Difference between revisions of "Team:HKUST-Rice/Design"

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<h2>Design</h2>
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<link rel="stylesheet" href="https://2015.igem.org/Template:HKUST-Rice/CSS?action=raw&ctype=text/css" type="text/css" />
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<style type= "text/css">
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<h1>project title </h1>
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<h4>Note</h4>
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Applied Design award</a> and/or the <a href="https://2015.igem.org/Judging/Awards#Medals">functional prototype gold medal criterion</a>, you must fill out this page.</p>
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margin: 50px auto;
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font-size: 140%;
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<p>This is a prize for the team that has developed a synthetic biology product to solve a real world problem in the most elegant way. The students will have considered how well the product addresses the problem versus other potential solutions, how the product integrates or disrupts other products and processes, and how its lifecycle can more broadly impact our lives and environments in positive and negative ways.</p>
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<p>
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table.catalog_table caption {
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If you are working on art and design as your main project, please join the art and design track. If you are integrating art and design into the core of your main project, please apply for the award by completing this page.
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<br>
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<div class= "project_superrow">
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<div id= "page_title"><h1>DIY Gel Imaging Station</i></h1></div>
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<div id="MYicon1">
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<a href="https://2015.igem.org/Team:HKUST-Rice/Expression"><img src="https://static.igem.org/mediawiki/2015/e/ea/HKUST-Rice15_leftarrow.png">
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<p style="color:#5570b0; font-size: 130%"> Application </p></a>
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</div>
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<div class="project_content">
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<div class="project_row">
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<h1>Why Gel imaging is important to synthetic biology? </h1>
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<p>Gel electrophoresis is one of the most essential tools in biology, it is an essential part for restriction diagnostic test for nucleic acid, resolving PCR products, etc. Having a gel imaging tool in proximity can increase the productivity of the lab. In our case, since the closest gel imaging tools is far away from HKUST iGEM lab, we decided to build a gel imaging station for our own use, using simple and easy-to-get materials. </p>
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</div>
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<div class="project_row">
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<h1>Material for  DIY Gel imaging Station</h1>
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<p>The DIY Gel imaging station mainly consist of five parts:</p>
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<div class="project_image">
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<img src="https://static.igem.org/mediawiki/2015/thumb/c/c4/HKUST_Rice15_still_whole_gel_doc_web_annotated.png/800px-HKUST_Rice15_still_whole_gel_doc_web_annotated.png" alt="Parts of DIY Gel Imaging station " width= "800px">
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</div>  
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<div class="project_image">
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<img src="https://static.igem.org/mediawiki/2015/thumb/0/0f/HKUST_Rice_Green_Filter_annotated.png/800px-HKUST_Rice_Green_Filter_annotated.png" alt="Parts of DIY Gel Imaging station " width= "800px">
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</div>
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<div class="project_image">
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<img src="https://static.igem.org/mediawiki/2015/thumb/f/f4/HKUST_rice15_UV_trans_illunminator_annotated.png/800px-HKUST_rice15_UV_trans_illunminator_annotated.png" alt="Parts of DIY Gel Imaging station " width= "800px">
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</div>  
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<table class= "catalog_table">
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<tr><td>Item</td><td>Source</td><td>Cost (HKD)</td></tr>
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<tr><td>1: Case </td><td>Local hard ware store: Pricerite</td><td>~$100</td></tr>
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<tr><td>2: Camera</td><td>Local electronic store</td><td>~$3000</td></tr>
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<tr><td>3: Computer with monitor for live viewing of Gel</td><td>Laboratory Laptop</td><td>~$5000</td></tr>
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<tr><td>4: Color filter</td><td>TaoBao</td><td>~$30</td></tr>
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<tr><td>5: UV trans-illuminator</td><td>HKUST division of life science</td><td></td></tr>
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<tr><td>Total</td><td></td><td></td></tr>
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</table>
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<p> In addition to the materials listed above, a stand and clamp is used to hold the camera in place. A heat block is used as a counter weight to keep the set up balance. The inside of the case is wrapped with two layers of aluminium foil to insulate the UV radiation emitted from the UV trans-illuminator when it is being turned on. </p>
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<p> A small hole is drilled at the back of the plastic case (not shown on picture above). This hole allows connection between the power cord and the UV trans-illuminator. </p>>
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</div>
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<div class="project_row">
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<h1>From Prototype to a Working Model</h1>
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<h2>Prototype</h2>
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<p>Before we built the DIY Gel imaging station , we built a prototype to prove the feasibility of a DIY gel imaging station . Here is how we build this prototype:</p>
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<div class="project_image">
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<img src="https://static.igem.org/mediawiki/2015/thumb/d/d3/HKUST_Rice15_gel_doc_prototype1_web.png/337px-HKUST_Rice15_gel_doc_prototype1_web.png" alt="Parts of DIY Gel Imaging station ">
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</div>
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<ol>
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<li>We obtained a plastic bucket and drilled a 3-inch hole on the bottom of it.  </li>
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<li>We placed a filter on top of the hole and put a camera on top of the filter. </li>
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<li>An electrophoresed agarose gel stained with Midori Green is placed on top of the UV trans-illuminator</li>
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<li>The entire bucket, with the filter and camera are placed on top of the UV trans-illuminator, and a photo was taken </li>
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</ol>
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<h2>Working Model</h2>
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<p>After we confirmed that we could take Gel photo using easy-to-get materials, we proceeded to build a case especially for this purpose. With the case, the imaging station will be more durable. We choose plastic as the material for case. Plastic is resistant to rust and inert to acid and base. Using plastic can <b>prevent rusting</b> and is <b>easy to maintain</b>.</p>
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<div class="project_image">
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<img src="https://static.igem.org/mediawiki/2015/thumb/a/a9/HKUST_Rice15_still_image_diy_gel_doc_web.png/800px-HKUST_Rice15_still_image_diy_gel_doc_web.png" alt="First image taken by the DIY gel imaging station">
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</div>
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<p>A photo was taken using this model of DIY Gel imaging station to prove the functionality of the station. This is the 1<sup>st</sup> photo taken:</p>
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<div class="project_image">
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<img src="https://static.igem.org/mediawiki/2015/9/94/HKUST_Rice15_diy_gel_imaging_Gel_web.png">
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</div>
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<p>The above gel is 0.8% agarose gel pre-stained with Midori Green Gel Advance DNA stain. Each lane is loaded with NEB 1 kb DNA Ladder(N3232, NEB). A green filter is used in taking the photo. As shown on the picture above, each band is clearly shown. Each band is bright enough to be observed. </p>
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{{HKUST-Rice Directory}}

Revision as of 21:22, 15 September 2015


DIY Gel Imaging Station

Why Gel imaging is important to synthetic biology?

Gel electrophoresis is one of the most essential tools in biology, it is an essential part for restriction diagnostic test for nucleic acid, resolving PCR products, etc. Having a gel imaging tool in proximity can increase the productivity of the lab. In our case, since the closest gel imaging tools is far away from HKUST iGEM lab, we decided to build a gel imaging station for our own use, using simple and easy-to-get materials.

Material for DIY Gel imaging Station

The DIY Gel imaging station mainly consist of five parts:

Parts of DIY Gel Imaging station
Parts of DIY Gel Imaging station
Parts of DIY Gel Imaging station
ItemSourceCost (HKD)
1: Case Local hard ware store: Pricerite~$100
2: CameraLocal electronic store~$3000
3: Computer with monitor for live viewing of GelLaboratory Laptop~$5000
4: Color filterTaoBao~$30
5: UV trans-illuminatorHKUST division of life science
Total

In addition to the materials listed above, a stand and clamp is used to hold the camera in place. A heat block is used as a counter weight to keep the set up balance. The inside of the case is wrapped with two layers of aluminium foil to insulate the UV radiation emitted from the UV trans-illuminator when it is being turned on.

A small hole is drilled at the back of the plastic case (not shown on picture above). This hole allows connection between the power cord and the UV trans-illuminator.

>

From Prototype to a Working Model

Prototype

Before we built the DIY Gel imaging station , we built a prototype to prove the feasibility of a DIY gel imaging station . Here is how we build this prototype:

Parts of DIY Gel Imaging station
  1. We obtained a plastic bucket and drilled a 3-inch hole on the bottom of it.
  2. We placed a filter on top of the hole and put a camera on top of the filter.
  3. An electrophoresed agarose gel stained with Midori Green is placed on top of the UV trans-illuminator
  4. The entire bucket, with the filter and camera are placed on top of the UV trans-illuminator, and a photo was taken

Working Model

After we confirmed that we could take Gel photo using easy-to-get materials, we proceeded to build a case especially for this purpose. With the case, the imaging station will be more durable. We choose plastic as the material for case. Plastic is resistant to rust and inert to acid and base. Using plastic can prevent rusting and is easy to maintain.

First image taken by the DIY gel imaging station

A photo was taken using this model of DIY Gel imaging station to prove the functionality of the station. This is the 1st photo taken:

The above gel is 0.8% agarose gel pre-stained with Midori Green Gel Advance DNA stain. Each lane is loaded with NEB 1 kb DNA Ladder(N3232, NEB). A green filter is used in taking the photo. As shown on the picture above, each band is clearly shown. Each band is bright enough to be observed.