Difference between revisions of "Team:Bordeaux/Results"
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| − | <h5 align="left" > Why choosing crdS, crdA and crdC genes ?</h5> | + | <!-- CHOOSING PARTS ------------------------------------------------------------------------------------------- --> |
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| + | <h5 align="left"> Why choosing crdS, crdA and crdC genes ?</h5> | ||
<p align=justify>✵ <b>crdS gene</b> codes the Curdlan synthase. | <p align=justify>✵ <b>crdS gene</b> codes the Curdlan synthase. | ||
<br>✵ <b>crdA gene</b> codes a protein which assists translocation of nascent polymer across cytoplasmic membrane. | <br>✵ <b>crdA gene</b> codes a protein which assists translocation of nascent polymer across cytoplasmic membrane. | ||
<br>✵ <b>crdC gene</b> codes a protein which assists translocation of nascent polymer across the periplasm. | <br>✵ <b>crdC gene</b> codes a protein which assists translocation of nascent polymer across the periplasm. | ||
| − | <br>crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in <i>Agrobacterium sp. ATCC31749</i>.</p> | + | <br>crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in <i>Agrobacterium sp. ATCC31749</i>. |
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| + | <br>N.B : We tried to work on these three genes. However, amplification attempts by PCR were unsuccessful for crdA and crdC genes.So, in a first time, we focused on cloning crdS gene only.</p> | ||
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| + | <h5 align="left"> Why choosing OsmY promoter ?</h5> | ||
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| + | <align="justify">In <i>Agrobacterium sp.ATCC31749</i>, Curdlan production is started after a nitrogen starvation in stationary phase. So we decided to use OsmY promoter (BBa_J45992) characterized by MIT 2006 iGEM team which is active in stationary phase and under high osmotic pressure condition. This promoter imitates Curdlan biosynthesis in E.coli without the nitrogen stress.</align> | ||
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| + | <h5 align="left">Why choosing M63 and LB medium</h5> | ||
| − | < | + | <align="justify">Curdlan production was carried out in two different media: LB medium and M63 medium. |
| + | <br>✵ We worked on M63 medium because this one is cited in literature. M63 is a minimal, low osmolarity medium for E.coli, resulting in slower growth rate of these cells. | ||
| + | XXX Mettre la reference de la publication XXX | ||
| + | →With this medium of known composition we were able to control parameters for the production of our molecule of interest. | ||
| + | <br>✵We worked also on LB medium because this is the most common medium used in the laboratory.</align> | ||
</div> | </div> | ||
Revision as of 14:03, 9 September 2015