Difference between revisions of "Team:Northeastern Boston/BasicParts"
| Line 86: | Line 86: | ||
<p>Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from <a href="http://chlamycollection.org/plasmid/popt_mvenus_hyg" target="_blank">pOpt_mVenus_Hyg</a> and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any <i>Chlamydomonas reinhardtii</i> transformed with this part.</p> | <p>Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from <a href="http://chlamycollection.org/plasmid/popt_mvenus_hyg" target="_blank">pOpt_mVenus_Hyg</a> and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any <i>Chlamydomonas reinhardtii</i> transformed with this part.</p> | ||
| − | <p>This part contains intron 2 of RBCS2 after a fusion HSP70A-RBCS2 promoter. [<a href="http://onlinelibrary.wiley.com/store/10.1046/j.1365-313x.2000.00652.x/asset/j.1365-313x.2000.00652.x.pdf?v=1&t=iejf1jm1&s=f3a9125d087876eeeb8fe8892916ac1a9a96c3a9" target="_blank">1</a>] Aph7'', a phosphotransferase, confers resistance to Hygromycin B at agar antibiotic concentrations up to 200g/mL.[<a href="http://www.sciencedirect.com/science/article/pii/S1434461004701170" target="_blank">2</a>]</p> | + | <p>This part contains intron 2 of RBCS2 after a fusion HSP70A-RBCS2 promoter. [<a href="http://onlinelibrary.wiley.com/store/10.1046/j.1365-313x.2000.00652.x/asset/j.1365-313x.2000.00652.x.pdf?v=1&t=iejf1jm1&s=f3a9125d087876eeeb8fe8892916ac1a9a96c3a9" target="_blank">1</a>] Aph7'', a phosphotransferase, confers resistance to Hygromycin B at agar antibiotic concentrations up to 200g/mL. [<a href="http://www.sciencedirect.com/science/article/pii/S1434461004701170" target="_blank">2</a>]</p> |
<h2>mVenus Fluorescent Protein</h2> | <h2>mVenus Fluorescent Protein</h2> | ||
Revision as of 05:18, 14 September 2015
BASIC & COMPOSITE PARTS
Hygromycin B Resistance Cassette
Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from pOpt_mVenus_Hyg and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transformed with this part.
This part contains intron 2 of RBCS2 after a fusion HSP70A-RBCS2 promoter. [1] Aph7'', a phosphotransferase, confers resistance to Hygromycin B at agar antibiotic concentrations up to 200g/mL. [2]
mVenus Fluorescent Protein
This part contains mVenus, a nuclear codon-optimized YFP variant (excitation/emission: 515/528nm) [3]. It has Intron 1 and 2 of RBCS2 for increased expression. This is the first independent fluorescent protein codon-optimized for Chlamydomonas reinhardtii submitted to the iGEM registry.