Difference between revisions of "Team:NCTU Formosa/Notebook"

Line 558: Line 558:
 
                         </td>
 
                         </td>
 
<td >
 
<td >
−
                             <B>15</B><br><br>Transformation of GBP[FadL-GBP]<br>
+
                             <B>15</B><br><br>
−
Cultivation of OmpA[OmpA+pSB1K3] K<sup>r</sup>
+
Transformation of GBP[FadL-GBP]<br>
 +
Cultivation ofOmpA[OmpA+pSB1K3] K<sup>r</sup>
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
 
                             <B>16</B><br><br>
 
                             <B>16</B><br><br>
 +
Cultivation of GBP[FadL-GBP]
 +
<br>
 +
Plasmid purification of OmpA[OmpA+pSB1K3] K<sup>r</sup>
 +
<br>
 +
Digestion of OmpA[OmpA+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
 +
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
−
                             <B>17</B><br><br>
+
                             <B>17</B><br><br>Plasmid purification of
 +
GBP[FadL-GBP]
 +
<br>
 +
Digestion of
 +
GBP[FadL-GBP]  (EcoRI and PstI)
 +
<br>
 +
Electrophoresis of digested product:
 +
GBP[FadL-GBP]  (EcoRI and PstI)
 +
 
 
                         </td>
 
                         </td>
 
<td >
 
<td >
 
                           <B>18</B><br><br>
 
                           <B>18</B><br><br>
 +
  Transformation of ligated product:
 +
GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
 
                         </td>
 
                         </td>
 
                     </tr>
 
                     </tr>
Line 574: Line 592:
 
                         <td >
 
                         <td >
 
                             <B>19</B><br><br>
 
                             <B>19</B><br><br>
 +
Cultivation of
 +
ligated product: GBP[FadL-GBP] C<sup>r</sup>
 +
PCR product: Terminator[J61048]
 +
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
Line 580: Line 602:
 
                         <td>
 
                         <td>
 
                             <B>21</B><br><br>
 
                             <B>21</B><br><br>
 +
Cultivation of
 +
GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 +
OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
 +
 
                         </td>
 
                         </td>
 
<td >
 
<td >
 
                             <B>22</B><br><br>
 
                             <B>22</B><br><br>
 +
Plasmid purification of
 +
GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 +
OmpA[OmpA+pSB1C3] C<sup>r</sup>
 +
GBP[FadL-GBP+PSB1C3] C<sup>r</sup>
 +
 +
Digestion of
 +
GFP[J23101+B0034+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 +
OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
GBP[FadL-GBP] C<sup>r</sup> (EcoRI and PstI)
 +
 +
Electrophoresis of digested product:
 +
GFP[J23101+B0034+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 +
OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 +
GBP[FadL-GBP] C<sup>r</sup> (EcoRI and PstI)
 +
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
 
                             <B>23</B><br><br>
 
                             <B>23</B><br><br>
 +
Digestion of
 +
OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 +
GBP[FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
 +
 +
Electrophoresis of digested product:
 +
OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 +
GBP[FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
 +
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
 
                             <B>24</B><br><br>
 
                             <B>24</B><br><br>
 +
Digestion of
 +
OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
 +
GBP[FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 +
 +
 
                         </td>
 
                         </td>
 
<td >
 
<td >
 
                             <B>25</B><br><br>
 
                             <B>25</B><br><br>
 +
Electrophoresis of digested product:
 +
OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
 +
GBP[FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 +
 +
Digestion of
 +
RBS[B0034] A<sup>r</sup> (XbaI and PstI)
 +
backbone[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 +
 +
Electrophoresis of digested product:
 +
RBS[B0034] A<sup>r</sup> (XbaI and PstI)
 +
backbone[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 +
Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 +
 +
Ligation of
 +
OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
GBP[GBP+B0034+pSB1K3] K<sup>r</sup>
 +
OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 +
 
                         </td>
 
                         </td>
 
                     </tr>
 
                     </tr>
Line 597: Line 671:
 
                         <td >
 
                         <td >
 
                             <B>26</B><br><br>
 
                             <B>26</B><br><br>
 +
Ligation of
 +
RFP[E1010+B0034+pSB1K3] K<sup>r</sup>
 +
 +
Transformation of ligated product:
 +
OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
GBP[FadL-GBP+B0034+pSB1K3] K<sup>r</sup>
 +
OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 +
 +
Electrophoresis of digested product:
 +
RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 +
[J23101+B0034]
 +
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
 
                           <B>27</B><br><br>
 
                           <B>27</B><br><br>
 +
  Digestion of
 +
Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 +
 +
PCR of ligated product
 +
OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 +
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
 
                             <B>28</B><br><br>
 
                             <B>28</B><br><br>
 +
Electrophoresis of
 +
RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 +
OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 +
 +
Transformation of ligated product:
 +
GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 +
OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 +
 +
PCR of ligated product
 +
OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 
                         </td>
 
                         </td>
 
<td >
 
<td >
 
                             <B>29</B><br><br>
 
                             <B>29</B><br><br>
 +
Cultivation of
 +
RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 +
 +
PCR of
 +
OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 +
Electrophoresis of
 +
OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 +
 +
Ligation of
 +
GBP[FadL-GBP+J61048+pSB1K3] K<sup>r</sup>
 +
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>
 
                             <B>30</B><br><br>
 
                             <B>30</B><br><br>
 +
  Plasmid purification of
 +
RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 +
 +
Ligation of
 +
OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 +
PCR of
 +
OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 +
Transformation of
 +
mini: Terminator[J61048]
 +
ligated product: OmpA[J23101+B0034+OmpA] A<sup>r</sup>
 +
 
                         </td>
 
                         </td>
 
                         <td>
 
                         <td>

Revision as of 07:28, 11 September 2015

Lab note
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

PCR
transform 		2

 3

 4

 5

 6

 7
8

 8

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 11

 12

 13

 14
15

 16

 17

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29

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 31
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

 2

 3

 4
5

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 11
12

 13

 14

 15

Transformation of GBP[FadL-GBP]
Cultivation ofOmpA[OmpA+pSB1K3] Kr 		16

Cultivation of GBP[FadL-GBP]
Plasmid purification of OmpA[OmpA+pSB1K3] Kr
Digestion of OmpA[OmpA+pSB1K3] Kr (EcoRI and PstI) 		17

Plasmid purification of GBP[FadL-GBP]
Digestion of GBP[FadL-GBP] (EcoRI and PstI)
Electrophoresis of digested product: GBP[FadL-GBP] (EcoRI and PstI) 		18

Transformation of ligated product: GBP[FadL-GBP+pSB1C3] Cr
19

Cultivation of ligated product: GBP[FadL-GBP] Cr PCR product: Terminator[J61048] 		20

 21

Cultivation of GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar OmpA[OmpA+pSB1C3] Cr GBP[FadL-GBP+pSB1C3] Cr 		22

Plasmid purification of GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar OmpA[OmpA+pSB1C3] Cr GBP[FadL-GBP+PSB1C3] Cr Digestion of GFP[J23101+B0034+E0040+J61048] Ar (EcoRI and PstI) OmpA[OmpA] Cr (EcoRI and PstI) GBP[FadL-GBP] Cr (EcoRI and PstI) Electrophoresis of digested product: GFP[J23101+B0034+E0040+J61048] Ar (EcoRI and PstI) OmpA[OmpA] Cr (EcoRI and PstI) GBP[FadL-GBP] Cr (EcoRI and PstI) 		23

Digestion of OmpA[OmpA] Cr (EcoRI and SpeI) GBP[FadL-GBP] Cr (EcoRI and SpeI) Electrophoresis of digested product: OmpA[OmpA] Cr (EcoRI and SpeI) GBP[FadL-GBP] Cr (EcoRI and SpeI) 		24

Digestion of OmpA[OmpA] Cr (XbaI and PstI) GBP[FadL-GBP] Cr (XbaI and PstI) 		25

Electrophoresis of digested product: OmpA[OmpA] Cr (XbaI and PstI) GBP[FadL-GBP] Cr (XbaI and PstI) Digestion of RBS[B0034] Ar (XbaI and PstI) backbone[pSB1A3] Ar (EcoRI and PstI) Terminator[J61048] Ar (XbaI and PstI) Electrophoresis of digested product: RBS[B0034] Ar (XbaI and PstI) backbone[pSB1A3] Ar (EcoRI and PstI) Terminator[J61048] Ar (XbaI and PstI) Ligation of OmpA[J23101+B0034+OmpA+pSB1A3] Ar GBP[GBP+B0034+pSB1K3] Kr OmpA[OmpA+J61048+pSB1K3] Kr
26

Ligation of RFP[E1010+B0034+pSB1K3] Kr Transformation of ligated product: OmpA[J23101+B0034+OmpA+pSB1A3] Ar GBP[FadL-GBP+B0034+pSB1K3] Kr OmpA[OmpA+J61048+pSB1K3] Kr Electrophoresis of digested product: RFP[E1010+J61048+pSB1K3] Kr [J23101+B0034] 		27

Digestion of Terminator[J61048] Ar (XbaI and PstI) PCR of ligated product OmpA[J23101+B0034+OmpA+pSB1A3] Ar OmpA[OmpA+J61048+pSB1K3] Kr 		28

Electrophoresis of RFP[E1010+J61048+pSB1K3] Kr OmpA[J23101+B0034+OmpA+pSB1A3] Ar Transformation of ligated product: GBP[B0034+FadL-GBP+pSB1A3] Ar OmpA[OmpA+J61048+pSB1K3] Kr PCR of ligated product OmpA[J23101+B0034+OmpA] Ar 		29

Cultivation of RFP[E1010+J61048+pSB1K3] Kr PCR of OmpA[J23101+B0034+OmpA] Ar Electrophoresis of OmpA[J23101+B0034+OmpA] Ar Ligation of GBP[FadL-GBP+J61048+pSB1K3] Kr 		30

Plasmid purification of RFP[E1010+J61048+pSB1K3] Kr Ligation of OmpA[J23101+B0034+OmpA] Ar PCR of OmpA[J23101+B0034+OmpA] Ar Transformation of mini: Terminator[J61048] ligated product: OmpA[J23101+B0034+OmpA] Ar
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

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3

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10

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17

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24

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31
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

 2

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7

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 10

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14

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 18

 19

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21

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28

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 30
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

PCR
transform 		2

 3

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5

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 11
12

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 18
19

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Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

PCR
transform
2

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8

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 15
16

 17

 18

 19

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23

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30

 31
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

PCR
transform 		2

 3

 4

 5
6

 7

 8

 9

 10

 11

 12
13

 14

 15

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 17

 18

 19
20

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