Difference between revisions of "Team:Bordeaux/Results"

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<h4>Why choosing crdS, crdA and crdC genes ?</h4>
 
<h4>Why choosing crdS, crdA and crdC genes ?</h4>
  
&#10037; <b>crdS gene</b> codes the Curdlan synthase.
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<p align=justify>&#10037; <b>crdS gene</b> codes the Curdlan synthase.
&#10037; crdA gene</b> codes a protein which assists translocation of nascent polymer across cytoplasmic membrane.  
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<br>&#10037; <b>crdA gene</b> codes a protein which assists translocation of nascent polymer across cytoplasmic membrane.  
&#10037; crdC gene</b> codes a protein which assists translocation of nascent polymer across the periplasm.  
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<br>&#10037; <b>crdC gene</b> codes a protein which assists translocation of nascent polymer across the periplasm.
 
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<p align="justify"> crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in Agrobacterium sp.ATCC31749.</p>
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<br>crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in ''Agrobacterium sp.''ATCC31749.</p>
  
 
<span style="border:1px solid black;padding:1%">We tried to work on these three genes. However, amplification attempts by PCR were unsuccessful for crdA and crdC genes.
 
<span style="border:1px solid black;padding:1%">We tried to work on these three genes. However, amplification attempts by PCR were unsuccessful for crdA and crdC genes.

Revision as of 13:37, 9 September 2015

IGEM Bordeaux 2015

Project Results

Why choosing crdS, crdA and crdC genes ?

crdS gene codes the Curdlan synthase.
crdA gene codes a protein which assists translocation of nascent polymer across cytoplasmic membrane.
crdC gene codes a protein which assists translocation of nascent polymer across the periplasm.

crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in ''Agrobacterium sp.''ATCC31749.

We tried to work on these three genes. However, amplification attempts by PCR were unsuccessful for crdA and crdC genes. So, in a first time, we focused on cloning crdS gene only.


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