Difference between revisions of "Team:Queens Canada/Safety"

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<h2>Safety in iGEM</h2>
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h4>Safe Project Design</h4>
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            <li><a href="https://2015.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2015/f/fa/Qqq_IGEM_official_logo.png" /></a></li>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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            <li><a href="https://2015.igem.org/Team:Queens_Canada/Sponsorship">Sponsorship</a></li>
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            <li><a href="https://2015.igem.org/Team:Queens_Canada/Attributions">Attributions</a></li>
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            <li><a href="https://2015.igem.org/Team:Queens_Canada/Safety">Safety</a></li>
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<li>Choosing a non-pathogenic chassis</li>
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            <li><a href="https://2015.igem.org/Team:Queens_Canada/Practices">Human Practices</a>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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                <ul>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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                    <li><a href="https://2015.igem.org/Team:Queens_Canada/Practices/Academics">Academics</a></li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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                    <li><a href="https://2015.igem.org/Team:Queens_Canada/Practices/Outreach">Community Outreach</a></li>
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                    <li><a href="https://2015.igem.org/Team:Queens_Canada/Practices/IP">Intellectual Property</a></li>
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<h4>Safe Lab Work</h4>
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                </ul>
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            <li><a href="https://2015.igem.org/Team:Queens_Canada/Notebook">Notebook</a></li>
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            <li><a href="https://2015.igem.org/Team:Queens_Canada/Parts">Parts</a></li>
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            <li><a href="https://2015.igem.org/Team:Queens_Canada/Description">Project</a>
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                <ul>
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                    <li><a href="https://2015.igem.org/Team:Queens_Canada/Background">Background</a></li>
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                    <li><a href="https://2015.igem.org/Team:Queens_Canada/Modeling">Modeling</a></li>
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                    <li><a href="https://2015.igem.org/Team:Queens_Canada/AFP_Scaffold"> AFP-Scaffold Complex</a></li>
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                    <li><a href="https://2015.igem.org/Team:Queens_Canada/Circ_AFP">Circularized AFP</a></li>
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                </ul>
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            <li><a href="https://2015.igem.org/Team:Queens_Canada/Team">Team</a></li>
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            <li><a href="https://2015.igem.org/Team:Queens_Canada">Home</a></li>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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    <h1>Safety at QGEM 2015</h1>
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    <p>All QGEM members successfully completed extensive lab safety training, including Workplace Hazardous Materials Information System (WHMIS) and BioSafety Training Level 2 to ensure complete safety. All work was performed in research labs with lab technicians and graduate students present during working hours. WHMIS clarified our understanding of any potentially unsafe material we may encounter in the lab such as ethidium bromide, highly concentrated nitric acid, etc. Ethidium bromide was used to visualize DNA on agarose gels, and highly concentrated nitric acid was used for cleaning of certain lab equipment. Any lab members helping to clean this equipment ensured that they wore personal protective equipment (PPE; gloves, lab coats, even face masks). Extra care was taken to ensure that all hazardous materials were used safely and disposed properly.</p>
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    <p>Low individual and community risk (Risk Group 1) cloning strains of E. coli (K12 strains) in regulation with Canadian Biosafety Standards were used for expressing our constructs. These are non-pathogenic strains of E. coli and do not pose any threat to laboratory workers, the general public, or the environment. Biosafety regulations were followed at all times to ensure that any modified E.coli strains were not introduced to the environment or other people. </p>
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<h4>Safe Shipment</h4>
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    <div class="footer">
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        <a href="https://www.facebook.com/iGEMQueens?fref=ts"><img src="https://static.igem.org/mediawiki/2015/2/29/Qqq_QGEM_FB_FindUsOnFacebook.png" /> </a>
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        <a href="https://twitter.com/igemqueens"><img id="twitter" src="https://static.igem.org/mediawiki/2015/3/34/Qqq_QGEM_twitterlogo.png" /></a>
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Revision as of 17:30, 13 September 2015

Safety at QGEM 2015

All QGEM members successfully completed extensive lab safety training, including Workplace Hazardous Materials Information System (WHMIS) and BioSafety Training Level 2 to ensure complete safety. All work was performed in research labs with lab technicians and graduate students present during working hours. WHMIS clarified our understanding of any potentially unsafe material we may encounter in the lab such as ethidium bromide, highly concentrated nitric acid, etc. Ethidium bromide was used to visualize DNA on agarose gels, and highly concentrated nitric acid was used for cleaning of certain lab equipment. Any lab members helping to clean this equipment ensured that they wore personal protective equipment (PPE; gloves, lab coats, even face masks). Extra care was taken to ensure that all hazardous materials were used safely and disposed properly.

Low individual and community risk (Risk Group 1) cloning strains of E. coli (K12 strains) in regulation with Canadian Biosafety Standards were used for expressing our constructs. These are non-pathogenic strains of E. coli and do not pose any threat to laboratory workers, the general public, or the environment. Biosafety regulations were followed at all times to ensure that any modified E.coli strains were not introduced to the environment or other people.