Difference between revisions of "Team:Nankai/Composite Part"

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<div class="col-md-8 blog-posts">
 
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<h4>Composite Parts</h4>
 
<h4>Composite Parts</h4>
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<p style="position: relative; top:0px; left: 20px; width:700px;  font-size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify; line-height:30px;"><b>Composite part example 1 </b></br>
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<p style="position: relative; top:0px; left: 20px; width:700px;  font-size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify; line-height:30px;"> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628201">BBa_K1628201</a> (P<sub>lacI</sub>/lacI) </p>
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  （consists of basic part1 and basic part2）<a href="http://partsregistry.org/Part:BBa_K1031221">BBa_K1031221</a>, <a href="http://partsregistry.org/Part:BBa_K1031222">BBa_K1031222</a>, <a href="http://partsregistry.org/Part:BBa_K1031223">BBa_K1031223</a> and <a href="http://partsregistry.org/Part:BBa_K1031224">BBa_K1031224</a> are the DmpR biosensors using different RBS preceding sfGFP. </br>
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<p style="position: relative; top:0px; left: 20px; width:700px;  font-size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify; line-height:30px;">Promoter P<sub>lacI</sub> is a promoter of lactose operon and LacI is a repressor of lactose operon regulating promoter P<sub>lacI</sub>. PlacI is the native promoter of LacI. PlacI and LacI together with promoter P<sub>xyl</sub> (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628002">BBa_K1628002</a>), promoter P<sub>grac</sub> (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628202">BBa_K1628202</a>) and repressor XylR (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628203">BBa_K1628203</a>) formed a metabolic toggle switch. We used this device to regulate the expression of <em>odhAB</em> genes in <em>Bacillus amyloliquefaciens</em> NK-1 (showed in Figure 2). Without IPTG, the promoter P<sub>grac</sub> is inhibited by suppressor LacI and the supreessor XylR will not synthesized, thus the promoter P<sub>xyl</sub> is active and <em>odhAB</em> genes are expressed. When IPTG is added, the xylR gene is expressed and the suppressor XylR is synthesized thereafter inhibited the expression of odhAB genes.<br/>
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  <a href="http://partsregistry.org/Part:BBa_K1031211">BBa_K1031211</a> is the DmpR transcriptional factor under a constitutive promoter.description  description  description  description  description  description  description  description  description  description  description  description  description  <br/>
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</p>
 
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<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<p style="position: relative; top:0px; left: 20px; width:700px;  font-size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify; line-height:30px;"><b>Composite part example 2 </b></br>
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<p style="position: relative; top:0px; left: 20px; width:700px;  font-size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify; line-height:30px;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628202">BBa_K1628202</a> (P<sub>grac</sub>)</p>
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（consists of basic part3 and basic part4）<a href="http://partsregistry.org/Part:BBa_K1031221">BBa_K1031221</a>, <a href="http://partsregistry.org/Part:BBa_K1031222">BBa_K1031222</a>, <a href="http://partsregistry.org/Part:BBa_K1031223">BBa_K1031223</a> and <a href="http://partsregistry.org/Part:BBa_K1031224">BBa_K1031224</a> are the DmpR biosensors using different RBS preceding sfGFP. </br>
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<p style="position: relative; top:0px; left: 20px; width:700px;  font-size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify; line-height:30px;">Promoter P<sub>grac</sub> is a promoter of lactose operon regulated by repressor LacI. Pgrac together with promoter P<sub>lacI</sub>, repressor LacI (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628201">BBa_K1628201</a>), promoter P<sub>xyl</sub> (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628202">BBa_K1628202</a>) and repressor XylR (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1628203">BBa_K1628203</a>) formed a metabolic toggle switch. We used this device to regulate the expression of <em>odhAB</em> genes in <em>Bacillus amyloliquefaciens</em> NK-1 (showed in Figure 2) Without IPTG, the promoter P<sub>grac</sub> is inhibited by suppressor LacI and the supreessor XylR will not synthesized, thus the promoter P<sub>xyl</sub> is active and <em>odhAB</em> genes are expressed. When IPTG is added, the<em> xylR</em> gene is expressed and the suppressor XylR is synthesized thereafter inhibited the expression of <em>odhAB </em>genes.</p>
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  <a href="http://partsregistry.org/Part:BBa_K1031211">BBa_K1031211</a> is the DmpR transcriptional factor under a constitutive promoter.description  description  description  description  description  description  description  description  description  description  description  description  description <br/>
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<p><span class="sidebar-widget"><img src="https://static.igem.org/mediawiki/2015/f/f0/Parts_figure2.jpeg" /></span></p>
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</p>
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<p style="position: relative; top: 0px; left: 20px; width: 700px; font-size: 18px; font-family: calibri,Arial, Helvetica, sans-serif; text-align: justify; line-height: 30px;">Figure 2. Metabolic toggle switch to regulate the expression of <em>odhAB</em></p>
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<p><span class="sidebar-widget"><img src="https://static.igem.org/mediawiki/2015/f/f2/Nankai_projectpic3.JPG" alt="Basic part picture" /></span></p>
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<p>&nbsp;</p>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<img src="https://static.igem.org/mediawiki/2015/f/f2/Nankai_projectpic3.JPG">
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<img src="https://static.igem.org/mediawiki/2015/d/de/Partsfigure_new1.jpeg">
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                                                 <p>Part-picture-1</p>
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                                                 <p>&nbsp;</p>
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<img src="https://static.igem.org/mediawiki/2015/6/6e/Nankai_projectpic1.JPG">
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<img src="https://static.igem.org/mediawiki/2015/4/40/Partsfigure_new2.jpeg">
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                                                <p>Part-picture-2</p>
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                      <p>&nbsp;</p>
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<img src="https://static.igem.org/mediawiki/2015/2/2d/Nankai_projectpic2.jpg">
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<img src="https://static.igem.org/mediawiki/2015/a/ad/Partsfigure_new3.jpeg">
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<h6>References</h6>
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<p>1. Ashiuchi, M., Misono, H., 2002. Biochemistry and molecular genetics of poly-γ-glutamate synthesis. Appl. Biochem. Biotechnol. 59, 9–14.</br>
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2. Kunioka, M., 1997. Biosynthesis and chemical reactions of poly(amino acid)s from
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microorganisms. Appl. Microbiol. Biotechnol. 47, 469–475.</br>
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3. Shih, I.L., Van, Y.T., 2001. The production of poly(γ-glutamic acid) from microorganism and its various applications. Bioresour. Technol. 79, 207–225.</br>
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4. Li, C., 2002. Poly(L-glutamic acid)--anticancer drug conjugates. Adv. Drug Deliver. Rev. 54, 695–713.</br>
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5. Liang, H.F., Chen, C.T., Chen, S.C., Kulkarni, A.R., Chiu, Y.L., Chen, M.C., Sung, H.W., 2006. Paclitaxel-loaded poly(γ-glutamic acid)-poly(lactide) nanoparticles as a targeted drug delivery system for the treatment of liver cancer. Biomaterials. 27, 2051–2059.</br>
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6. Richard, A., Margaritis, A., 2001. Poly (glutamic acid) for biomedical applications. Crit. Rev. Biotechnol. 21, 219–232.</br>
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7. Park, Y.J., Liang, J., Yang, Z., Yang, V.C., 2001. Controlled release of clot-dissolving tissue-type plasmmogen activator from a poly(L-glutamic acid) semi-interpenetrating polymer network hydrogel. J. Control. Release. 74, 243–247.</br>
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8. Cao, M.F., Geng, W.T., Liu, L., Song, C.J., Xie, H., Guo, W.B., Jin, Y.H., Wang, S.F., 2011. Glutamic acid independent production of poly-γ-glutamic acid by Bacillus amyloliquefaciens LL3 and cloning of pgsBCA genes. Bioresour. Technol. 102, 4251–4257.</br>
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9. Geng, W.T., Cao, M.F., Song, C.J., Xie, H., Liu, L., Yang, C., Feng, J., Zhang, W., Jin, Y.H., Du, Y., Wang, S.F., 2011. Complete genome sequence of Bacillus amyloliquefaciens LL3, which exhibits glutamic acid-independent production of poly-γ-glutamic acid. J. Bacteriol. 193, 3393–3394.</br>
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10. Feng, J., Gao, W.X., Gu, Y.Y., Zhang, W., Cao, M.F., Song, C.J., Zhang, P., Sun, M., Yang, C.,  Wang, S.F., 2014a. Functions of poly-gamma-glutamic acid (γ-PGA) degradation genes in γ-PGA synthesis and cell morphology maintenance. Appl. Microbiol. Biotechnol. 98, 6397–6407.</br>
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11. Uy, D., Delaunay S., Germain, P., Engasser, J.M., Goergen, J.L. 2003. Instability of glutamate production by Corynebacterium glutamicum 2262 in continuous culture using the temperature-triggered process. J. Biotech. 104, 173-184.</p>
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Revision as of 13:38, 15 September 2015

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Team Parts

Composite Parts

BBa_K1628201 (PlacI/lacI)

Promoter PlacI is a promoter of lactose operon and LacI is a repressor of lactose operon regulating promoter PlacI. PlacI is the native promoter of LacI. PlacI and LacI together with promoter Pxyl (BBa_K1628002), promoter Pgrac (BBa_K1628202) and repressor XylR (BBa_K1628203) formed a metabolic toggle switch. We used this device to regulate the expression of odhAB genes in Bacillus amyloliquefaciens NK-1 (showed in Figure 2). Without IPTG, the promoter Pgrac is inhibited by suppressor LacI and the supreessor XylR will not synthesized, thus the promoter Pxyl is active and odhAB genes are expressed. When IPTG is added, the xylR gene is expressed and the suppressor XylR is synthesized thereafter inhibited the expression of odhAB genes.

 

BBa_K1628202 (Pgrac)

Promoter Pgrac is a promoter of lactose operon regulated by repressor LacI. Pgrac together with promoter PlacI, repressor LacI (BBa_K1628201), promoter Pxyl (BBa_K1628202) and repressor XylR (BBa_K1628203) formed a metabolic toggle switch. We used this device to regulate the expression of odhAB genes in Bacillus amyloliquefaciens NK-1 (showed in Figure 2) Without IPTG, the promoter Pgrac is inhibited by suppressor LacI and the supreessor XylR will not synthesized, thus the promoter Pxyl is active and odhAB genes are expressed. When IPTG is added, the xylR gene is expressed and the suppressor XylR is synthesized thereafter inhibited the expression of odhAB genes.

Figure 2. Metabolic toggle switch to regulate the expression of odhAB

 

 

 

 

 

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