Difference between revisions of "Team:Valencia UPV/Notebook"

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<h3 style="color:green">5 June 2015</h3>
  
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<p>We had 2 cultures from the last day, corresponding to other 2 colonies of ligation. </p>
  
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<p><i>Agrobacterium</i> culture of promoter less: Luciferase + Renilla </p>
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<p>Minipreps</p>
  
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<p>Digestion with BamHI and EcoRV</p>
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<tr><td>PIF6 + PhyB; &Omega;1</td><td>Etr8 CMV+Bxb1_T35S; &alpha;1</td></tr>
  
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<tr><td>1µL (GB892) PIF; &alpha;1</td><td>1µL 1097 (Etr8 CMV) pUPD2</td></tr>
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<tr><td>1µL (GB88E) PhyB; &alpha;2</td><td>1µL Bxb1; pUPD2</td></tr>
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Revision as of 13:12, 10 September 2015

Valencia UPV iGEM 2015

Notebook

5 June 2015

We had 2 cultures from the last day, corresponding to other 2 colonies of ligation.

Agrobacterium culture of promoter less: Luciferase + Renilla

Minipreps

Digestion with BamHI and EcoRV

PIF6 + PhyB; Ω1Etr8 CMV+Bxb1_T35S; α1
1µL (GB892) PIF; α11µL 1097 (Etr8 CMV) pUPD2
1µL (GB88E) PhyB; α21µL Bxb1; pUPD2
1µL Ω1 1µL Tnos PuPD
1.2µL Buffer ligase1µL α1
1µL Bsmb15.8µL H2O
6.8µL H2O