Difference between revisions of "Team:LASATX/Experiments"
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<li> Experiments </li> | <li> Experiments </li> | ||
<li>Documentation of the development of your project </li> | <li>Documentation of the development of your project </li> | ||
| − | </ul | + | </ul> |
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li> | <li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li> | ||
</ul> | </ul> | ||
| − | </div> | + | </div> --> |
| + | |||
| + | <h2> Sequence Verification: GIBSON </h2> | ||
| + | <h3> STEP 1: PCR amplification (frag into cloning vector) </h3> | ||
| + | <h3> STEP 2A: gel purification </h3> | ||
| + | <h3> STEP 2B: gel electrophoresis & PCR clean-up </h3> | ||
| + | <h3> STEP 3: Gibson reaction </h3> | ||
| + | <h3> STEP 4: Chemical Transformation </h3> | ||
| + | <h3> STEP 5: ON cultures </h3> | ||
| + | <h3> STEP 6: Miniprep </h3> | ||
| + | <h3> STEP 7: Sequencing: sample submission </h3> | ||
| + | <h3> STEP 8: Analyzing sequences </h3> | ||
| + | |||
| + | <h2> Sequence Verification: TOPO-TA </h2> | ||
| + | |||
| + | <h2> pchBA Genomic Extraction </h2> | ||
</html> | </html> | ||
Revision as of 22:45, 17 June 2015
