Difference between revisions of "Team:Aalto-Helsinki/InterLab"
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<h3>Cultivations and measurement</h3> | <h3>Cultivations and measurement</h3> | ||
| − | <p>Followed the next protocol for preparing the samples:</br></br>Streaked out LB-plates with E.coli TOP10 strain containing every device and control with chloramphenicol concentration of 35 μg/ml. Incubated plates overnight (18-20 hours) at 37\(^\circ\)C. Picked up biological triplates from the plates and inoculated 3 ml liquid cultures from the colonies with 12 ml polypropylene test tube. Incubated the tubes at 37\(^\circ\)C with shaking at 300rpm for 18 hours. Measured OD600 values of cultures in cuvettes and calculated the dilution required for each sample to obtain the OD value of 0.5. Diluted and re-measured the samples to reach 5% accuracy of 0.5. Transfered 200 μl of each sample into 96-well transparent plate and set the platereader to read fluorescence intensity. Proceed with the measurements with the following equipment: </br> </br>Type: Cell Imaging Multi-Mode Plate Reader </br> | + | <p>Followed the next protocol for preparing the samples:</br></br>Streaked out LB-plates with E.coli TOP10 strain containing every device and control with chloramphenicol concentration of 35 μg/ml. Incubated plates overnight (18-20 hours) at 37\(^\circ\)C. Picked up biological triplates from the plates and inoculated 3 ml liquid cultures from the colonies with 12 ml polypropylene test tube. Incubated the tubes at 37\(^\circ\)C with shaking at 300rpm for 18 hours. Measured OD600 values of cultures in cuvettes and calculated the dilution required for each sample to obtain the OD value of 0.5. Diluted and re-measured the samples to reach 5% accuracy of 0.5. Transfered 200 μl of each sample into 96-well transparent plate and set the platereader to read fluorescence intensity. Proceed with the measurements with the following equipment: </br> </br><b>Type:</b> Cell Imaging Multi-Mode Plate Reader </br> |
| − | Name and model: Cytation 3 </br> | + | <b>Name and model:</b> Cytation 3 </br> |
| − | Company: BioTek Instruments, Inc</br></br> | + | <b>Company:</b> BioTek Instruments, Inc</br></br> |
The following parametres were used:</br></br> | The following parametres were used:</br></br> | ||
| − | Excitation wavelength: 470 nm</br>Emission wavelength: 511 nm</br>Optics Position: Bottom </br> Filter: Quadruple Monochromators with Bandwith of 16 nm</br> Units Reported: RFU</br></br></p> | + | <b>Excitation wavelength:</b> 470 nm</br><b>Emission wavelength:</b> 511 nm</br><b>Optics Position:</b> Bottom </br> <b>Filter:</b> Quadruple Monochromators with Bandwith of 16 nm</br> <b>Units Reported:</b> RFU</br></br></p> |
</section> | </section> | ||
Revision as of 21:28, 12 September 2015
