Difference between revisions of "Team:Bordeaux/Results"

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<br>&#10037; <b>crdA gene</b> codes a protein which assists translocation of nascent polymer across cytoplasmic membrane.  
 
<br>&#10037; <b>crdA gene</b> codes a protein which assists translocation of nascent polymer across cytoplasmic membrane.  
 
<br>&#10037; <b>crdC gene</b> codes a protein which assists translocation of nascent polymer across the periplasm.
 
<br>&#10037; <b>crdC gene</b> codes a protein which assists translocation of nascent polymer across the periplasm.
<br>
 
 
<br>crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in <i>Agrobacterium sp. ATCC31749</i>.</p>
 
<br>crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in <i>Agrobacterium sp. ATCC31749</i>.</p>
  
<span style="border:1px solid black;padding:1%">We tried to work on these three genes.  
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<span style="border:1px solid black;padding:1%">We tried to work on these three genes. However, amplification attempts by PCR were unsuccessful for crdA and crdC genes.So, in a first time, we focused on cloning crdS gene only.</span>
<br>However, amplification attempts by PCR were unsuccessful for crdA and crdC genes.
+
<br>So, in a first time, we focused on cloning crdS gene only.<span>
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       </div>
 
       </div>

Revision as of 13:52, 9 September 2015

IGEM Bordeaux 2015

Project Results

Why choosing crdS, crdA and crdC genes ?

crdS gene codes the Curdlan synthase.
crdA gene codes a protein which assists translocation of nascent polymer across cytoplasmic membrane.
crdC gene codes a protein which assists translocation of nascent polymer across the periplasm.
crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in Agrobacterium sp. ATCC31749.

We tried to work on these three genes. However, amplification attempts by PCR were unsuccessful for crdA and crdC genes.So, in a first time, we focused on cloning crdS gene only.



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