Difference between revisions of "Team:ZJU-China/Design/Toxinmanufacture"

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<div>
 
<div>
−
<p class="p1">We listed 23 pieces of Breaking News. Here are the links:
+
<p class="p1" style="font-size:20px;">We listed 23 pieces of Breaking News. Here are the links:
 
<ul>
 
<ul>
−
<li>Successfully got the solution of CNC!! And it appeared Tyndall effect.
+
<a href="#event1  "><li>  15 members was picked out. ZJU-China 2015 team was set up~ Our story began.
−
</li><li>Successfully checked in termites and tc protein family.
+
</li></a> <a href="#e1  " ><li> 1st Group meeting. Everyone was assigned with his/her own mission. Brainstorm began.
−
</li><li>Got the genome of tcdA1 and tcdB1 in NCBI. Got TT01(a species of Photorhabdus luminescens) which could produce toxic protein. It would play an important role in our plan B.
+
</li></a> <a href="#e2  " ><li> Team registration completed and handed in team registration fee.
−
</li><li>Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio.
+
</li></a> <a href="#e3  " ><li> 11th Group meeting. Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project.
−
</li><li>Got the E.coli strain ET12567 from Professor Li's Lab. Began the experiment about Streptomyces avermitilis.
+
</li></a> <a href="#e4  " ><li> Successfully got the solution of CNC!! And it appeared Tyndall effect.
−
</li><li>iGEM Shanghai Tour. Took part in the conference held in NYU. Visited FDU and communicated with them.
+
</li></a> <a href="#e5  " ><li> Successfully checked in termites and tc protein family.
−
</li><li>Got the solid CNC with lyophilization. Built the framework of the first web page.
+
</li></a> <a href="#e6  " ><li> Got the genome of tcdA1 and tcdB1 in NCBI. Got TT01(a species of Photorhabdus luminescens) which could produce toxic protein. It would play an important role in our plan B.
−
</li><li>Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE!  Cheers~
+
</li></a> <a href="#e7  " ><li> Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio.
−
</li><li>Judge Haoqian Zhang from Peking University came to our lab. Frozen the spores of Streptomyces avermitilis.
+
</li></a> <a href="#e8  " ><li> Got the E.coli strain ET12567 from Professor Li's Lab. Began the experiment about Streptomyces avermitilis.
−
</li><li>Went to shanghai for the interview of visa. 12 members successfully passed the interview.  
+
</li></a> <a href="#e9  " ><li> iGEM Shanghai Tour. Took part in the conference held in NYU. Visited FDU and communicated with them.
−
</li><li>Solved the diffusion equation.
+
</li></a> <a href="#e10  " ><li> Got the solid CNC with lyophilization. Built the framework of the first web page.
−
</li><li>Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST.  
+
</li></a> <a href="#e11  " ><li> Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE!  Cheers~
−
</li><li>Discussed helping BNU and HUST. Designed and alpha tested the Synbio-cards. Improved the modeling.
+
</li></a> <a href="#e12  " ><li> Judge Haoqian Zhang from Peking University came to our lab. Frozen the spores of Streptomyces avermitilis.
−
</li><li>Giant Jamboree registration completed.
+
</li></a> <a href="#e13  " ><li> Went to shanghai for the interview of visa. 12 members successfully passed the interview.  
−
</li><li>34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety.
+
</li></a> <a href="#e14  " ><li> Solved the diffusion equation.
−
</li><li>Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).
+
</li></a> <a href="#e15  " ><li> Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST.  
−
</li><li>Completed our framework and philosophy of safety.
+
</li></a> <a href="#e16  " ><li> Discussed helping BNU and HUST. Designed and alpha tested the Synbio-cards. Improved the modeling.
−
</li><li>Bacteria solution PCR: device-plu1537 and CDS-plu1537. The sequence of ermE+plasmid, Frr+plasmid and plu1537+plasmid were confirmed, which marked the success in standardization of 3 parts!!
+
</li></a> <a href="#e17  " ><li> Giant Jamboree registration completed.
−
</li><li>Got our device for detecting and killing termites.  
+
</li></a> <a href="#e18  " ><li> 34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety.
−
</li>
+
</li></a> <a href="#e19  " ><li> Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).
−
 
+
</li></a> <a href="#e20  " ><li> Completed our framework and philosophy of safety.
 +
</li></a> <a href="#e21  " ><li> Bacteria solution PCR: device-plu1537 and CDS-plu1537. The sequence of ermE+plasmid, Frr+plasmid and plu1537+plasmid were confirmed, which marked the success in standardization of 3 parts!!
 +
</li></a> <a href="#e22  " ><li> Got our device for detecting and killing termites.  
 +
</li></a> <a href="#e23  " ><li> 
 +
</li></a>                         
 +
                                 
 
</p>
 
</p>
 
</ul>
 
</ul>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Seamless assembly: plasmid+OrfX, plasmid+metK. PCR: CDS-tcdA1. Double enzyme digestion: RFP+plasmid. Seamless assembly: CDS-plu0840. PCR and DNA gel extraction: CDS-tcdA1. Changed pSB1A3 to pSB1C3: plu0840, plu1537, tcdA1. Transformed them into E.coli DH5α, selected from single colonies and used single enzyme digestion to verify the got plasmids.</p>
+
<p id="e23">Seamless assembly: plasmid+OrfX, plasmid+metK. PCR: CDS-tcdA1. Double enzyme digestion: RFP+plasmid. Seamless assembly: CDS-plu0840. PCR and DNA gel extraction: CDS-tcdA1. Changed pSB1A3 to pSB1C3: plu0840, plu1537, tcdA1. Transformed them into E.coli DH5α, selected from single colonies and used single enzyme digestion to verify the got plasmids.</p>
 
</div>
 
</div>
 
</div>
 
</div>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>The sequence of ermE+plasmid, Frr+plasmid and plu1537+plasmid were confirmed, which marked the success in standardization of 3 parts!!</p>
+
<p id="e22">The sequence of ermE+plasmid, Frr+plasmid and plu1537+plasmid were confirmed, which marked the success in standardization of 3 parts!!</p>
 
</div>
 
</div>
 
</div>
 
</div>
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<ul>
 
<ul>
 
<li><p class="p1">PCR: tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R and plu1537.</p></li>
 
<li><p class="p1">PCR: tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R and plu1537.</p></li>
−
<li><p class="p1">Completed our framework and philosophy of safety.</p></li>
+
<li><p class="p1" id = "e21">Completed our framework and philosophy of safety.</p></li>
 
<li><p class="p1">Extracted the plasmid containing RFP from DH5α. PCR: RFP and confirmed by gel electrophoresis.</p></li>
 
<li><p class="p1">Extracted the plasmid containing RFP from DH5α. PCR: RFP and confirmed by gel electrophoresis.</p></li>
 
</ul>
 
</ul>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).</p>
+
<p id="e20">Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/b/bb/8-8-3.jpg/800px-8-8-3.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/b/bb/8-8-3.jpg/800px-8-8-3.jpg" class="img-responsive">
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety.</p>
+
<p id="e19">34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety.</p>
 
</div>
 
</div>
 
</div>
 
</div>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Giant Jamboree registration completed.</p>
+
<p id = "e18">Giant Jamboree registration completed.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/e/e5/7-28.png/420px-7-28.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/e/e5/7-28.png/420px-7-28.png" class="img-responsive">
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Discussed helping BNU and HUST. Designed and alpha tested the Synbio-cards. Improved the modeling.</p>
+
<p id="e17">Discussed helping BNU and HUST. Designed and alpha tested the Synbio-cards. Improved the modeling.</p>
 
</div>
 
</div>
 
</div>
 
</div>
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<p><small class="text-muted">7.19.2015-7.24.2015</small></p>
 
<p><small class="text-muted">7.19.2015-7.24.2015</small></p>
 
</div>
 
</div>
−
<div class="tl-body">
+
<div class="tl-body" id = "e16">
 
<p>Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST. </p>
 
<p>Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST. </p>
 
</div>
 
</div>
Line 1,019: Line 1,017:
 
<p><small class="text-muted">6.14.2015</small></p>
 
<p><small class="text-muted">6.14.2015</small></p>
 
</div>
 
</div>
−
<div class="tl-body">Solved the diffusion equation.
+
<div class="tl-body" id="e15">Solved the diffusion equation.
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/3/3d/6-14-2.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/3/3d/6-14-2.jpg" class="img-responsive">
Line 1,044: Line 1,042:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Went to shanghai for the interview of visa. 12 members successfully passed the interview. </p>
+
<p id="e14">Went to shanghai for the interview of visa. 12 members successfully passed the interview. </p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 1,128: Line 1,126:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Frozen the spores of Streptomyces avermitilis.</p>
+
<p id="e13">Frozen the spores of Streptomyces avermitilis.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/03/6-6.jpeg/800px-6-6.jpeg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/03/6-6.jpeg/800px-6-6.jpeg" class="img-responsive">
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<ul>
 
<ul>
 
<li><p class="p1">Discussed the characterization of E.coli embedded in CNC, including TEM, SEM, confocal, FTIR and flow cytometry.</p></li>
 
<li><p class="p1">Discussed the characterization of E.coli embedded in CNC, including TEM, SEM, confocal, FTIR and flow cytometry.</p></li>
−
<li><p class="p1">Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE!  Cheers~</p></li>
+
<li><p class="p1" id ="e12">Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE!  Cheers~</p></li>
 
<li><p class="p1">Brainstormed how to do human practice and use the €5000. We came up with the  Synbio-cards and Synbio-workshops.</p></li>
 
<li><p class="p1">Brainstormed how to do human practice and use the €5000. We came up with the  Synbio-cards and Synbio-workshops.</p></li>
 
</ul>
 
</ul>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Got the solid CNC with lyophilization. Built the framework of the first web page.</p>
+
<p id ="e11">Got the solid CNC with lyophilization. Built the framework of the first web page.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/0b/5-26.png/800px-5-26.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/0b/5-26.png/800px-5-26.png" class="img-responsive">
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>iGEM Shanghai Tour. Took part in the conference held in NYU. Visited FDU and communicated with them.</p>
+
<p id = e10>iGEM Shanghai Tour. Took part in the conference held in NYU. Visited FDU and communicated with them.</p>
 
</div>
 
</div>
 
</div>
 
</div>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Got the E.coli strain ET12567 from Professor Li's Lab. Began the experiment about Streptomyces avermitilis.</p>
+
<p id="e9">Got the E.coli strain ET12567 from Professor Li's Lab. Began the experiment about Streptomyces avermitilis.</p>
 
</div>
 
</div>
 
</div>
 
</div>
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<p><small class="text-muted">5.17.2015</small></p>
 
<p><small class="text-muted">5.17.2015</small></p>
 
</div>
 
</div>
−
<div class="tl-body">Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio.
+
<div class="tl-body" id ="e8">Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio.
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/a/a3/5-17.jpg/800px-5-17.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/a/a3/5-17.jpg/800px-5-17.jpg" class="img-responsive">
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<div class="tl-body">
 
<div class="tl-body">
 
<ul>
 
<ul>
−
<li><p class="p1">Got the genome of tcdA1 and tcdB1 in NCBI.</p></li>
+
<li><p class="p1" id = "e7">Got the genome of tcdA1 and tcdB1 in NCBI.</p></li>
 
<li><p class="p1">Got TT01(a species of Photorhabdus luminescens) which could produce toxic protein. It would play an important role in our plan B.</p></li>
 
<li><p class="p1">Got TT01(a species of Photorhabdus luminescens) which could produce toxic protein. It would play an important role in our plan B.</p></li>
 
</ul>
 
</ul>
Line 1,478: Line 1,476:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Successfully checked in termites and tc protein family.</p>
+
<p id = "e6">Successfully checked in termites and tc protein family.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/0/02/5-12.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/0/02/5-12.png" class="img-responsive">
Line 1,527: Line 1,525:
 
</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p class="p1">Successfully got the solution of CNC!! And it appeared Tyndall effect.</p>
+
<p class="p1" id = "e5">Successfully got the solution of CNC!! And it appeared Tyndall effect.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/c/c1/5-6.jpg/800px-5-6.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/c/c1/5-6.jpg/800px-5-6.jpg" class="img-responsive">
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>11th Group meeting. Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project. </p>
+
<p id = "e4">11th Group meeting. Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project. </p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/c/c0/4-19.jpg/800px-4-19.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/c/c0/4-19.jpg/800px-4-19.jpg" class="img-responsive">
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>Team application completed.</p>
+
<p id = e3>Team application completed.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/04/3-25.png/431px-3-25.png" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/thumb/0/04/3-25.png/431px-3-25.png" class="img-responsive">
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>1st Group meeting. Everyone was assigned with his/her own mission. Brainstorm began.</p>
+
<p id="e2">1st Group meeting. Everyone was assigned with his/her own mission. Brainstorm began.</p>
 
</div>
 
</div>
 
</div>
 
</div>
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</div>
 
</div>
 
<div class="tl-body">
 
<div class="tl-body">
−
<p>15 members was picked out. ZJU-China 2015 team was set up~ Our story began.</p>
+
<p id ="event1">15 members was picked out. ZJU-China 2015 team was set up~ Our story began.</p>
 
</div>
 
</div>
 
<img src="https://static.igem.org/mediawiki/2015/c/c8/1-30-2.jpg" class="img-responsive">
 
<img src="https://static.igem.org/mediawiki/2015/c/c8/1-30-2.jpg" class="img-responsive">

Revision as of 17:05, 15 September 2015

Notebook

We listed 23 pieces of Breaking News. Here are the links:

  • September

  • 9.5.2015

    Finished the overview of entire project.

  • 9.4.2015

    • Bacteria solution PCR: OrfX. One positive clone of 20 samples.

    • DNA sequencing: OrfX.

  • 9.2.2015

    Dynamic Light Scattering(DLS) experiment to measure the particle size of embedded E.coli.

  • 9.1.2015

    The sequence of OrfX was not correct because of the wrong template, thus we had to do the related experiments again.

  • August

  • 8.31.2015

    Took photos and prepared for the banner~

  • 8.31.2015

    Took photos and prepared for the banner~

  • 8.30.2015

    The sequence of metK and tcdA1 were confirmed, which marked the success in standardization of 2 parts!!

  • 8.29.2015

    Bacteria solution PCR: metK and OrfX. Both had positive clones. DNA sequencing: metK and OrfX. T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3. Selected from single colonies: CDS-tcdA1.

  • 8.28.2015

    • Selected from single colonies of metK and OrfX.

    • Submit the safety form. Selected from single colonies: CDS-plu0840. Double enzyme digestion and DNA gel extraction: plu1537, tcdA1, plu0840. Plasmid extration and double enzyme digestion: RFP. Protein extraction and SDS-PAGE: tcdA1, plu1537, plu0840. T4 ligation and transformation: plu0840+pSB1C3, plu1537+pSB1C3, tcdA1+pSB1C3. Bacteria solution PCR: CDS-plu0840.

  • 8.27.2015

    Seamless assembly: plasmid+OrfX, plasmid+metK. PCR: CDS-tcdA1. Double enzyme digestion: RFP+plasmid. Seamless assembly: CDS-plu0840. PCR and DNA gel extraction: CDS-tcdA1. Changed pSB1A3 to pSB1C3: plu0840, plu1537, tcdA1. Transformed them into E.coli DH5α, selected from single colonies and used single enzyme digestion to verify the got plasmids.

  • 8.26.2015

    Seamless assembly: plasmid+CDS-tcdA1, plasmid+CDS-plu0840. DNA gel extraction: tcdA1. PCR: device-plu0840.

  • 8.25.2015

    The sequence of ermE+plasmid, Frr+plasmid and plu1537+plasmid were confirmed, which marked the success in standardization of 3 parts!!

  • 8.24.2015

    • Bacteria solution PCR: metK and OrfX. Only negative clone. Extraction of plasmid mCherry and double enzyme digestion. DNA sequencing: ermE+plasmid, Frr+plasmid, plu1537+plasmid. Selected from single colonies of plu1537.

    • Power cut. We played table tennis near our lab.


  • 8.23.2015

    • Selected from single colonies of metK and OrfX.

    • Transformed standard plu1537 plasmid into BL-21. Bacterium solution PCR: ermE and Frr.

    • We kept a Madagascar cockroach as our pet~








  • 8.22.2015

    • Selected from single colonies of ermE and Frr. Extraction of plasmid (standard plu1537).

    • Bacterium solution PCR: standard plu1537.

  • 8.21.2015

    • DNA sequencing. Seamless assembly: plasmid+1537, plasmid+tcdA1-L+ tcdA1-M+ tcdA1-R, plasmid+ermE, plasmid+Frr.

    • Successfully booked the HI-Boston Hostel and paid the full payment~ Seamless assembly: plasmid+metK, plasmid+OrfX.

  • 38th Group meeting

    8.20.2015

    38th Group meeting. Introduced a software named Mou which could enhance the efficiency of writing wiki. Discussed the business planning of our product and the photos on the wiki.

  • 8.20.2015

    • Extraction of plasmid mCherry and double enzyme digestion. Seamless assembly: plasmid+1537, plasmid+tcdB1. PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction.

    • Capsulated Streptomyces Avermitilis toxicity test. TT01 toxicity verification.

    • PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction.

    • Again used flow cytometry to check the diameter of the embedded E.coli and tried to count the E.coli which were successfully embedded. The results were not that ideal again.

  • 8.19.2015

    • PCR: ermE, Frr, metK and OrfX. Clean up and DNA gel extraction.

    • Finished the background of avermectin and toxic protein. Sent bacterium sample to BNU and we would continue to help them.


  • 8.18.2015

    • PCR: CDS tcdB1, CDS 1537, tcdA1-L, mcherry. DNA sequencing.

    • Half-done the model of degration of toxic protein. Got the kit for detection avermectin.

  • 8.17.2015

    • PCR: CDS tcdB1, CDS plu1537 and mCherry. DNA gel extraction: tcdA1-L and plu0840L. Seamless assembly: plasmid+pBad+plu1537.

    • Detected the relationship between distance of food source and food consumption.

    • Designed the primers for Frr, metK, OrfX and ermE again because the need for standardization.

  • 37th Group meeting

    8.16.2015

    37th Group meeting. Built the framework for wiki. Discussed the Syn-bio talks on next Saturday.

  • 8.16.2015

    • Preparation of competent cells.DNA gel extraction.

    • Verification of the bait attractiveness.

  • 8.15.2015

    • PCR: pBad, tcdA1-L, tcdB1, plu0840L and plu1537. Purchased E.coli BL21.

    • The experiment concerning Streptomyces avermitilis finished 80%.

    • Lovely poppy~


  • 8.14.2015

    • PCR and clean up. Concluded the successful PCR parameters of different gene clusters.

    • Test the Avermectin detection kit with pure Ivermectin.

    • Designed the primers for plu0840, plu1537, tcdA1 and tcdB1 again bucause of the need for standardization.


  • 8.13.2015

    • Overlap: pBad and tcdA1-L, pBad and tcd-B1, pBad and plu1537. Extraction of plasmid mCherry and enzyme digestion.

    • Completed wiki page for 2nd model. Drew the 3D ball-and-stick models for CNC with Solidworks. Completed the device for killing termites.

    • Yummy hotpot~

    • Completed the SCM program for HAZU and they affirmed our help.




  • 8.12.2015

    • Overlap: 0840L and 0840R.

    • Tried to purify the embedded E.coli with the E.coli which were able to express RFP.

    • Used flow cytometry to check the diameter of the embedded E.coli and tried to count the E.coli which were successfully embedded. The results were not that ideal.


  • 8.11.2015

    • PCR: pBad, tcdA1-L, tcdA1-M, tcdA1-R, tcdB1, 0840L, 0840R, 1537, GFP.

    • PCR: ligated product. Optimized the PCR program. Cleaned up. PCR again.

    • SEM scanned our samples to verify whether Streptomyces avermitilis were successfully embedded with CNC.

    • Fed the termites with Photorhabdus luminescens TT01 bacterium cells.


  • 8.10.2015

    • PCR: pBad, tcdA1-L and GFP.

    • Used Bgl2 and EcoR1 digested inserted fragment and cleaned up the product. Ligating fragment into plasmid with T4 ligase.

    • Specified the standardization requirements and laid the foundation for primer designing.

    • Mixed blue-stained termites with red-stained termites to verify trophallaxis.


  • 35th Group meeting

    8.9.2015

    35th Group meeting. Planned to hold a seminar for freshmen. Tried to design a device for attracting and killing termites.

  • 8.9.2015

    • PCR: tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R and plu1537.

    • Completed our framework and philosophy of safety.

    • Extracted the plasmid containing RFP from DH5α. PCR: RFP and confirmed by gel electrophoresis.


  • 8.8.2015

    Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).

  • 8.8.2015

    Visited Shanghai Science&Technology Museum and promoted our Synbio-cards(Polypoly card).

  • 8.7.2015

    One step cloning protocol finished. Designed the questionaire about termites.

  • 8.7.2015-8.8.2015

    PCR: pBad, GFP, tcdA1-M, tcdA1-R.

  • 34th Group meeting

    8.6.2015

    34th Group meeting. Communicated with 2 members of SJTU in our lab. Began to write wiki. Attached more importance to safety.

  • 8.6.2015

    • Drawing plasmid circuits in snapgene.(toxic protein)

    • Stained 50 termites in blue and 50 in red for trophallaxis experiments.


  • 8.5.2015

    • Discussed the circuit construction and experiments planning of toxic protein.

    • Decided to give up confocol because we couldn't find the specific dye for CNC.


  • 8.4.2015

    Completed the One Step Cloning protocol.

  • 33rd Group meeting

    8.2.2015

    33rd Group meeting. Began to build framework for wiki. Introduced the habits and characteristics of termites.

  • July

  • 32nd Group meeting

    7.30.2015

    32nd Group meeting. Planned to promote synthetic biology and our project in Shanghai Science & Technology Museum. Polished up the Synbio-cards(Polypoly card).

  • 7.28.2015-8.4.2015

    PCR: pBad, tcdA1-L, tcdA1-M, tcdA1-R, tcdB1, plu0840L, plu0840R, plu1537 and GFP.

  • 7.28.2015

    Giant Jamboree registration completed.

  • 7.26.2015

    • Listed 15 main problems we were confronted with, including safety, communication between dry group and wet groups.

    • Designed a new method to embed the bacterium with CNC.

  • 7.25.2015

    Designed the primers for plu0840, plu1537, tcdA1 and tcdB1 again bucause of the bug of extra RBS.

  • 7.24.2015

    Discussed helping BNU and HUST. Designed and alpha tested the Synbio-cards. Improved the modeling.

  • 7.19.2015-7.24.2015

    Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST.

  • 7.19.2015-7.24.2015

    Attended NCTU meetup in Taiwan. Present our half-done project and communicated with other teams. Decided to help BNU and HUST.

  • 7.18.2015

    • Listed and detailed the wiki requirements.

    • Fed the termites with Streptomyces Avermitilis bacterium cells.

  • 7.16.2015

    Prepared the home page and member page of our wiki. Prepared the poster for NCTU meetup.

  • 7.15.2015

    • Designed the primers for Frr, metK, OrfX and ermE again. Got our name card~

    • Used confocal to verify the successful package of E.coli with CNC. However, the result appeared not ideal and we couldn't find appropriate dye of CNC.


  • 7.14.2015

    Prepared for the souvenirs for NCTU meetup.

  • 31st Group meeting

    7.12.2015

    31st Group meeting. Reviewed the medal requirement and all the rules in lab.

  • 7.11.2015

    Mark-recapture method failed because we failed to recapture enough stained termites.

  • 7.3.2015-7.11.2015

    Exams of summer semester.

  • June

  • 30th Group meeting

    6.25.2015

    30th Group meeting. Planned to verify the package of CNC with confocal. Continued to prepare for the presentation and application scenario.

  • 6.24.2015

    • Completed 2 safety questionaires.

    • Prepared to learn confocol to verify the CNC package for E.coli.

  • 6.22.2015

    Put 150 blue stained termites back into the nest for mark-recapture method.

  • 29th Group meeting

    6.21.2015

    29th Group meeting. Discussed the application scenario for the 5000€ additional sponsership of SYNENERGENE.

  • 6.21.2015

    Contacted with Björn and he would help us animate the process of crystallization.

  • 6.20.2015

    TEM scanned our samples.

  • 6.19.2015

    Fed the termites with Photorhabdus luminescens TT01 bacterium solution to test the natural toxicity. Stained 150 termites in blue for future experiment.

  • 28th Group meeting

    6.18.2015

    28th Group meeting. 2nd modeling half done. Discussed the results of the SEM.

  • 6.17.2015

    TEM and SEM scanned our samples.

  • 6.17.2015

    TEM and SEM scanned our samples.

  • 6.15.2015

    Fed the termites with Streptomyces Avermitilis bacterium solution to test the natural toxicity.

  • 27th Group meeting

    6.14.2015

    27th Group meeting. Rearranged all the events with four quadrant method. Checked everything we have had.

  • 6.14.2015

    Solved the diffusion equation.

  • 6.13.2015

    Extracted the genome of Streptomyces avermitilis.

  • 6.12.2015

    Went to shanghai for the interview of visa. 12 members successfully passed the interview.

  • 26th Group meeting

    6.11.2015

    26th Group meeting. Discussed the half-done presentation and Skype conference with SYNENERGENE. Prepared for the visa.

  • 6.11.2015

    PCR: plu1537, with the template of the genome of TT01.

  • 6.10.2015

    • Paid for the air tickets.

    • Got the primers of toxic protein.

  • 6.8.2015

    Optimization of the model of termites finding food.

  • 25th Group meeting

    6.7.2015

    25th Group meeting. Polished up the previous modeling. Protocols about the experiments concerning bacterium. A member quit.

  • 6.7.2015

    Extracted the genome of TT01.

  • 6.6.2015

    Frozen the spores of Streptomyces avermitilis.

  • 24th Group meeting

    6.4.2015

    24th Group meeting. Brainstormed the Synbio-cards and Synbio-workshops. Listed the datailed personal scheme about the time arrangement before summer holiday.

  • 6.3.2015

    • Discussed the characterization of E.coli embedded in CNC, including TEM, SEM, confocal, FTIR and flow cytometry.

    • Our proposal had been accepted and our team would be awarded financial support from SYNENERGENE! Cheers~

    • Brainstormed how to do human practice and use the €5000. We came up with the Synbio-cards and Synbio-workshops.

  • 6.2.2015

    Began to book the air tickets. Booked the first primers of toxic protein for one step cloning. Found the sequence of the gene we need in the Streptomyces avermitilis.

  • 6.1.2015

    SEM scanned our samples again. Debugged the first modeling.

  • May

  • 23rd Group meeting

    5.31.2015

    23rd Group meeting. Discussed the half-done modeling.

  • 5.31.2015

    Built up the model of termites finding food.

  • 5.31.2015

    Taught high school students to do experiment to extract DNA from epithelial cell of mouth.




  • 5.29.2015

    • Repeated the cellulase activity detection experiment. Meanwhile we measured the lysozyme activity in different parts of termites guts.

    • 22nd Group meetig. Specified the medal requirement. Began to prepare for the NCTU meetup.

    • SEM scanned our samples.

  • 5.28.2015

    Completed a model of a cartoon termite holding a sword. Cleaned up our lab and rearranged all the experiment materials we would need.

  • 5.26.2015

    Got the solid CNC with lyophilization. Built the framework of the first web page.

  • 5.25.2015

    Learned the skills of culturing Streptomyces avermitilis in the professor's lab.

  • 21st Group meeting

    5.24.2015

    21st Group meeting. Began to prepare for the presentation.

  • 5.22.2015

    iGEM Shanghai Tour. Took part in the conference held in NYU. Visited FDU and communicated with them.

  • 5.21.2015

    Chosen the certain toxic protein which we would transfer into E.coli: tcdA1, tcdB1,plu0840 and plu1537.

  • 5.21.2015

    20th Group meeting. Discussed the medal requirements and collaboration among groups.

  • 5.20.2015

    • Finished the protocol about TT01.

    • Measured the cellulase activity in termites head, forgut, midgut and hindgut.

  • 5.19.2015

    Detailed the medal requirements depending on our project. Finished the protocol about Streptomyces avermitilis.



  • 5.18.2015

    Got the E.coli strain ET12567 from Professor Li's Lab. Began the experiment about Streptomyces avermitilis.

  • 18th Group meeting

    5.17.2015

    18th Group meeting. Emphasized the lab safety.

  • 5.17.2015

    Did experiments(rainbow kit)related to synthetic biology in Zhejiang Science&Technology Museum with high school students and by this way promoted syn-bio.

  • 5.15.2015

    Read paper and discussed how to enhance the efficiency of Avermectin in Streptomyces avermitilis.

  • 17th Group meeting

    5.14.2015

    17th Group meeting. Discussed the results of the TEM. Tried to contact with a judge from PKU.

  • 5.14.2015

    • TEM scanned our samples: using CNC to pack the Bacillus subtilis.

    • Completed the cover and comic of our proposal which would be handed in to SYNENERGENE.

    • Contacted with Hangzhou Termites control Institute.


  • 5.14.2015

    • TEM scanned our samples: using CNC to pack the Bacillus subtilis.

    • Completed the cover and comic of our proposal which would be handed in to SYNENERGENE.

    • Contacted with Hangzhou Termites control Institute.


  • 5.13.2015

    • Got the genome of tcdA1 and tcdB1 in NCBI.

    • Got TT01(a species of Photorhabdus luminescens) which could produce toxic protein. It would play an important role in our plan B.

  • 5.12.2015

    Successfully checked in termites and tc protein family.

  • 16th Group meeting

    5.10.2015

    16th Group meeting. Communication between dry group and 3 wet groups. Arranged time for experiments.

  • 15th Group meeting

    5.7.2015

    15th Group meeting. Further discussed the proposal and allocated the tasks. Continued to consult professors about several species of bacterium.

  • 5.6.2015

    Gave up synthesizing avermectin in Bacillus subtilis. Tried to overexpress avermectin in E.coli and then transformed its plasmid into Streptomyces avermitilis.

  • 5.6.2015

    Successfully got the solution of CNC!! And it appeared Tyndall effect.

  • 5.4.2015-5.6.2015

    Exams of spring semester.

  • 5.3.2015

    Our termite nest was sent out from Guangdong Province.

  • April

  • 14th Group meeting

    4.29.2015

    14th Group meeting. 10 members would go to Taiwan for NCTU meetup. Results of consulting professors specialized in termites and Bacillus subtilis.

  • 4.28.2015

    • Discussed the plan for embedding baterium with CNC and the self-assembly of CMC and chitosan on the surface of baterium.

    • Debugged the problem of synthesizing avermectin in Streptomyces avermitilis. Consulted professors specialized in synthesizing avermectin in Streptomyces avermitilis.

  • 4.27.2015

    Consulted professors specialized in termites and Bacillus subtilis.

  • 13th Group meeting

    4.26.2015

    13th Group meeting. Discussed something important: the proposal for the 5000€ additional sponsership of SYNENERGENE and visa. A new mamber joined our team. Specified the problem remaining to be solved of each group.

  • 4.25.2015

    Discussd the advantage of Bacillus subtilis campared with E.coli and the utilization of spore of Bacillus subtilis.

  • 12th Group meeting

    4.23.2015

    12th Group meeting. Debugged the previous package plan and decided to pack bacterium with CNC. Planned to buy a nest of termites from Guangdong Province. Discussed several demensions of modeling in this project.

  • 4.22.2015

    Consulted professors specialized in termites.

  • 4.21.2015

    Consulted professors about directly embedding baterium with CNC rather than with the aid of protein domain. Our idea was confirmed and praised.

  • 4.20.2015

    Communication with our instructor. Searched for information about cellulose binding domain(CBDs) and overexpression of ivermectin and avermectin.

  • 11th Group meeting

    4.19.2015

    11th Group meeting. Decided our project: termite terminator. Allocated all members into 4 groups. Each group was responsible for a part of our project.

  • 10th Group meeting

    4.16.2015

    10th Group meeting. Decided to participate in NCTU meetup in summer holiday and apply for the 5000€ additional sponsership of SYNENERGENE. Discussed 3 existing ideas.

  • 4.14.2015

    Preliminarily designed the circuit in Streptomyces avermitilis.

  • 9th Group meeting

    4.12.2015

    9th Group meeting. Kept 3 ideas and the final idea would derive from them. Detailed these 3 ideas from all the aspects.

  • 8th Group meeting

    4.9.2015

    8th Group meeting. Continued to kill unappropriate ideas. Recalled the aim of participating iGEM and reached a consensus.

  • 7th Group meeting

    4.6.2015

    7th Group meeting. Began to kill some undoable ideas and further dug the existing ideas, such as biological enigma machine and killing termites.

  • 6th Group meeting

    4.2.2015

    6th Group meeting. Developed several new ideas, including bio-SCM, biological enigma machine and slow release of certain chemical. Talked about their feasibility.

  • March

  • 5th Group meeting

    3.29.2015

    5th Group meeting. Dug previous ideas and considered their safety and feasibility.

  • 4th Group meeting

    3.26.2015

    4th Group meeting. Continued to share and improve our ideas, such as bio-lens, detection of schistosomiasis and killing termites.

  • 3.25.2015

    Team application completed.

  • 3rd Group meeting

    3.22.2015

    3rd Group meeting. Discussed some ideas about modeling. Shared new ideas.

  • 3.21.2015

    Communicate with Zhejiang Science&Technology Museum.

  • 2nd Group meeting

    3.19.2015

    2nd Group meeting. Built up several rules and principles. Tried to make the most of our BBS.

  • 1st Group meeting

    3.16.2015

    1st Group meeting. Everyone was assigned with his/her own mission. Brainstorm began.

  • 3.15.2015

    Visited the AST space of Zhejiang Science&Technology Museum. Decided to collaborate with.

  • 3.13.2015

    Concluded the winter project.

  • Winter

  • 2.1.2015-3.13.2015

    Everyone read wikis, learned something from previous projects and attempted to come up with new ideas.

  • 1.31.2015

    1st meetup. Had a nice meal~

  • 1.30.2015

    15 members was picked out. ZJU-China 2015 team was set up~ Our story began.


termit