Difference between revisions of "Team:Pitt/Protocols"

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<a href="#bacterial-protocols">Bacterial protocols</a>\
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<a href="#competent-cell-preparation">Preparation of chemically competent cells</a>\
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<a href='#competent-cell-preparation'>Preparation of chemically competent cells</a>\
 
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<a href="#transformation">Chemical transformation of competent cells</a>\
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<a href='#transformation'>Chemical transformation of competent cells</a>\
 
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<a href="#small-culture-preparation">Preparation of 3-5 mL cell culture</a>\
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<a href='#small-culture-preparation'>Preparation of 3-5 mL cell culture</a>\
 
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<a href="#large-culture-preparation">Preparation of 100-1000 mL cell culture</a>\
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<a href='#large-culture-preparation'>Preparation of 100-1000 mL cell culture</a>\
 
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<a href="#">Preparation of </a>\
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<a href='#'>Preparation of </a>\
 
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<h2 id="bacterial-protocols">Bacterial protocols</h2>
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<h3>Preparation of chemically competent cells</h3>
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<h4>This protocol is used to prepare 50 eppendorf tubes, each with 200 &mu;L of cells that can be transformed with the <a href="#transformation>transformation</a> protocol below.
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Revision as of 04:17, 17 June 2015

Bacterial protocols

Preparation of chemically competent cells

This protocol is used to prepare 50 eppendorf tubes, each with 200 μL of cells that can be transformed with the