Difference between revisions of "Team:UiOslo Norway/Experiments/Protein Expression"

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Revision as of 11:04, 9 September 2015

Protein Expression:

Back to Protocols


  • Inoculate 5 ml preculture and incubate at 37 °C overnight shaking at 220 rpm

  • Measure OD600 and calculate the amount needed to inoculate an expression culture with and OD600 of 0.3

  • Inoculate the expression culture and incubate at 37 °C for 1 hour shaking at 220 rpm

  • Determine OD600

  • Recommended is an OD600 between 0.4-1.0 to induce protein expression

  • Take a 1 ml sample in an uninduced stage

  • Induce protein expression by adding IPTG to a final concentration of 1 mM

  • Incubate the culture for 3 hours at 37 °C shaking at 220 rpm

  • Harvest cells by centrifugation at 8,000 x g for 5 minutes


    • iGEM UiOslo 2015 was sponsored by: