015

28th Group meeting. 2nd modeling half done. Discussed the results of the SEM.
6.17.2015

TEM and SEM scanned our samples.
6.15.2015

Fed the termites with Streptomyces Avermitilis bacterium solution to test the natural toxicity.
27th Group meeting

6.14.2015

27th Group meeting. Rearranged all the events with four quadrant method. Checked everything we have had.
6.14.2015

Solved the diffusion equation.
6.13.2015

Extracted the genome of Streptomyces avermitilis.
6.12.2015

Went to shanghai for the interview of visa. 12 members successfully passed the interview.
26th Group meeting

6.11.2015

26th Group meeting. Discussed the half-done presentation and Skype conference with SYNENERGENE. Prepared for the visa.
6.11.2015

PCR: plu1537, with the template of the genome of TT01.
6.10.2015
- Paid for the air tickets.
- Got the primers of toxic protein.
6.8.2015

Optimization of the model of termites finding food.
25th Group meeting

6.7.2015

25th Group meeting. Polished up the previous modeling. Protocols about the experiments concerning bacterium. A member.
6.7.2015

Extracted the genome of TT01.
6.6.2015

Frozen the spores of Streptomyces avermitilis.
24th Group meeting

6.4.2015

24th Group meeting. Brainstormed the Synbio-cards and Synbio-workshops. Listed the datailed personal scheme about the time arrangement before summer holiday.
6.3.2015
- Discussed the characterization of E.coli embedded in CNC, including TEM, SEM, confocal, FTIR and flow cytometry.
- Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE! Cheers~
- Brainstormed how to do human practice and use the €5000. We came up with the Synbio-cards and Synbio-workshops.
6.2.2015

Began to book the air tickets. Booked the first primers of toxic protein for one step cloning. Found the sequence of the gene we need in the Streptomyces avermitilis.
6.1.2015

SEM scanned our samples again. Debugged the first modeling.
May
23rd Group meeting

5.31.2015

23rd Group meeting. Discussed the half-done modeling.
5.31.2015

Taught high school students to do experiment to extract DNA from epithelial cell of mouth .Built up the model of termites finding food.
5.29.2015
- Repeated the cellulase activity detection experiment. Meanwhile we measured the lysozyme activity in different parts of termites guts.
- 22nd Group meetig. Specified the medal requirement. Began to prepare for the NCTU meetup.
- SEM scanned our samples.
5.28.2015

Completed a model of a cartoon termite holding a sword. Cleaned up our lab and rearranged all the experiment materials we would need.
5.26.2015

Got the solid CNC with lyophilization. Built the framework of the first web page.
5.25.2015

Learned the skills of culturing Streptomyces avermitilis in the professor's lab.
21st Group meeting

5.24.2015

21st Group meeting. Began to prepare for the presentation.
5.22.2015

iGEM Shanghai Tour. Took part in the conference held in NYU. Visited FDU and communicated with them.
5.21.2015

Chosen the certain toxic protein which we would transfer into E.coli: tcdA1, tcdB1,plu0840 and plu1537.
5.21.2015

20th Group meeting. Discussed the medal requirements and collaboration among groups.
5.20.2015
- Finished the protocol about TT01.
- Measured the cellulase activity in termites head, forgut, midgut and hindgut.
5.19.2015

Detailed the medal requirements depending on our project. Finished the protocol about Streptomyces avermitilis.
5.18.2015

Got the E.coli strain ET12567 from Professor Li's Lab. Began the experiment about Streptomyces avermitilis.
18th Group meeting

5.17.2015

18th Group meeting. Emphasized the lab safety.
5.17.2015

Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio.
5.15.2015

Read paper and discussed how to enhance the efficiency of Avermectin in Streptomyces avermitilis.
17th Group meeting

5.14.2015

17th Group meeting. Discussed the results of the TEM. Tried to contact with a judge from PKU.
5.14.2015
- TEM scanned our samples: using CNC to pack the Bacillus subtilis.
- Completed the cover and comic of our proposal which would be handed in to SYNENERGENE.
- Contacted with Hangzhou Termites control Institute.
5.13.2015
- Got the genome of tcdA1 and tcdB1 in NCBI.
- Got TT01(a species of Photorhabdus luminescens) which could produce toxic protein. It would play an important role in our plan B.
5.12.2015

Successfully checked in termites and tc protein family.
16th Group meeting

5.10.2015

16th Group meeting. Communication between dry group and 3 wet groups. Arranged time for experiments.
15th Group meeting

5.7.2015

15th Group meeting. Further discussed the proposal and allocated the tasks. Continued to consult professors about several species of bacterium.
5.6.2015
- Successfully got the solution of CNC!! And it appeared Tyndall effect.
- Gave up synthesizing avermectin in Bacillus subtilis. Tried to overexpress avermectin in E.coli and then transformed its plasmid into Streptomyces avermitilis.
5.4.2015-5.6.2015

Exams of spring semester.
5.3.2015

Our termite nest was sent out from Guangdong Province.
April
14th Group meeting

4.29.2015

14th Group meeting. 10 members would go to Taiwan for NCTU meetup. Results of consulting professors specialized in termites and Bacillus subtilis.
4.28.2015
- Discussed the plan for embedding baterium with CNC and the self-assembly of CMC and chitosan on the surface of baterium.
- Debugged the problem of synthesizing avermectin in Streptomyces avermitilis. Consulted professors specialized in synthesizing avermectin in Streptomyces avermitilis.
4.27.2015

Consulted professors specialized in termites and Bacillus subtilis.
13th Group meeting

4.26.2015

13th Group meeting. Discussed something important: the proposal for the 5000€ additional sponsership of SYNENERGENE and visa. A new mamber joined our team. Specified the problem remaining to be solved of each group.
4.25.2015

Discussd the advantage of Bacillus subtilis campared with E.coli and the utilization of spore of Bacillus subtilis.
12th Group meeting

4.23.2015

12th Group meeting. Debugged the previous package plan and decided to pack bacterium with CNC. Planned to buy a nest of termites from Guangdong Province. Discussed several demensions of modeling in this project.
4.22.2015

Consulted professors specialized in termites.
4.21.2015

Consulted professors about directly embedding baterium with CNC rather than with the aid of protein domain. Our idea was confirmed and praised.
4.20.2015

Communication with our instructor. Searched for information about cellulose binding domain(CBDs) and overexpression of ivermectin and avermectin.
11th Group meeting

4.19.2015

11th Group meeting. Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project.
10th Group meeting

4.16.2015

10th Group meeting. Decided to participate in NCTU meetup in summer holiday and apply for the 5000€ additional sponsership of SYNENERGENE. Discussed 3 existing ideas.
4.14.2015

Preliminarily designed the circuit in Streptomyces avermitilis.
9th Group meeting

4.12.2015

9th Group meeting. Kept 3 ideas and the final idea would derive from them. Detailed these 3 ideas from all the aspects.
8th Group meeting

4.9.2015

8th Group meeting. Continued to kill unappropriate ideas. Recalled the aim of participating iGEM and reached a consensus.
7th Group meeting

4.6.2015

7th Group meeting. Began to kill some undoable ideas and further dug the existing ideas, such as biological enigma machine and killing termites.
6th Group meeting

4.2.2015

6th Group meeting. Developed several new ideas, including bio-SCM, biological enigma machine and slow release of certain chemical. Talked about their feasibility.
March
5th Group meeting

3.29.2015

5th Group meeting. Dug previous ideas and considered their safety and feasibility.
4th Group meeting

3.26.2015

4th Group meeting. Continued to share and improve our ideas, such as bio-lens, detection of schistosomiasis and killing termites.
3.25.2015

Team application completed.
3rd Group meeting

3.22.2015

3rd Group meeting. Discussed some ideas about modeling. Shared new ideas.
3.21.2015

Communicate with Zhejiang Science&Technology Museum.
2nd Group meeting

3.19.2015

2nd Group meeting. Built up several rules and principles. Tried to make the most of our BBS.
1st Group meeting

3.16.2015

1st Group meeting. Everyone was assigned with his/her own mission. Brainstorm began.
3.15.2015

Visited the AST space of Zhejiang Science&Technology Museum. Decided to collaborate with.
3.13.2015

Concluded the efficiency and fruits of winter project.
Beginning & Winter
2.1.2015-3.13.2015

Winter project began. Everyone read wikis of projects in the past few years.
1.31.2015

1st meetup. Had a nice meal~
1.30.2015

15 members was picked out. ZJU-China 2015 team was set up~ Our story began.

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+<p><small class="text-muted">9.5.2015</small></p>
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+<p>Finished the overview of entire project.
+</p>
+</div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/3/3d/9-5.jpg/800px-9-5.jpg" class="img-responsive">
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+<p><small class="text-muted">9.4.2015</small></p>
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+<ul>
+<li><p class="p1">Bacteria solution PCR: OrfX. One positive clone of 20 samples.</p></li>
+<li><p class="p1">DNA sequencing: OrfX.</p></li>
+</ul>
+</div>
+</div>
+</li>
+ <li class="entry">
+<div class="timeline-panel">
+<div class="tl-heading">
+<p><small class="text-muted">9.3.2015</small></p>
+</div>
+<div class="tl-body">
+<p>Watched the military parade during the commemoration activities to mark the 70th anniversary of the victory of the Chinese People's War of Resistance Against Japanese Aggression and the World Anti-Fascist War in our lab.
+</p>
+</div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/0/0c/9-3.jpg/800px-9-3.jpg" class="img-responsive">
+</div>
+</li>
+ <li class="entry">
+<div class="timeline-panel">
+<div class="tl-heading">
+<p><small class="text-muted">9.2.2015</small></p>
+</div>
+<div class="tl-body">
+<p>Dynamic Light Scattering(DLS) experiment to measure the particle size of embedded E.coli.
+</p>
+</div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/d/d5/9-2.jpg/800px-9-2.jpg" class="img-responsive">
+</div>
+</li>
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+<div class="tl-heading">
+<p><small class="text-muted">9.1.2015</small></p>
+</div>
+<div class="tl-body">
+<p>The sequence of OrfX was not correct because of the wrong template, thus we had to do the related experiments again.
+</p>
+</div>
+</div>
+</li>
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+<div class="timeline-panel">
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+<p><small class="text-muted">8.31.2015</small></p>
+</div>
+<div class="tl-body">
+<p>Took photos and prepared for the banner~</p>
+</div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/8/87/8-31-2.jpg/450px-8-31-2.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/e/e7/8-31.jpg/800px-8-31.jpg" class="img-responsive">
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 <p>Seamless assembly: plasmid+CDS-tcdA1, plasmid+CDS-plu0840. DNA gel extraction: tcdA1. PCR: device-plu0840.</p>
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 <p>37th Group meeting. Built the framework for wiki. Discussed the Syn-bio talks on next Saturday.</p>
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 </ul>
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+<img src="8/16" class="img-responsive">
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 </ul>
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 </ul>
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 </ul>
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+<img src="https://static.igem.org/mediawiki/2015/thumb/3/3b/8-13-2.jpg/450px-8-13-2.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/0/0e/8-13.jpg" class="img-responsive">
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 </ul>
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+<img src="https://static.igem.org/mediawiki/2015/thumb/f/f6/8-10.png/470px-8-10.png" class="img-responsive">
 </div>
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 </ul>
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+<img src="https://static.igem.org/mediawiki/2015/thumb/c/c0/8-9-2.jpg/600px-8-9-2.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/3/3f/8-9.jpg/800px-8-9.jpg" class="img-responsive">
 </div>
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 <p>Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).</p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/3/38/8-8-2.png/800px-8-8-2.png" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/b/bb/8-8-3.jpg/800px-8-8-3.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/8/8f/8-8.jpeg/800px-8-8.jpeg" class="img-responsive">
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 </ul>
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+<img src="https://static.igem.org/mediawiki/2015/thumb/e/ed/8-6.png/800px-8-6.png" class="img-responsive">
 </div>
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 </ul>
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+<img src="https://static.igem.org/mediawiki/2015/thumb/c/c7/8-5.jpg/450px-8-5.jpg" class="img-responsive">
 </div>
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 <p>Completed the One Step Cloning protocol.</p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/e/e1/8-4.png/494px-8-4.png" class="img-responsive">
 </div>
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 <li class="month" id="July"><div class="tldate" style="font-family:"gennifer",Verdana,Sans-serif">July</div></li>
-<li class="entry timeline-inverted">
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 </div>
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 </div>
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 <p>Giant Jamboree registration completed.</p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/e/e5/7-28.png/420px-7-28.png" class="img-responsive">
 </div>
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 </div>
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 </div>
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 <p>Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST. </p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/3/3b/7-19.jpg/800px-7-19.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/b/bc/7-20.jpg/800px-7-20.jpg" class="img-responsive">
 </div>
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 <p>Prepared the home page and member page of our wiki. Prepared the poster for NCTU meetup.</p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/4/42/7-16.png/338px-7-16.png" class="img-responsive">
 </div>
 </li>
-<li class="entry">
+ <li class="entry">
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@@ Line 552: / Line 722: @@
 </ul>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/2/2d/7-15.jpg/480px-7-15.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/5/51/7-15.png" class="img-responsive">
 </div>
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+ <li class="entry">
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@@ Line 564: / Line 738: @@
 </div>
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-<li class="entry timeline-inverted">
+ <li class="entry timeline-inverted">
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 <p>31st Group meeting. Reviewed the medal requirement and all the rules in lab.</p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/e/e8/7-12.png/605px-7-12.png" class="img-responsive">
 </div>
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 </div>
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-<li class="entry timeline-inverted">
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 </div>
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 </div>
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 </div>
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 </div>
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 </div>
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 </div>
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+ <li class="entry">
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 <p>TEM and SEM scanned our samples.</p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/8/8a/6-17-3.png/798px-6-17-3.png" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/7/74/6-17-2.jpg/590px-6-17-2.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/a/a4/6-17.jpg" class="img-responsive">
 </div>
 </li>
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+ <li class="entry">
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 </div>
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@@ Line 714: / Line 908: @@
 <p>27th Group meeting. Rearranged all the events with four quadrant method. Checked everything we have had. </p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/7/70/6-14.jpg/800px-6-14.jpg" class="img-responsive">
 </div>
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-<li class="entry">
+ <li class="entry">
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@@ Line 723: / Line 920: @@
 <div class="tl-body">Solved the diffusion equation.
 </div>
+<img src="https://static.igem.org/mediawiki/2015/3/3d/6-14-2.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/b/ba/6-14-3.png/800px-6-14-3.png" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/2/29/6-14-4.jpg/800px-6-14-4.jpg" class="img-responsive">
 </div>
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@@ Line 735: / Line 937: @@
 </div>
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@@ Line 745: / Line 948: @@
 </div>
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-<li class="entry timeline-inverted">
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 </div>
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 </div>
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@@ Line 786: / Line 993: @@
 <p>Optimization of the model of termites finding food.</p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/8/82/6-8-2.png" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/2/27/6-8-3.png/800px-6-8-3.png" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/1/1b/6-8.png" class="img-responsive">
 </div>
 </li>
-<li class="entry timeline-inverted">
+ <li class="entry timeline-inverted">
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@@ Line 799: / Line 1,011: @@
 </div>
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 <div class="tl-heading">
@@ Line 808: / Line 1,021: @@
 </div>
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@@ Line 816: / Line 1,030: @@
 <p>Frozen the spores of Streptomyces avermitilis.</p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/0/03/6-6.jpeg/800px-6-6.jpeg" class="img-responsive">
 </div>
 </li>
-<li class="entry timeline-inverted">
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@@ Line 827: / Line 1,044: @@
 <p>24th Group meeting. Brainstormed the Synbio-cards and Synbio-workshops. Listed the datailed personal scheme about the time arrangement before summer holiday.</p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/9/98/6-4-2.png/525px-6-4-2.png" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/4/41/6-4.jpg/692px-6-4.jpg" class="img-responsive">
 </div>
 </li>
-<li class="entry">
+ <li class="entry">
 <div class="timeline-panel">
 <div class="tl-heading">
@@ Line 841: / Line 1,062: @@
 </ul>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/8/8d/6-3.png/800px-6-3.png" class="img-responsive">
 </div>
 </li>
-<li class="entry">
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@@ Line 853: / Line 1,077: @@
 </div>
 </li>
-<li class="entry">
+ <li class="entry">
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@@ Line 866: / Line 1,091: @@
 <li class="month" id="May"><div class="tldate" style="font-family:"gennifer",Verdana,Sans-serif">May</div></li>
-<li class="entry timeline-inverted">
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 </div>
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@@ Line 884: / Line 1,111: @@
 <div class="tl-body">Taught high school students to do experiment to extract DNA from epithelial cell of mouth .Built up the model of termites finding food.
 </div>
+<img src="https://static.igem.org/mediawiki/2015/7/7a/5-31-2.png" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/b/b7/5-31-3.jpg/800px-5-31-3.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/f/f5/5-31-2.jpg/800px-5-31-2.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/7/78/5-31.jpg/800px-5-31.jpg" class="img-responsive">
 </div>
 </li>
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@@ Line 901: / Line 1,133: @@
 </div>
 </li>
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@@ Line 911: / Line 1,144: @@
 </div>
 </li>
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@@ Line 921: / Line 1,155: @@
 </div>
 </li>
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@@ Line 931: / Line 1,166: @@
 </div>
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-<li class="entry timeline-inverted">
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@@ Line 942: / Line 1,178: @@
 </div>
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@@ Line 952: / Line 1,189: @@
 </div>
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-<li class="entry timeline-inverted">
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 </div>
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@@ Line 972: / Line 1,211: @@
 </div>
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@@ Line 985: / Line 1,225: @@
 </div>
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@@ Line 993: / Line 1,234: @@
 <p>Detailed the medal requirements depending on our project. Finished the protocol about Streptomyces avermitilis.</p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/8/89/5-19-2.png/800px-5-19-2.png" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/8/89/5-19-2.png/800px-5-19-2.png" class="img-responsive">
 </div>
 </li>
-<li class="entry">
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 <div class="timeline-panel">
 <div class="tl-heading">
@@ Line 1,005: / Line 1,250: @@
 </div>
 </li>
-<li class="entry timeline-inverted">
+ <li class="entry timeline-inverted">
 <div class="timeline-panel">
 <div class="tl-heading">
@@ Line 1,016: / Line 1,262: @@
 </div>
 </li>
-<li class="entry">
+ <li class="entry">
 <div class="timeline-panel">
 <div class="tl-heading">
@@ Line 1,023: / Line 1,270: @@
 <div class="tl-body">Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio.
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/a/a3/5-17.jpg/800px-5-17.jpg" class="img-responsive">
 </div>
 </li>
-<li class="entry">
+ <li class="entry">
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 <div class="tl-heading">
@@ Line 1,033: / Line 1,283: @@
 <p>Read paper and discussed how to enhance the efficiency of Avermectin in Streptomyces avermitilis.</p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/4/4a/5-15.png/800px-5-15.png" class="img-responsive">
 </div>
 </li>
-<li class="entry timeline-inverted">
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@@ Line 1,046: / Line 1,299: @@
 </div>
 </li>
-<li class="entry">
+ <li class="entry">
 <div class="timeline-panel">
 <div class="tl-heading">
@@ Line 1,058: / Line 1,312: @@
 </ul>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/0/06/5-14-2.jpg/590px-5-14-2.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/f/f4/5-14-3.jpg/800px-5-14-3.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/4/48/5-14.jpg/590px-5-14.jpg" class="img-responsive">
 </div>
 </li>
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@@ Line 1,073: / Line 1,332: @@
 </div>
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@@ Line 1,083: / Line 1,343: @@
 </div>
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@@ Line 1,105: / Line 1,367: @@
 </div>
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+ <li class="entry">
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 <div class="tl-heading">
@@ Line 1,116: / Line 1,379: @@
 </ul>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/c/c1/5-6.jpg/800px-5-6.jpg" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/e/e4/5-6-2.jpg/800px-5-6-2.jpg" class="img-responsive">
 </div>
 </li>
-<li class="entry">
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@@ Line 1,128: / Line 1,394: @@
 </div>
 </li>
-<li class="entry">
+ <li class="entry">
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@@ Line 1,141: / Line 1,408: @@
 <li class="month" id="April"><div class="tldate" style="font-family:"gennifer",Verdana,Sans-serif">April</div></li>
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-<p>14th Group meeting. 10 members would go to Taiwan for NCTU meetup. Results of consulting professors specialized in termites and Bacillus subtilis.
+<p>14th Group meeting. 10 members would go to Taiwan for NCTU meetup. Results of consulting professors specialized in termites and Bacillus subtilis.</p>
-</p>
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 <p>12th Group meeting. Debugged the previous package plan and decided to pack bacterium with CNC. Planned to buy a nest of termites from Guangdong Province. Discussed several demensions of modeling in this project.</p>
 </div>
+<img src="https://static.igem.org/mediawiki/2015/thumb/a/a0/4-23-2.jpg/600px-4-23-2.jpg" class="img-responsive">
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 <p>Consulted professors about directly embedding baterium with CNC rather than with the aid of protein domain. Our idea was confirmed and praised.</p>
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 <p>Communication with our instructor. Searched for information about cellulose binding domain(CBDs) and overexpression of ivermectin and avermectin.</p>
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+<img src="https://static.igem.org/mediawiki/2015/thumb/1/18/4-20.png/553px-4-20.png" class="img-responsive">
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 <p>11th Group meeting. Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project. </p>
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 <p>Preliminarily designed the circuit in Streptomyces avermitilis.</p>
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 <p>9th Group meeting. Kept 3 ideas and the final idea would derive from them. Detailed these 3 ideas from all the aspects.</p>
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 <p>7th Group meeting. Began to kill some undoable ideas and further dug the existing ideas, such as biological enigma machine and killing termites.</p>
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+<img src="https://static.igem.org/mediawiki/2015/1/10/4-6-2.png" class="img-responsive">
+<img src="https://static.igem.org/mediawiki/2015/thumb/c/cf/4-6.png/800px-4-6.png" class="img-responsive">
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 <p>6th Group meeting. Developed several new ideas, including bio-SCM, biological enigma machine and slow release of certain chemical. Talked about their feasibility.</p>
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-<h4>3rd Group meeting</h4>
+<p><small class="text-muted">3.25.2015</small></p>
-<p><small class="text-muted">3.22.2015</small></p>
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-<p>3rd Group meeting. Discussed some ideas about modeling. Shared new ideas.</p>
+<p>Team application completed.</p>
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-<p><small class="text-muted">3.25.2015</small></p>
+<h4>3rd Group meeting</h4>
+<p><small class="text-muted">3.22.2015</small></p>
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-<p>Team application completed.</p>
+<p>3rd Group meeting. Discussed some ideas about modeling. Shared new ideas.</p>
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 <p>Concluded the efficiency and fruits of winter project.</p>
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 <li class="month" id="Beginning"><div class="tldate" style="font-family:"gennifer",Verdana,Sans-serif">Beginning & Winter</div></li>
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 <p>Winter project began. Everyone read wikis of projects in the past few years.</p>
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 <p>1st meetup. Had a nice meal~</p>
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 <p>15 members was picked out. ZJU-China 2015 team was set up~ Our story began.</p>
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Difference between revisions of "Team:ZJU-China/Notebook"

Revision as of 06:37, 8 September 2015

Timeline

38th Group meeting

37th Group meeting

35th Group meeting

34th Group meeting

33rd Group meeting

32nd Group meeting

31st Group meeting

30th Group meeting

29th Group meeting

28th Group meeting

27th Group meeting

26th Group meeting

25th Group meeting

24th Group meeting

23rd Group meeting

21st Group meeting

18th Group meeting

17th Group meeting

16th Group meeting

15th Group meeting

14th Group meeting

13th Group meeting

12th Group meeting

11th Group meeting

10th Group meeting

9th Group meeting

8th Group meeting

7th Group meeting

6th Group meeting

5th Group meeting

4th Group meeting

3rd Group meeting

2nd Group meeting

1st Group meeting