• Background
• Methods
• Results
• Discussion
• Future work

RESULTS

2. Enzyme and protein assays

In order to make a better expression of the protein in the cell, we decided to make a IPTG gradient induction for our gene circuits after the reference. Here ares our rusults.

Figure 2.1 SDS-PAGE of purified LeuDH (34+L) from DE3

Figure 2.2 Different concentrations of IPTG on the Enzyme activity of 34+L

Figure 2.3 SDS-PAGE of purified FDH(34+F) from DE3

Figure 2.4 Different concentrations of IPTG on the Enzyme activity of 34+F

Figure 2.5 SDS-PAGE of purified LeuDH (30+L)from DE3

Figure 2.6 Different concentrations of IPTG on the Enzyme activity of 30+L

Figure 2.7 SDS-PAGE of purified LeuDH(30+L) and FDH(34+F) from DE3

Figure 2.8 Different concentrations of IPTG on the Enzyme activity of 30+L+34+

Figure 2.9 SDS-PAGE of purified LeuDH (34+L)and FDH (34+L)from DE3

Figure 2.10 Different concentrations of IPTG on the Enzyme activity of 34+L+34+F

Figure 2.11 SDS-PAGE of purified LeuDH(32+L) and FDH(34+F) from DE3

Figure 2.12 Different concentrations of IPTG on the Enzyme activity of 32+L+34+F

From the enzyme activity data, we obtained the most suitable IPTG concentration of different circuits, as follows.

When we get the most appropriate concentration, we start with High Performance Liquid Chromatography