Difference between revisions of "Team:Shiyan SY China/Parts"

Line 317: Line 317:
 
                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Project.html';"title="">PROJECT</a></h3>
 
                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Project.html';"title="">PROJECT</a></h3>
 
                             <ul class="sub">
 
                             <ul class="sub">
−
                        <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t1">Background</a> </li>
+
                        <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t1">Description</a> </li>
−
                             <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t2">Design</a> </li>
+
                        <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t2">Background</a> </li>
−
                             <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t3">Results</a> </li>
+
                             <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t3">Design</a> </li>
−
                             <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t4">Parts</a> </li>
+
                             <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t4">Experiment&Steps</a> </li>
 +
                            <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t5">Results&Future</a> </li>
 +
                             <li ><a style ="background:#27AE60" href="https://2015.igem.org/Team:Shiyan_SY_China/Project.html#t6">Parts</a> </li>
 
    </ul>
 
    </ul>
  
Line 341: Line 343:
 
                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Safety.html';" title="">SAFETY</a></h3>
 
                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Safety.html';" title="">SAFETY</a></h3>
 
                             <ul class="sub">
 
                             <ul class="sub">
−
                        <li ><a style ="background:#0498F9" href="https://2015.igem.org/Team:Shiyan_SY_China/Safety.html">Safety</a> </li>
+
                        <li ><a style ="background:#BE382A" href="https://2015.igem.org/Team:Shiyan_SY_China/Safety.html#t1">Safety Design</a> </li>
 +
                        <li ><a style ="background:#BE382A" href="https://2015.igem.org/Team:Shiyan_SY_China/Safety.html#t2">Lab security</a> </li>
 +
                        <li ><a style ="background:#BE382A" href="https://2015.igem.org/Team:Shiyan_SY_China/Safety.html#t3">Safety Delivery</a> </li>
 
                             </ul>
 
                             </ul>
 
                         </li>
 
                         </li>
Line 356: Line 360:
 
                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Notebook.html';"  title="">NOTEBOOK</a></h3>
 
                             <h3><a target="_blank" style="cursor: pointer;" onclick="location.href ='https://2015.igem.org/Team:Shiyan_SY_China/Notebook.html';"  title="">NOTEBOOK</a></h3>
 
                             <ul class="sub">
 
                             <ul class="sub">
−
                        <li ><a style ="background:#BE382A" href="https://2015.igem.org/Team:Shiyan_SY_China/Notebook.html#t1">Notebook</a> </li>
+
                        <li ><a style ="background:#0498F9" href="https://2015.igem.org/Team:Shiyan_SY_China/Notebook.html#t1">Notebook</a> </li>
−
                            <li ><a style ="background:#BE382A" href="https://2015.igem.org/Team:Shiyan_SY_China/Notebook.html#t2">Lab Picture</a> </li>
+
 
 
                             </ul>
 
                             </ul>
 
                         </li>
 
                         </li>
Line 382: Line 386:
 
<h style="font-size:15px;font-weight: bold;"> Our submission parts: </h></br>
 
<h style="font-size:15px;font-weight: bold;"> Our submission parts: </h></br>
 
<div>&nbsp;</div>
 
<div>&nbsp;</div>
−
<h style="font-size:15px;font-weight: bold;"> BBa_B0010 </h>
+
<h style="font-size:15px;font-weight: bold;"> BBa_K1667005</h>
  
 
<p>
 
<p>
−
&nbsp;&nbsp;The strong promoter has a strong function to start the downstream RNA transcription, and the transcription of the downstream opdA gene promoter is regulated by the promoter. The RNA structure of the thermometer RNA, which is formed by the RNA structure of the sequence, is formed at the temperature below 32. The structure of the RNA is blocked by the RNA. OmpA signal peptide can lead to opdA protein molecules secreted by the downstream expression of the host cell wall, and in the cell wall to form a opdA enzyme with the degradation of organic phosphorus pesticides, the enzyme can be hydrolyzed in the environment of organic phosphorus pesticides, pesticide toxicity.
+
This part includes two individual DNA domains. 1. A strong promoter followed with a RNA thermometer sequence, which regulate the transcription of opdA gene and expression of opdA protein out of the host cell via an ompA peptide. This DNA fragment domain could be obtained by double-digested with EcoR I + Spe I. 2. A UV induced promoter (RecA SOS) followed with a suicide gene ccdB, which could lead to the death of host cell if exposed in UV. This DNA fragment domain could be obtained by double-digested with Xho I + Spe I. And the whole part DNA fragment could be obtained by double-digested with Xho I + EcoR I.  
 
</p>
 
</p>
 
<div>&nbsp;</div>
 
<div>&nbsp;</div>
Line 415: Line 419:
 
<p>© 2015 Shiyan_SY_China iGEM Team. All rights reserved.  
 
<p>© 2015 Shiyan_SY_China iGEM Team. All rights reserved.  
 
</p>
 
</p>
 +
<div id="ToTop" title="To Top" style="position:fixed;bottom:120px;right:60px;display:none;cursor: pointer;"><img  src="https://static.igem.org/mediawiki/2015/4/4e/Totop.png"  width="40" height="40"  onclick="location.href='#top'";>
 +
</div>
 
</div>
 
</div>
 
</body>
 
</body>
 
</html>
 
</html>

Revision as of 04:01, 11 September 2015

IGEM-PROJECT

You have to believe in yourself. That’s the secret of success.

—— Charles Chaplin

Parts
Our submission parts:
 
BBa_K1667005

This part includes two individual DNA domains. 1. A strong promoter followed with a RNA thermometer sequence, which regulate the transcription of opdA gene and expression of opdA protein out of the host cell via an ompA peptide. This DNA fragment domain could be obtained by double-digested with EcoR I + Spe I. 2. A UV induced promoter (RecA SOS) followed with a suicide gene ccdB, which could lead to the death of host cell if exposed in UV. This DNA fragment domain could be obtained by double-digested with Xho I + Spe I. And the whole part DNA fragment could be obtained by double-digested with Xho I + EcoR I.

 
More information please see our parts form:
 
Name Type Description Length
BBa_K1667005 DNA OpdA encoding gene with ompA signal peptide 1776

© 2015 Shiyan_SY_China iGEM Team. All rights reserved.