Difference between revisions of "Team:NAIT Edmonton/Protocols"

Line 756: Line 756:
  
 
         <center><h1>Digestion of Vectors and Inserts</h1></center><br><br>
 
         <center><h1>Digestion of Vectors and Inserts</h1></center><br><br>
       <center>Two enzymes are needed in order to cut the DNA stands at specific restriction sites</center>
+
       Two enzymes are needed in order to cut the DNA stands at specific restriction sites<br>
         <center>1. Find the protocol of restriction enzymes being used</center>
+
         1. Find the protocol of restriction enzymes being used<br>
         <center>Use the </center>
+
         Use the <br>
 
<center></center>
 
<center></center>
 
<center></center>
 
<center></center>

Revision as of 14:16, 10 September 2015

Team NAIT 2015

Experimental Design

Go through our interactive experimental design flow chart! Many of our protocols are manufacturer specicfied; however, some are customized by us! Click on the flow chart boxes if the hand cursor appears to read more about our customized protocols.

PDFs of protocols can also be found

Theorizing our Sequences
Literature has shown certain proteins inherently stain in colour.
Looked up characteristics of said proteins
Isolated and identified the unique characteristics of said proteins so that we can manually write our own sequences and generate custom proteins.
Writing our Sequences
Went down to base pair level and wrote out our sequences with the defining characteristics identified in literature.
PCR
Digestion and Ligation
Transforming Bacteria
Validating the Transformation
Protein Isolation and Purification
SDS PAGE and Silver Staining