Difference between revisions of "Team:CHINA CD UESTC/Modeling"
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− | Biological fuel cell (BFC) is a device with the use of enzymes or microorganisms as catalysts. According to the types of catalysts, it can be divided into microbial fuel cells(MFC)and enzymatic biofuel cell (EBFC). Our project is focused on improving the catalytic efficiency of laccase in biofuel cell. Laccase, produced by advanced plants, fungi, and some bacterial strains, is a multicopper oxidoreductase and oxidizes phenolic compounds while reducing oxygen to water directly without requiring H<sub>2</sub>O<sub>2</sub> | + | Biological fuel cell (BFC) is a device with the use of enzymes or microorganisms as catalysts. According to the types of catalysts, it can be divided into microbial fuel cells(MFC)and enzymatic biofuel cell (EBFC). Our project is focused on improving the catalytic efficiency of laccase in biofuel cell. Laccase, which produced by advanced plants, fungi, and some bacterial strains, is a multicopper oxidoreductase and oxidizes phenolic compounds while reducing oxygen to water directly without requiring H<sub>2</sub>O<sub>2</sub> |
or any other co-factors for its catalysis <sup>[1]</sup> | or any other co-factors for its catalysis <sup>[1]</sup> | ||
. In addition to be applied in the biological fuel cells, it also has various applications containing pulp and paper industry, environmental applications, food industry, and biosensors. | . In addition to be applied in the biological fuel cells, it also has various applications containing pulp and paper industry, environmental applications, food industry, and biosensors. | ||
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− | One | + | One criteria of evaluating a microbial fuel cell is potential. We use the laccase as a bio-cathode to achieve electron transfer function from pole to oxygen. |
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<img src="https://static.igem.org/mediawiki/2015/e/ee/CHINA_CD_UESTC_MODELING04.png" width="60%"> | <img src="https://static.igem.org/mediawiki/2015/e/ee/CHINA_CD_UESTC_MODELING04.png" width="60%"> | ||
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− | Figure 1:With the increase of electron transfer coefficient, cathodic peak potential is also increasing rapidly. But the value | + | Figure 1:With the increase of electron transfer coefficient, cathodic peak potential is also increasing rapidly. But the value of α is form 0.3 to 0.7 in general. |
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− | Form figure 1, we learn about the electron transfer coefficient determines the potential. So what determines the electron transfer coefficient ? We think enzyme quantity per unit area and the distance between enzymes and electrodes are the main influence factors. So we establish the formula: | + | Form figure 1, we learn about the electron transfer coefficient determines the potential. So what determines the electron transfer coefficient? We think enzyme quantity per unit area and the distance between enzymes and electrodes are the main influence factors. So we establish the formula: |
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− | There are many methods to fix laccase on the electrode. | + | There are many methods to fix laccase on the electrode. However, we came up with a novel idea utilizing biology magnetotaxis. Magnetotactic bacterium(MTB) is a special microbe in nature which can be attracted by magnet . Magnetosome, covered by membrane with some Fe3O4s nanocrystals, which is the reason why MTB has the ability to be attracted. We design a expression system for <i>E.coli</i> expressing Magnetosomes. And then we link laccase to Magnetosomes by the method of structuring fusion protein with MamW. The ultimate goal is showing in the figure4: |
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Revision as of 07:14, 9 September 2015
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MODELING
In order to predict the potential of enzyme biofuel cell, analyze in hypothesis condition, speculate the efficiency of laccase’s catalytic, calculate the electron transfer coefficient and verify our formula, we have established a number of models and conducted a series of calculations. The following page will show you the details.