Difference between revisions of "Team:UiOslo Norway/Experiments/Dialysis"

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<p> coming soon </p>
 
  
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<h1>Dialysis</h1>
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<a href="https://2015.igem.org/Team:UiOslo_Norway/Experiments" >
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Back to Protocols
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</a>
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<p></br></p>
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<p>
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<ul>
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  <li><p>Combine the elution fractions that are pure and containing the protein of interest.</p></li>
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  <li><p>Dialyse against dialysis buffer for 2 hours and change the buffer after 1 hour.</p></li>
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<p><b>Dialysis buffer</b></br>
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</br>
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20 mM Tris-HCl pH 8 </br>
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50 mM NaCl</br>
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0.5 mM  EDTA</br>
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10 mM  beta-Mercaptoethanol</br>
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</br>
 
</html>
 
</html>

Revision as of 11:59, 10 September 2015

Dialysis

Back to Protocols


  • Combine the elution fractions that are pure and containing the protein of interest.

  • Dialyse against dialysis buffer for 2 hours and change the buffer after 1 hour.

    • Dialysis buffer

    20 mM Tris-HCl pH 8
    50 mM NaCl
    0.5 mM EDTA
    10 mM beta-Mercaptoethanol