Difference between revisions of "Team:UiOslo Norway/Experiments/Solubility Assay"

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Revision as of 11:04, 9 September 2015

Solubility Assay:

Back to Protocols


  • Resuspend cell pellet in 600 ul PBS buffer (pH 7.4)

  • Add glass beads and disrupt cells by vortexing

  • Centrifuge at 16.000 x g for 5 minutes

  • Transfer soluble fraction into a new tube and resuspend insoluble fraction in 600 ul PBS buffer (pH 7.4)


    • iGEM UiOslo 2015 was sponsored by: