Difference between revisions of "Team:Georgia State/Collaborations"

Our interest in our topic began when we were researching possible projects. What we found most interesting was a research paper from the Journal of Pakistan Medical Association that tested the efficacy of microwaving LB rather than autoclaving. Also, a project that inspired us was the project from the University of Calgary that tested the efficacy of a homemade wash for competent cells. From these projects, we asked ourselves: Could one conduct a synthetic biology project without access to a professional laboratory?

This summer, we created a cost effective alternative to lab grade Luria Broth (LB). To compare our homemade broth, J-Broth, and lab grade LB we compared optical densities of liquid cultures and transformation efficiency of competent cells. For each method of testing, we compared autoclaved LB, microwaved LB, and microwaved J-Broth.

These are the materials we used. The cost almost nothing! You can even buy them off of Amazon!