Difference between revisions of "Team:Gifu/Experiment-page"
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<a name="descri" style="text-decoration:none;"></a> <br> | <a name="descri" style="text-decoration:none;"></a> <br> | ||
<hr width="100%" > <h4> <font size="5" face="Century"> EXPERIMENTS </font> </h4> | <hr width="100%" > <h4> <font size="5" face="Century"> EXPERIMENTS </font> </h4> | ||
− | <h6><font size="4" face="Century"> Making functional long chain protein </font><br></h6> | + | <h6><font size="4" face="Century" > Making functional long chain protein </font><br></h6> |
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<h5><p> In our last study, our long protein lost its function. As the reason, we have the idea that the protein lost its holding because it was made be long. For the reason of this problem, we have the idea that being long protein chain destructs its holding. So in this year, we aim to synthesize functional long chain proteins by designing them including some linkers. <br> | <h5><p> In our last study, our long protein lost its function. As the reason, we have the idea that the protein lost its holding because it was made be long. For the reason of this problem, we have the idea that being long protein chain destructs its holding. So in this year, we aim to synthesize functional long chain proteins by designing them including some linkers. <br> | ||
The requirements for amino acids that con be the linkers are less of steric effects and strong in hydropathy. Glycine and serine fulfill this requirements. Glycine’ side chain has almost no steric effects. On the other hand, serine has some steric effects but it has powerful hydropathy because of its hydroxyl side chain. According to やべっち, because histidine has quite strong hydropathy, though it has big steric effects, it is possible that histidine can work as linker.<br> | The requirements for amino acids that con be the linkers are less of steric effects and strong in hydropathy. Glycine and serine fulfill this requirements. Glycine’ side chain has almost no steric effects. On the other hand, serine has some steric effects but it has powerful hydropathy because of its hydroxyl side chain. According to やべっち, because histidine has quite strong hydropathy, though it has big steric effects, it is possible that histidine can work as linker.<br> | ||
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<h6><font size="4" face="Century"> developing the efficiency of circularization </font><br></h6> | <h6><font size="4" face="Century"> developing the efficiency of circularization </font><br></h6> | ||
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<h5><p> Figure: prediction of secondary structure of td gene intron and around splicing site<br> | <h5><p> Figure: prediction of secondary structure of td gene intron and around splicing site<br> | ||
Because the complementary sequence designed inside the splicing site is included circular mRNA, its sequence is translated into amino acids. So we designed this sequence as complementarity and efficiency.<br> | Because the complementary sequence designed inside the splicing site is included circular mRNA, its sequence is translated into amino acids. So we designed this sequence as complementarity and efficiency.<br> |
Revision as of 01:28, 13 September 2015