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Revision as of 05:28, 14 September 2015

Lab note
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Sun. Mon. Tue. Wed. Thu. Fri. Set.
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Transformation of
GBP[FadL-GBP]
Cultivation of
OmpA[OmpA+pSB1K3] Kr 		16

Cultivation of
GBP[FadL-GBP]
Plasmid purification of
OmpA[OmpA+pSB1K3] Kr
Digestion of
OmpA[OmpA+pSB1K3] Kr (EcoRI and PstI) 		17

Plasmid purification of
GBP[FadL-GBP]
Digestion of
GBP[FadL-GBP] (EcoRI and PstI)
Electrophoresis of digested product:
GBP[FadL-GBP] (EcoRI and PstI) 		18

Transformation of ligated product:
GBP[FadL-GBP+pSB1C3] Cr
19

Cultivation of
ligated product: GBP[FadL-GBP] Cr
PCR product: Terminator[J61048] 		20

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Cultivation of
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar
OmpA[OmpA+pSB1C3] Cr
GBP[FadL-GBP+pSB1C3] Cr 		22

Plasmid purification of
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar
OmpA[OmpA+pSB1C3] Cr
GBP[FadL-GBP+PSB1C3] Cr
Digestion of
GFP[J23101+B0034+E0040+J61048] Ar (EcoRI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
GBP[FadL-GBP] Cr (EcoRI and PstI)
Electrophoresis of digested product:
GFP[J23101+B0034+E0040+J61048] Ar (EcoRI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
GBP[FadL-GBP] Cr (EcoRI and PstI) 		23

Digestion of OmpA[OmpA] Cr (EcoRI and SpeI)
GBP[FadL-GBP] Cr (EcoRI and SpeI)
Electrophoresis of digested product:
OmpA[OmpA] Cr (EcoRI and SpeI)
GBP[FadL-GBP] Cr (EcoRI and SpeI) 		24

Digestion of
OmpA[OmpA] Cr (XbaI and PstI)
GBP[FadL-GBP] Cr (XbaI and PstI) 		25

Electrophoresis of digested product:
OmpA[OmpA] Cr (XbaI and PstI)
GBP[FadL-GBP] Cr (XbaI and PstI)
Digestion of
RBS[B0034] Ar (XbaI and PstI)
backbone[pSB1A3] Ar (EcoRI and PstI)
Terminator[J61048] Ar (XbaI and PstI)
Electrophoresis of digested product:
RBS[B0034] Ar (XbaI and PstI)
backbone[pSB1A3] Ar (EcoRI and PstI)
Terminator[J61048] Ar (XbaI and PstI)
Ligation of
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
GBP[GBP+B0034+pSB1K3] Kr
OmpA[OmpA+J61048+pSB1K3] Kr
26

Ligation of
RFP[E1010+B0034+pSB1K3] Kr
Transformation of ligated product:
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
GBP[FadL-GBP+B0034+pSB1K3] Kr
OmpA[OmpA+J61048+pSB1K3] Kr
Electrophoresis of digested product:
RFP[E1010+J61048+pSB1K3] Kr
[J23101+B0034] 		27

Digestion of
Terminator[J61048] Ar (XbaI and PstI)
PCR of ligated product:
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
OmpA[OmpA+J61048+pSB1K3] Kr 		28

Electrophoresis of
RFP[E1010+J61048+pSB1K3] Kr
OmpA[J23101+B0034+OmpA+pSB1A3] Ar Transformation of ligated product:
GBP[B0034+FadL-GBP+pSB1A3] Ar
OmpA[OmpA+J61048+pSB1K3] Kr PCR of ligated product
OmpA[J23101+B0034+OmpA] Ar 		29

Cultivation of
RFP[E1010+J61048+pSB1K3] Kr PCR of
OmpA[J23101+B0034+OmpA] Ar
Electrophoresis of
OmpA[J23101+B0034+OmpA] Ar Ligation of
GBP[FadL-GBP+J61048+pSB1K3] Kr 		30

Plasmid purification of
RFP[E1010+J61048+pSB1K3] Kr Ligation of
OmpA[J23101+B0034+OmpA] Ar PCR of
OmpA[J23101+B0034+OmpA] Ar Transformation of
mini: Terminator[J61048]
ligated product: OmpA[J23101+B0034+OmpA] Ar
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