Difference between revisions of "Team:Bordeaux/Results"

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<h3> Project Results</h3>
 
<h3> Project Results</h3>
  
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<h4>Why choosing crdS, crdA and crdC genes ?</h4>
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<h5><align="justify">Why choosing crdS, crdA and crdC genes ?</align></h5>
  
 
<p align=justify>&#10037; <b>crdS gene</b> codes the Curdlan synthase.
 
<p align=justify>&#10037; <b>crdS gene</b> codes the Curdlan synthase.
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<br>&#10037; <b>crdC gene</b> codes a protein which assists translocation of nascent polymer across the periplasm.
 
<br>&#10037; <b>crdC gene</b> codes a protein which assists translocation of nascent polymer across the periplasm.
 
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<br>crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in ''Agrobacterium sp.''ATCC31749.</p>
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<br>crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in <i>Agrobacterium sp.</i> ATCC31749.</p>
  
 
<span style="border:1px solid black;padding:1%">We tried to work on these three genes. However, amplification attempts by PCR were unsuccessful for crdA and crdC genes.
 
<span style="border:1px solid black;padding:1%">We tried to work on these three genes. However, amplification attempts by PCR were unsuccessful for crdA and crdC genes.

Revision as of 13:40, 9 September 2015

IGEM Bordeaux 2015

Project Results

Why choosing crdS, crdA and crdC genes ?

✵ crdS gene codes the Curdlan synthase.
✵ crdA gene codes a protein which assists translocation of nascent polymer across cytoplasmic membrane.
✵ crdC gene codes a protein which assists translocation of nascent polymer across the periplasm.

crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in Agrobacterium sp. ATCC31749.

We tried to work on these three genes. However, amplification attempts by PCR were unsuccessful for crdA and crdC genes. So, in a first time, we focused on cloning crdS gene only.


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