Difference between revisions of "Team:Nankai/Practices"

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<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Nankai/miscCSS?action=raw&ctype=text/css" />
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#globalWrapper,section{
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<head>
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<title>Timeline - Free Responsive Template</title>
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        <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Team:Nankai/Practices/bootstrap.min?action=raw&ctype=text/css">
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         <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Team:Nankai/Practices/font-awesome.min?action=raw&ctype=text/css">
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         <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Team:Nankai/Practices/templatemo-style?action=raw&ctype=text/css">
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</style>
 
  
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        <!-- JavaScripts -->
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        <script src="https://2015.igem.org/Team:Nankai/Practices/jquery-1.10.2.min?action=raw&ctype=text/javascript"></script>
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</head>
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<body>
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    <div id="big-banner">
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        <div class="container">
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            <div class="row">
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                <div class="col-md-12 col-xs-12">
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                    <ul class="main-icons">
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                        <li><a href="#"></i><img src="images/iGEM Logo.png" width="127" height="97" alt=""/>iGEM</a></li>
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                        <li><a href="#"></i><img src="images/Nankai Univ.png" width="128" height="97" alt=""/>Nankai Univ.</a></li>
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                        <li><a href="#"></i><img src="images/iGEM Nankai Logo.png" width="97" height="97" alt=""/>iGEM_Nankai</a></li>
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                        <li><a href="#"></i><img src="images/iShare1.png" width="128" height="83" alt=""/>iShare</a></li>
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                    </ul>
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                </div>
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            </div>
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        </div>             
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    </div>
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    <div id="first-section">
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        <div class="heading">
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            <div class="container">
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                    <div class="col-md-12">
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                            <h2 class="header-section-title">What Did we do on Human practice</h2>
 +
                            <h4 id="1">Human Practices is the study of how your work affects the world, and how the world affects your work.</h4>
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 +
                  </div>
 +
                </div>
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            </div>
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        </div>
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    </div>
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    <div id="first-section1">
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        <div class="container">
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            <div class="row">
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                <div class="triangle"></div>
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            </div>
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            <div class="row">
 +
                <div class="col-md-12">
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                    <section id="cd-timeline" class="cd-container">
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                        <div class="cd-timeline-block">
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                            <div class="cd-timeline-img cd-picture">
 +
                                <img src="images/first-icon.png" alt="Picture">
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                            </div>
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 +
                            <div class="cd-timeline-content service-box-content">
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                                <h2>Communication</h2>
 +
                                <p>Communication  means improvement and achievement of Double-win. For example, Tianjin University  is not only our neighbor but also our good collaborator on experiment and human  practice.</p>
 +
                            </div>
 +
                        </div>
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 +
                        <div class="cd-timeline-block">
 +
                            <div class="cd-timeline-img cd-movie">
 +
                                <img src="images/second-icon.png" alt="Picture">
 +
                            </div>
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                            <div class="cd-timeline-content service-box-content">
 +
                                <h2>Cooperation</h2>
 +
                                <p>You can easily <strong>change top icons</strong> by looking at guidelines from <a rel="nofollow" href="http://fontawesome.info/font-awesome-icon-world-map/">Font Awesome</a>. Example: <strong>&lt;i class=&quot;fa fa-camera&quot;&gt;&lt;/i&gt;</strong></p>
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                            </div>
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                        </div>
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                        <div class="cd-timeline-block">
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                            <div class="cd-timeline-img cd-icon">
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                                <img src="images/third-icon.png" alt="Picture">
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                            </div>
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                            <div class="cd-timeline-content service-box-content">
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                                <h2>Conferences</h2>
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                                <p>Not  only can we get precious ideas from experts and other teams in conference but also broaden  our horizons on synthetic biology</p>
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</div>
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                        </div>
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                        <div class="cd-timeline-block">
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                            <div class="cd-timeline-img cd-location">
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                                <img src="images/fourth-icon.png" alt="Picture">
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                            </div>
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                            <div class="cd-timeline-content service-box-content">
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                                <h2>iShare</h2>
 +
                                <p>iShare as  we pictured is the first step to an  all-round resource sharing platform. </p>
 +
                            </div>
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                        </div>
 +
                    </section>
 +
                </div>
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            </div>
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        </div>
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    </div>
 +
    <div class="copyrights">Collect from <a href="http://www.cssmoban.com/"  title="网站模板">网站模板</a></div>
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 +
    <div id="cta">
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        <div class="container">
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            <div class="row">
 +
                <div class="col-md-12">
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                    <h4>Communications</h4>
 +
                    <p>What we have done</p>
 +
                </div> 
 +
            </div>
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        </div>
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    </div>
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 +
    <div id="second-section">
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        <div class="heading">
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            <div class="container">
 +
                <div class="row">
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                    <div class="col-md-12">
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                            <h2>Latest Work wiht TJU iGEM team</h2>
 +
                            <h4>Communication and Win-win cooperation</h4>
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 +
                    </div>
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                </div>
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            </div>
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        </div>
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    </div>
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    <div id="second-section2">
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        <div class="container">
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            <div class="row">
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                <div class="triangle"></div>
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            </div>
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            <div class="row">
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                <div class="col-md-12">
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                    <section id="cd-timeline-2" class="cd-container">
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                        <div class="cd-timeline-block">
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                            <div class="cd-timeline-img cd-picture">
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                                <img src="images/camera-icon.png" alt="Picture">
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                            </div>
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                            <div class="cd-timeline-content projects">
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                                <img src="https://static.igem.org/mediawiki/2015/1/1f/Nankai_HP_project1.jpg" alt="">
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                          </div>
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                        </div>
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                        <div class="cd-timeline-block">
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                            <div class="cd-timeline-img cd-picture">
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                                <img src="images/camera-icon.png" alt="Picture">
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                            </div>
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 +
                            <div class="cd-timeline-content projects">
 +
                                <img src="https://static.igem.org/mediawiki/2015/b/b1/Nankai_HP_project2.jpg" alt="">
 +
                               
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                          </div>
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                        </div>
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                        <div class="cd-timeline-block">
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                            <div class="cd-timeline-img cd-picture">
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                                <img src="images/camera-icon.png" alt="Picture">
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                            </div>
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 +
                            <div class="cd-timeline-content projects">
 +
                                <img src="https://static.igem.org/mediawiki/2015/0/0b/Nankai_HP_project3.jpg" alt="">
 +
                               
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                          </div>
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                        </div>
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                        <div class="cd-timeline-block">
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                            <div class="cd-timeline-img cd-picture">
 +
                                <img src="images/camera-icon.png" alt="Picture">
 +
                            </div>
 +
 +
                            <div class="cd-timeline-content projects">
 +
                                <img src="https://static.igem.org/mediawiki/2015/d/d7/Nankai_HP_project4.jpg" alt="">
 +
                               
 +
                          </div>
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                        </div>
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                    </section>
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                </div>
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            </div>
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        </div>
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    </div>
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<div id="second-section">
 +
        <div class="heading">
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            <div class="container">
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                <div class="row">
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                  <div class="col-md-12">
 +
                    <h2>MEETING With  ZJU &amp; SYSU iGEM team</h2>
 +
                          <h4>Until now, we have built up the  collaboration with TJU, ZJU and SYSU. In the near future, we will further  communicate with more universities and build up collaborations. We have the  same wish for a better team! Let&rsquo;s communicate and collaborate! </h4>
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                    </div>
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                </div>
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            </div>
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        </div>
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    </div>
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    <div id="second-section2">
 +
        <div class="container">
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            <div class="row">
 +
                <div class="triangle"></div>
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            </div>
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            <div class="row">
 +
                <div class="col-md-12">
 +
                    <section id="cd-timeline-2" class="cd-container">
 +
                        <div class="cd-timeline-block">
 +
                            <div class="cd-timeline-img cd-picture">
 +
                                <img src="images/camera-icon.png" alt="Picture">
 +
                            </div>
 +
 +
                            <div class="cd-timeline-content projects">
 +
                                <img src="https://static.igem.org/mediawiki/2015/2/23/Nankai_HP_project6.jpg" alt="">
 +
                               
 +
                          </div>
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                        </div>
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                        <div class="cd-timeline-block">
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                            <div class="cd-timeline-img cd-picture">
 +
                                <img src="images/camera-icon.png" alt="Picture">
 +
                            </div>
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 +
                            <div class="cd-timeline-content projects">
 +
                                <img src="https://static.igem.org/mediawiki/2015/1/10/Nankai_HP_project7.jpg" alt="">
 +
                               
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                          </div>
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                        </div>
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                        <div class="cd-timeline-block">
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                            <div class="cd-timeline-img cd-picture">
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                                <img src="images/camera-icon.png" alt="Picture">
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                            </div>
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 +
                            <div class="cd-timeline-content projects">
 +
                                <img src="https://static.igem.org/mediawiki/2015/8/87/Nankai_HP_project8.jpg" alt="">
 +
                               
 +
                          </div>
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                        </div>
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 +
                        <div class="cd-timeline-block">
 +
                            <div class="cd-timeline-img cd-picture">
 +
                                <img src="images/camera-icon.png" alt="Picture">
 +
                            </div>
 +
 +
                            <div class="cd-timeline-content projects">
 +
                                <img src="https://static.igem.org/mediawiki/2015/d/da/Nankai_HP_project9.jpg" alt="">
 +
                               
 +
                          </div>
 +
                        </div>
 +
                    </section>
 +
                </div>
 +
            </div>
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        </div>
 +
    </div>
 +
<div id="cta">
 +
        <div class="container">
 +
            <div class="row">
 +
              <div class="col-md-12">
 +
                <h4>Cooperations</h4>
 +
                  <p>Let's do experiments better!          </p>
 +
                </div>
 +
            </div>
 +
        </div>
 +
    </div>
 +
 
 +
    </div>
 +
    <div id="second-section">
 +
        <div class="heading">
 +
            <div class="container">
 +
                <div class="row">
 +
                  <div class="col-md-12">
 +
                    <div class="col-md-12">
 +
                      <h2>Experiment materials sharing with TJU</h2>
 +
                      <h4>
 +
                        <p>This year, the igem team of Tianjin Universitiy is working on a special hydrophobin HGFII. Apart from the gene of IGFII, they also own the gene of HGFI. However, what they did is the prokaryotic expression in escherichia coli, so they lacked the conditions in eucells to test this gene. Coincidengly, there is a professor in our school which is working on the fermentation of HGFI. Hearing this, we visited his lab and successfully obtained these statistics. And finally, We support these data for their team.</p>
 +
                        <p>In the below are the real HPLC（High Performance Liquid Chromatography) results. From these pictures we can see that in the pure products the peak appeared at around 30 minutes while at the fermentation broth the peak appeared at aound 33 minutes.</p>
 +
                      </h4>
 +
                    </div>
 +
                    <h4><P>&nbsp;</p></h4>
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                  </div>
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                </div>
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            </div>
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        </div>
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    </div>
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    <div id="second-section2">
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        <div class="container">
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            <div class="row">
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                <div class="triangle"></div>
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            </div>
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            <div class="row">
 +
                <div class="col-md-12">
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              <section id="cd-timeline-2" class="cd-container">
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                        <div class="cd-timeline-block">
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                            <div class="cd-timeline-img cd-picture">
 +
                                <img src="images/camera-icon.png" alt="Picture">
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                            </div>
 +
 +
                            <div class="cd-timeline-content projects">
 +
                              <img src="https://static.igem.org/mediawiki/2015/4/48/Nankai_HP_project10.jpg" alt="">
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                            </div>
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                        </div>
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                    <h4>Conferences</h4>
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                    <p>Wilder horizons</p>
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                      <h2>The 3rd SYMPOSIUM OF BOHAI-RIM MICROBIOLOGY
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</h2>
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                            <h4><p>Dalian City, Liaoning Province</p><p><img src="https://static.igem.org/mediawiki/2015/1/15/Nankai_HP_BOHAI_RIM.jpg" alt="" width="1035" height="479" align="absmiddle"/></p> </h4>
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                    <h2>iGEM MEETUP：Peking CCiC</h2>
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                            <h4><p>Peking Univeristy, Beijing</p>
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                            <p><img src="https://static.igem.org/mediawiki/2015/5/58/Nankai_HP_PEKING_CCIC.jpg" alt="" width="1007" height="501" align="absmiddle"/></P></h4>
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                    <h4>iShare</h4>
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                    <p>Resource Sharing Platform</p>
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                      <h2><img src="https://static.igem.org/mediawiki/2015/a/a2/Nankai_HP_iShare_Info.jpg" width="261" height="71" alt=""/>iShare Introduction</h2>
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                      <h4>As we all know, iGEM has already established a functional platform for all the participators to share bio-bricks. However, the information about materials related to those Bio-bricks are still missing. These things are also important information. Where can we get these information? So we come up with the idea: iShare.</h4>
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                    <p>Your place:&nbsp;<a href="https://2015.igem.org/Team:Nankai">Home</a>&nbsp;&gt;&nbsp;<a href="https://2015.igem.org/Team:Nankai/Description">Project</a></p>
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                      <h2><img src="https://static.igem.org/mediawiki/2015/8/8d/Nankai_HP_iShare_Survey.jpg" width="241" height="66" alt=""/>iShare Survey</h2>
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                      <h4>To make iShare betetr, we sent online survey invitaions to more than 280 iGEM teams </h4>
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<h4>What is γ-PGA?</h4>
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<p>Poly-γ-glutamic acid (γ-PGA) is an important, naturally occurring polyamide consisting of D/L-glutamate monomers. Unlike typical peptide linkages, the amide linkages in γ-PGA are formed between the α-amino group and the γ-carboxyl group. γ-PGA exhibits many favorable features such as biodegradable, water soluble, edible and non-toxic to humans and the environment. Therefore, it has been widely used in fields of foods, medicines, cosmetics and agriculture and many unique applications, such as a sustained release material and drug carrier, curable biological adhesive, biodegradable fibres, and highly water absorbable hydrogels.</p>
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<p>Strains capable for producing γ-PGA are divided into two categories based on their requirement for glutamate acid: glutamate-dependent strains and glutamate-independent strains. Glutamate-independent strains are preferable for industrial production because of their low cost and simplified fermentation process. However, compared with glutamate-dependent strains, their lower γ-PGA productivity limits their industrial application.Therefore, the construction of a glutamate-independent strain with high γ-PGA yield is important for industrial applications.</p>
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<p>Bacillusamyloliquefaciens LL3, isolated from fermented food, is a glutamate-independent strain, which can produce 3-4 g/L γ-PGA with sucrose as its carbon source and ammonium sulfate as its nitrogen source. The B. amyloliquefaciens LL3 strain was deposited in the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 208109 and its whole genome has been sequenced in 2011. In this study, we aimed to improve the γ-PGA production based on the B. amyloliquefaciens NK-1 strain (a derivative of LL3 strain with its endogenous plasmid and upp gene deleted).</p>
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<p>In order to improve γ-PGA production, we employed two strategies to fine-tune the synthetic pathways and balance the metabolism in the glutamate-independent B. amyloliquefaciens NK-1 strain. Firstly, we constructed a metabolic toggle switch in the NK-1 strain to inhibit the expression of ODHC (2-oxoglutarate dehydrogenase complex) by adding IPTG in the stationary stage and distribute the metabolic flux more frequently to be used for γ-PGA precursor-glutamate synthesis. As scientists had found that the activity of ODHC was rather low when glutamate was highly produced in a Corynebacterium glutamicum strain. Second, to balance the increase of endogenous glutamate production, we optimized the expression level of pgsBCA genes (responsible for γ-PGA synthesis) by replacing its native promoter to seven different strength of promoters. Through these two strategies, we aimed to obtain a γ-PGA production improved mutant strain.<a href="https://2015.igem.org/Team:Nankai/Experiments">Click for more detail.</a></p>
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<p>We prepared SOD loaded γ-PGA hydrogel for wound healing. SOD was loaded into hydrogels to scavenge the superoxide anion and γ-PGA was modified with taurine to load more SOD.  γ-PGA hydrogel had high water absorption properties delivering the important moist environment. SOD released from the hydrogel maintained high enzyme activity and SOD-γ-PGA hydrogel could scavenge the superoxide anion effectively. In vivo results showed that  SOD-γ-PGA hydrogel could promote collagen deposition, epithelialization, and accelerate the healing of moderately exuding wounds. Therefore, SOD-γ-PGA hydrogel would be a good candidate for wound healing applications.<a href="https://2015.igem.org/Team:Nankai/pudding_health_kit">Click for more detail.</a></p>
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<h6><a href="https://2015.igem.org/Team:Nankai/Description">Description</a></h6>
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                                                <p>Preparing for LB medium.</p>
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                                                <p>Cultured LL3.</p>
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                                                <p>In the progress of fermentation.</p>
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                      <h4> Now we have sent e-mails to more than 30 teams and more and more teams are joining together. What’s more, in the near future, we would like to transform iShare form a wiki to a PC software or Mobile phone App to make it more convenient for information sharing. </h4>
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<h6>References</h6>
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<p>1. Ashiuchi, M., Misono, H., 2002. Biochemistry and molecular genetics of poly-γ-glutamate synthesis. Appl. Biochem. Biotechnol. 59, 9–14.</br>
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2. Kunioka, M., 1997. Biosynthesis and chemical reactions of poly(amino acid)s from
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microorganisms. Appl. Microbiol. Biotechnol. 47, 469–475.</br>
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3. Shih, I.L., Van, Y.T., 2001. The production of poly(γ-glutamic acid) from microorganism and its various applications. Bioresour. Technol. 79, 207–225.</br>
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4. Li, C., 2002. Poly(L-glutamic acid)--anticancer drug conjugates. Adv. Drug Deliver. Rev. 54, 695–713.</br>
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5. Liang, H.F., Chen, C.T., Chen, S.C., Kulkarni, A.R., Chiu, Y.L., Chen, M.C., Sung, H.W., 2006. Paclitaxel-loaded poly(γ-glutamic acid)-poly(lactide) nanoparticles as a targeted drug delivery system for the treatment of liver cancer. Biomaterials. 27, 2051–2059.</br>
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6. Richard, A., Margaritis, A., 2001. Poly (glutamic acid) for biomedical applications. Crit. Rev. Biotechnol. 21, 219–232.</br>
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7. Park, Y.J., Liang, J., Yang, Z., Yang, V.C., 2001. Controlled release of clot-dissolving tissue-type plasmmogen activator from a poly(L-glutamic acid) semi-interpenetrating polymer network hydrogel. J. Control. Release. 74, 243–247.</br>
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8. Cao, M.F., Geng, W.T., Liu, L., Song, C.J., Xie, H., Guo, W.B., Jin, Y.H., Wang, S.F., 2011. Glutamic acid independent production of poly-γ-glutamic acid by Bacillus amyloliquefaciens LL3 and cloning of pgsBCA genes. Bioresour. Technol. 102, 4251–4257.</br>
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9. Geng, W.T., Cao, M.F., Song, C.J., Xie, H., Liu, L., Yang, C., Feng, J., Zhang, W., Jin, Y.H., Du, Y., Wang, S.F., 2011. Complete genome sequence of Bacillus amyloliquefaciens LL3, which exhibits glutamic acid-independent production of poly-γ-glutamic acid. J. Bacteriol. 193, 3393–3394.</br>
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10. Feng, J., Gao, W.X., Gu, Y.Y., Zhang, W., Cao, M.F., Song, C.J., Zhang, P., Sun, M., Yang, C.,  Wang, S.F., 2014a. Functions of poly-gamma-glutamic acid (γ-PGA) degradation genes in γ-PGA synthesis and cell morphology maintenance. Appl. Microbiol. Biotechnol. 98, 6397–6407.</br>
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11. Uy, D., Delaunay S., Germain, P., Engasser, J.M., Goergen, J.L. 2003. Instability of glutamate production by Corynebacterium glutamicum 2262 in continuous culture using the temperature-triggered process. J. Biotech. 104, 173-184.</p>
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Revision as of 16:27, 12 September 2015

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What Did we do on Human practice

                            
Human Practices is the study of how your work affects the world, and how the world affects your work.


                  
 
                

            

        

    


    

        

            

                

            

            

                

                    

                        

                            

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Communication

                                
Communication  means improvement and achievement of Double-win. For example, Tianjin University  is not only our neighbor but also our good collaborator on experiment and human  practice.

                            

                        


                        

                            

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Cooperation

                                
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Conferences

                                
Not  only can we get precious ideas from experts and other teams in conference but also broaden  our horizons on synthetic biology



                        
 

                        

                            

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iShare

                                
iShare as  we pictured is the first step to an  all-round resource sharing platform. 

                            

                        

                    

                

            

        

    

    
Collect from 网站模板


    

        

            

                

                    
Communications

                    
What we have done

                
   
            

        

    


    

        

            

                

                    

                            
Latest Work wiht TJU iGEM team

                            
Communication and Win-win cooperation


                    
 
                

            

        

    

    
    

        

            

                

            

            

                

                    

                        

                            

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MEETING With  ZJU & SYSU iGEM team

                          
Until now, we have built up the  collaboration with TJU, ZJU and SYSU. In the near future, we will further  communicate with more universities and build up collaborations. We have the  same wish for a better team! Let’s communicate and collaborate! 


                    
 
                

            

        

    

    
    

        

            

                

            

            

                

                    

                        

                            

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Cooperations

                  
Let's do experiments better!

Experiment materials sharing with TJU

This year, the igem team of Tianjin Universitiy is working on a special hydrophobin HGFII. Apart from the gene of IGFII, they also own the gene of HGFI. However, what they did is the prokaryotic expression in escherichia coli, so they lacked the conditions in eucells to test this gene. Coincidengly, there is a professor in our school which is working on the fermentation of HGFI. Hearing this, we visited his lab and successfully obtained these statistics. And finally, We support these data for their team.

In the below are the real HPLC（High Performance Liquid Chromatography) results. From these pictures we can see that in the pure products the peak appeared at around 30 minutes while at the fermentation broth the peak appeared at aound 33 minutes.

 

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Conferences

Wilder horizons

The 3rd SYMPOSIUM OF BOHAI-RIM MICROBIOLOGY

Dalian City, Liaoning Province

iGEM MEETUP：Peking CCiC

Peking Univeristy, Beijing

iShare

Resource Sharing Platform

iShare Introduction

As we all know, iGEM has already established a functional platform for all the participators to share bio-bricks. However, the information about materials related to those Bio-bricks are still missing. These things are also important information. Where can we get these information? So we come up with the idea: iShare.

iShare Survey

To make iShare betetr, we sent online survey invitaions to more than 280 iGEM teams

iShare Now and Futrue

Now we have sent e-mails to more than 30 teams and more and more teams are joining together. What’s more, in the near future, we would like to transform iShare form a wiki to a PC software or Mobile phone App to make it more convenient for information sharing.