Difference between revisions of "Team:UNITN-Trento/Experiments"

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<h2><strong>TITLE OF THE SECTION</strong></h2>
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<h2><strong>Protocols for Characterization</strong></h2>  
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<li><p id="interlab_intro_btn" class="button small bstyle1" style="line-height:2em;" onclick="javascript:scrollToID('protocols_pr')"><span class="primary">Proteorhodopsin</span></p></li> 
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<li><p id="interlab_final_btn" class="button small bstyle7" style="line-height:2em;" onclick="javascript:scrollToID('protocols_pmfc')"><span class="primary">pMFC</span></p></li>
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<a class="anchor-off" name="protocols_pr" id="protocols_pr"></a>
Work in progress!
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<h3 class="wow fadeInDown">Proteorhodopsin</h3>
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Work in progress!
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<i class="liticon wow bounceInLeft flaticon-science3"></i>  <h4 class="header4 displayControl">Expression and Purification</h4>
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<i class="liticon wow bounceInLeft flaticon-dna9"></i> <h4 class="header4 displayControl">Anaerobic growth</h4>
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<i class="liticon wow bounceInLeft flaticon-dna9"></i> <h4 class="header4 displayControl">Proton-pumping measurements</h4>
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<i class="liticon wow bounceInLeft flaticon-dna9"></i> <h4 class="header4 displayControl">Solar mimicking apparatus</h4>
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<i class="liticon wow bounceInLeft flaticon-dna9"></i> <h4 class="header4 displayControl">Toxicity test by serial dilutions</h4>
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<div style="display:none;">
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<i class="liticon wow bounceInLeft flaticon-dna9"></i> <h4 class="header4 displayControl">ATP assay</h4>
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<div class="content">
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<section>
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<header>
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<h3 class="wow fadeInDown">15-15' Beta-carotene dioxygenase (BLH)</h3>
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</header>
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<i class="liticon wow bounceInLeft flaticon-science3"></i><h4 class="header4 displayControl">Cell growth</h4>
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<i class="liticon wow bounceInLeft flaticon-science3"></i><h4 class="header4 displayControl">Extraction of pigments</h4>
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<i class="liticon wow bounceInLeft flaticon-science3"></i><h4 class="header4 displayControl">TLC</h4>
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<i class="liticon wow bounceInLeft flaticon-science3"></i><h4 class="header4 displayControl">UV-VIS</h4>
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<i class="liticon wow bounceInLeft flaticon-science3"></i><h4 class="header4 displayControl">HPLC</h4>
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<div style="display:none;"></div>
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<div class="content">
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<section>
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<header>
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<h3 class="wow fadeInDown">Nicotinic Phosphoribosyl Transferase (pncB)</h3>
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</header>
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<i class="liticon wow bounceInLeft flaticon-science3"></i><h4 class="header4 displayControl">NAD/NADH quantification</h4>
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<h3 class="wow fadeInDown">pMFC</h3>
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<i class="liticon wow bounceInLeft flaticon-science3"></i><h4 class="header4 displayControl">Berkley bacteria growth</h4>
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<div style="display:none;"></div>
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<i class="liticon wow bounceInLeft flaticon-science3"></i><h4 class="header4 displayControl">pMFC data logging</h4>
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<div style="display:none;"></div>
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</div>
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</section> 
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Revision as of 16:44, 11 September 2015

Protocols for Characterization

  • Proteorhodopsin

  • BLH

  • PNCB

  • pMFC

Proteorhodopsin

Expression and Purification

Anaerobic growth

Proton-pumping measurements

Solar mimicking apparatus

Toxicity test by serial dilutions

ATP assay

15-15' Beta-carotene dioxygenase (BLH)

Cell growth

Extraction of pigments

TLC

UV-VIS

HPLC

Nicotinic Phosphoribosyl Transferase (pncB)

NAD/NADH quantification

pMFC

Berkley bacteria growth

pMFC data logging