Difference between revisions of "Team:Northeastern Boston/BasicParts"
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<h2>Hygromycin B Resistance Cassette</h2> | <h2>Hygromycin B Resistance Cassette</h2> | ||
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<p>Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from <a href="http://chlamycollection.org/plasmid/popt_mvenus_hyg" target="_blank">pOpt_mVenus_Hyg</a> and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any <i>Chlamydomonas reinhardtii</i> transformed with this part.</p> | <p>Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from <a href="http://chlamycollection.org/plasmid/popt_mvenus_hyg" target="_blank">pOpt_mVenus_Hyg</a> and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any <i>Chlamydomonas reinhardtii</i> transformed with this part.</p> | ||
Revision as of 17:12, 14 September 2015
BASIC & COMPOSITE PARTS
Hygromycin B Resistance Cassette
Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from pOpt_mVenus_Hyg and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transformed with this part.
This part contains intron 2 of RBCS2 after a fusion HSP70A-RBCS2 promoter. [1] Aph7'', a phosphotransferase, confers resistance to Hygromycin B at agar antibiotic concentrations up to 200g/mL. [2]
mVenus Fluorescent Protein
This part contains mVenus, a nuclear codon-optimized YFP variant (excitation/emission: 515/528nm) [3]. It has Intron 1 and 2 of RBCS2 for increased expression. This is the first independent fluorescent protein codon-optimized for Chlamydomonas reinhardtii submitted to the iGEM registry.