Collaborations

Collaboration is an important part of the iGEM competition. We worked with three different 2015 iGEM teams to achieve different goals.

Manchester-Graz team

The Manchester-Graz team are also working on a project centred around the gut microbiome. We learned this because our PhD adviser, Sibyl, met their supervisor at a carbohydrate conference in May 2015.

Manchester-Graz have developed an expression system designed to regulate single and multi-gene pathways for an intestine expression. They help us to model butyrate production in the gut and we also exchanged parts to conduct reciprocal testing experiments measuring output by High Performance Liquid Chromatography (HPLC). Additionally we tested their parts by incubating them in our faecal microbiota system.

You can learn more by clicking on the image on the right.

Valencia (UPV) team

The Valencia (UPV) team are also working on plants (along with the Cambridge team we are participating in the Plant Workshop at the Giant Jamboree).

To help the Valencia-UPV team we sent them our plant-expression constructs for testing their fluorescence-measurement device. We also received parts from them, which we tested in the plant chassis, Nicotiana, in a transient assay. We recorded the result using confocal microscopy. Finally, we sent them the ‘MoClo Flipper Constructs’ made by the 2014 NRP-UEA tallowing them to easily convert their GoldenGate parts to standardised BioBricks.

You can learn more by clicking on the image on the right.

TU_Eindhoven team

The The Tu-Eindhoven are compiling ‘Cloning Guide’ for future iGEM teams. We have contributed a chapter on Golden Gate Cloning, which we use for the assembly of parts into plant expression vectors. The guide can be found on team Eindhoven’s wiki: https://2015.igem.org/Team:TU_Eindhoven/Collaborations/CloningGuide

You can learn more by clicking on the image on the right.