Difference between revisions of "Team:TU Dresden/Notebook/Protocols/AgaroseGel"

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<p style="line-height:1.8;><b>Wear nitrile gloves when preparing the gel, ethidium bromide is considered toxic. </b></p>
 
<p style="line-height:1.8;><b>Wear nitrile gloves when preparing the gel, ethidium bromide is considered toxic. </b></p>
  
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Recipe for 10xTBE buffer:
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<p style="line-height:1.8;>Recipe for 10x TBE buffer:</p>
 +
<ul>
 +
  <li style="margin-bottom: 10px;line-height:1.8;">0.89 M Boric acid (M<sub>r</sub> 121.14).</li>
 +
  <li style="margin-bottom: 10px;line-height:1.8;"></li>
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  <li style="margin-bottom: 10px;line-height:1.8;"></li>
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</ul>
  
 
0.89 M Boric acid,      Mr 121.14
 
0.89 M Boric acid,      Mr 121.14

Revision as of 13:43, 10 September 2015


How to prepare a 0.7 % agarose gel

Recipe for 10x TBE buffer:

  • 0.89 M Boric acid (Mr 121.14).
0.89 M Boric acid, Mr 121.14 0.89 M Tris, Mr 61.83 0.02 M EDTA pH 8.0 500 mM stock solution pH 8.0
  3. μL °C