Difference between revisions of "Team:Nankai/Parts"

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   <tr>
 
   <tr>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628001">BBa_K1628001</a></td>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628001">BBa_K1628001</a></td>
−
     <td bgcolor="#CCCCCC"><span class="zaq">Pbca</span></td>
+
     <td bgcolor="#CCCCCC"><span class="zaq">P<sub>bca</sub></span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Promoter</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Promoter</span></td>
−
     <td bgcolor="#CCCCCC"><span class="zaq"> an original promoter of coding sequence pgsBCA</span></td>
+
     <td bgcolor="#CCCCCC"><span class="zaq"> an original promoter of coding sequence <em>pgsBCA</em> operon</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Tianyi Huang</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Tianyi Huang</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">364</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">364</span></td>
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   <tr>
 
   <tr>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002">BBa_K1628002</a></td>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002">BBa_K1628002</a></td>
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     <td bgcolor="#FFFFFF"><span class="zaq">SPxyl </span></td>
+
     <td bgcolor="#FFFFFF"><span class="zaq">P<sub>xyl</sub></span></td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">Promoter</span></td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">Promoter</span></td>
−
     <td bgcolor="#FFFFFF">&nbsp;</td>
+
     <td bgcolor="#FFFFFF">a promoter of xylose operon</td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">Tianyi Huang</span></td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">Tianyi Huang</span></td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">217</span></td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">217</span></td>
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     <td bgcolor="#CCCCCC"><span class="zaq">BJ27UP</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">BJ27UP</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Promoter</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Promoter</span></td>
−
     <td bgcolor="#CCCCCC"><span class="zaq">a synthetic promoter</span></td>
+
     <td bgcolor="#CCCCCC">an artificially synthesized promoter</td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Xinhao Song</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Xinhao Song</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">100</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">100</span></td>
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     <td bgcolor="#FFFFFF"><span class="zaq">C2up</span></td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">C2up</span></td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">Promoter</span></td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">Promoter</span></td>
−
     <td bgcolor="#FFFFFF">&nbsp;</td>
+
     <td bgcolor="#FFFFFF">an artificially synthesized promoter</td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">Xinhao Song</span></td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">Xinhao Song</span></td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">108</span></td>
 
     <td bgcolor="#FFFFFF"><span class="zaq">108</span></td>
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   <tr>
 
   <tr>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628005">BBa_K1628005</a></td>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628005">BBa_K1628005</a></td>
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     <td bgcolor="#CCCCCC"><span class="zaq">A2cup</span></td>
+
     <td bgcolor="#CCCCCC"><span class="zaq">A2up</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Promoter</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Promoter</span></td>
−
     <td bgcolor="#CCCCCC">&nbsp;</td>
+
     <td bgcolor="#CCCCCC">an artificially synthesized  promoter</td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Yibing Wei</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Yibing Wei</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">107</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">107</span></td>
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     <td><span class="zaq">P43</span></td>
 
     <td><span class="zaq">P43</span></td>
 
     <td><span class="zaq">Promoter</span></td>
 
     <td><span class="zaq">Promoter</span></td>
−
     <td>&nbsp;</td>
+
     <td>a strong promoter in <em>Bacillus subtilis </em>168</td>
 
     <td><span class="zaq">Yibing Wei</span></td>
 
     <td><span class="zaq">Yibing Wei</span></td>
 
     <td><span class="zaq">445</span></td>
 
     <td><span class="zaq">445</span></td>
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   <tr>
 
   <tr>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628007">BBa_K1628007</a></td>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628007">BBa_K1628007</a></td>
−
     <td bgcolor="#CCCCCC"><span class="zaq">PamyA</span></td>
+
     <td bgcolor="#CCCCCC"><span class="zaq">P<sub>amyA</sub></span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Promoter</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Promoter</span></td>
−
     <td bgcolor="#CCCCCC">&nbsp;</td>
+
     <td bgcolor="#CCCCCC">a strong promoter in <em>Bacillus amyloliquefaciens </em>LL3</td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Zhaoran Zhang</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Zhaoran Zhang</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">613</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">613</span></td>
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   <tr bgcolor="#FFFFFF">
 
   <tr bgcolor="#FFFFFF">
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628101">BBa_K1628101</a></td>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628101">BBa_K1628101</a></td>
−
     <td><span class="zaq">pgsB</span></td>
+
     <td><span class="zaq"><em>pgsB</em></span></td>
 
     <td><span class="zaq">Coding</span></td>
 
     <td><span class="zaq">Coding</span></td>
−
     <td>&nbsp;</td>
+
     <td>a gene responsible for  γ-PGA synthesis in <em>pgsBCA</em> operon</td>
 
     <td><span class="zaq">Tianyi Huang</span></td>
 
     <td><span class="zaq">Tianyi Huang</span></td>
 
     <td><span class="zaq">1182</span></td>
 
     <td><span class="zaq">1182</span></td>
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   <tr>
 
   <tr>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628102">BBa_K1628102</a></td>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628102">BBa_K1628102</a></td>
−
     <td bgcolor="#CCCCCC"><span class="zaq">pgsCA</span></td>
+
     <td bgcolor="#CCCCCC"><span class="zaq"><em>pgsCA</em></span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Coding</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Coding</span></td>
−
     <td bgcolor="#CCCCCC">&nbsp;</td>
+
     <td bgcolor="#CCCCCC">a coding gene in <em>pgsBCA</em> operon</td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Xinhao Song</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Xinhao Song</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">1631</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">1631</span></td>
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   <tr bgcolor="#FFFFFF">
 
   <tr bgcolor="#FFFFFF">
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628201">BBa_K1628201</a></td>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628201">BBa_K1628201</a></td>
−
     <td><span class="zaq">PlacI/lacI</span></td>
+
     <td><span class="zaq">P<sub>lacI</sub>/lacI</span></td>
 
     <td><span class="zaq">Translational Unit </span></td>
 
     <td><span class="zaq">Translational Unit </span></td>
−
     <td>&nbsp;</td>
+
     <td>a promoter of lactose operon along with a repressor of lactose operon regulating the promoter</td>
 
     <td><span class="zaq">Yibing Wei</span></td>
 
     <td><span class="zaq">Yibing Wei</span></td>
 
     <td><span class="zaq">1386</span></td>
 
     <td><span class="zaq">1386</span></td>
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   <tr>
 
   <tr>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628202">BBa_K1628202</a></td>
 
     <td bgcolor="#CCCCCC"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628202">BBa_K1628202</a></td>
−
     <td bgcolor="#CCCCCC"><span class="zaq">Pgrac</span></td>
+
     <td bgcolor="#CCCCCC"><span class="zaq">P<sub>grac</sub></span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Promoter </span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Promoter </span></td>
−
     <td bgcolor="#CCCCCC">&nbsp;</td>
+
     <td bgcolor="#CCCCCC">a promoter of lactose operon regulated by repressor LacI</td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Tianyi Huang</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Tianyi Huang</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">120</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">120</span></td>
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     <td><span class="zaq">xylR</span></td>
 
     <td><span class="zaq">xylR</span></td>
 
     <td><span class="zaq">Coding </span></td>
 
     <td><span class="zaq">Coding </span></td>
−
     <td>&nbsp;</td>
+
     <td>a repressor of xylose operon regulating promoter <span class="zaq">P<sub>xyl</sub></span></td>
 
     <td><span class="zaq">Zhaoran Zhang</span></td>
 
     <td><span class="zaq">Zhaoran Zhang</span></td>
 
     <td><span class="zaq">1167</span></td>
 
     <td><span class="zaq">1167</span></td>
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     <td bgcolor="#CCCCCC"><span class="zaq">P1-GFP</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">P1-GFP</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Device</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Device</span></td>
−
     <td bgcolor="#CCCCCC">&nbsp;</td>
+
     <td bgcolor="#CCCCCC">P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry.</td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Tianyi Huang</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Tianyi Huang</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">?</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">?</span></td>
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     <td><span class="zaq">P2-GFP</span></td>
 
     <td><span class="zaq">P2-GFP</span></td>
 
     <td><span class="zaq">Device</span></td>
 
     <td><span class="zaq">Device</span></td>
−
     <td>&nbsp;</td>
+
     <td>P2-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23106, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into <em>E. coli</em> cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry.</td>
 
     <td><span class="zaq">Xinhao Song</span></td>
 
     <td><span class="zaq">Xinhao Song</span></td>
 
     <td><span class="zaq">?</span></td>
 
     <td><span class="zaq">?</span></td>
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     <td bgcolor="#CCCCCC"><span class="zaq">P3-GFP </span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">P3-GFP </span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Device</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Device</span></td>
−
     <td bgcolor="#CCCCCC">&nbsp;</td>
+
     <td bgcolor="#CCCCCC">P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into <em>E. coli </em>cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry.</td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Yibing Wei</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">Yibing Wei</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">?</span></td>
 
     <td bgcolor="#CCCCCC"><span class="zaq">?</span></td>
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<h6>References</h6>
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<h6>&nbsp;</h6>
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<p>1. Ashiuchi, M., Misono, H., 2002. Biochemistry and molecular genetics of poly-γ-glutamate synthesis. Appl. Biochem. Biotechnol. 59, 9–14.</br>
+
−
2. Kunioka, M., 1997. Biosynthesis and chemical reactions of poly(amino acid)s from
+
−
microorganisms. Appl. Microbiol. Biotechnol. 47, 469–475.</br>
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−
3. Shih, I.L., Van, Y.T., 2001. The production of poly(γ-glutamic acid) from microorganism and its various applications. Bioresour. Technol. 79, 207–225.</br>
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4. Li, C., 2002. Poly(L-glutamic acid)--anticancer drug conjugates. Adv. Drug Deliver. Rev. 54, 695–713.</br>
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5. Liang, H.F., Chen, C.T., Chen, S.C., Kulkarni, A.R., Chiu, Y.L., Chen, M.C., Sung, H.W., 2006. Paclitaxel-loaded poly(γ-glutamic acid)-poly(lactide) nanoparticles as a targeted drug delivery system for the treatment of liver cancer. Biomaterials. 27, 2051–2059.</br>
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6. Richard, A., Margaritis, A., 2001. Poly (glutamic acid) for biomedical applications. Crit. Rev. Biotechnol. 21, 219–232.</br>
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7. Park, Y.J., Liang, J., Yang, Z., Yang, V.C., 2001. Controlled release of clot-dissolving tissue-type plasmmogen activator from a poly(L-glutamic acid) semi-interpenetrating polymer network hydrogel. J. Control. Release. 74, 243–247.</br>
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8. Cao, M.F., Geng, W.T., Liu, L., Song, C.J., Xie, H., Guo, W.B., Jin, Y.H., Wang, S.F., 2011. Glutamic acid independent production of poly-γ-glutamic acid by Bacillus amyloliquefaciens LL3 and cloning of pgsBCA genes. Bioresour. Technol. 102, 4251–4257.</br>
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9. Geng, W.T., Cao, M.F., Song, C.J., Xie, H., Liu, L., Yang, C., Feng, J., Zhang, W., Jin, Y.H., Du, Y., Wang, S.F., 2011. Complete genome sequence of Bacillus amyloliquefaciens LL3, which exhibits glutamic acid-independent production of poly-γ-glutamic acid. J. Bacteriol. 193, 3393–3394.</br>
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10. Feng, J., Gao, W.X., Gu, Y.Y., Zhang, W., Cao, M.F., Song, C.J., Zhang, P., Sun, M., Yang, C.,  Wang, S.F., 2014a. Functions of poly-gamma-glutamic acid (γ-PGA) degradation genes in γ-PGA synthesis and cell morphology maintenance. Appl. Microbiol. Biotechnol. 98, 6397–6407.</br>
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11. Uy, D., Delaunay S., Germain, P., Engasser, J.M., Goergen, J.L. 2003. Instability of glutamate production by Corynebacterium glutamicum 2262 in continuous culture using the temperature-triggered process. J. Biotech. 104, 173-184.</p>
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Team Parts

Parts Abstract

Number Name Type Description Creator Length
BBa_K1628001 Pbca Promoter an original promoter of coding sequence pgsBCA operon Tianyi Huang 364
BBa_K1628002 Pxyl Promoter a promoter of xylose operon Tianyi Huang 217
BBa_K1628003 BJ27UP Promoter an artificially synthesized promoter Xinhao Song 100
BBa_K1628004 C2up Promoter an artificially synthesized promoter Xinhao Song 108
BBa_K1628005 A2up Promoter an artificially synthesized promoter Yibing Wei 107
BBa_K1628006 P43 Promoter a strong promoter in Bacillus subtilis 168 Yibing Wei 445
BBa_K1628007 PamyA Promoter a strong promoter in Bacillus amyloliquefaciens LL3 Zhaoran Zhang 613
BBa_K1628101 pgsB Coding a gene responsible for γ-PGA synthesis in pgsBCA operon Tianyi Huang 1182
BBa_K1628102 pgsCA Coding a coding gene in pgsBCA operon Xinhao Song 1631
BBa_K1628201 PlacI/lacI Translational Unit a promoter of lactose operon along with a repressor of lactose operon regulating the promoter Yibing Wei 1386
BBa_K1628202 Pgrac Promoter a promoter of lactose operon regulated by repressor LacI Tianyi Huang 120
BBa_K1628203 xylR Coding a repressor of xylose operon regulating promoter Pxyl Zhaoran Zhang 1167
BBa_K1628301 P1-GFP Device P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry. Tianyi Huang ?
BBa_K1628202 P2-GFP Device P2-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23106, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry. Xinhao Song ?
BBa_K1628303 P3-GFP Device P3-GFP is a composite part used for the Measurement track of this year. This part consists of the promoter part of BBa_J23117, the official GFP device (with an RBS, GFP coding sequence and a set of double terminators) and a pSB1C3 backbone. We transformed this plasmid into E. coli cells and the fluorescence intensity of the GFP protein that was expressed by the bacteria was measured afterwards by a flow cytometry. Yibing Wei ?

 

 

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