Difference between revisions of "Team:NAIT Edmonton/Protocols"

Line 760: Line 760:
 
         2. Mix the following components<br>
 
         2. Mix the following components<br>
 
<center>
 
<center>
<table >
+
<table class="tg">
 
<tr>
 
<tr>
 
<strong>
 
<strong>
<td style="border:1px solid black;">Component</td>
+
<th class="tg-hgcj">Component</th>
<td style="border:1px solid black;">Amount</td>
+
<th class="tg-hgcj">Amount</th>
 
</strong>
 
</strong>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>Enzyme 1</td>
+
<td class="tg-s6z2">Enzyme 1</td>
<td>1µL</td>
+
<td class="tg-s6z2">1µL</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>Enzyme 2</td>
+
<td class="tg-s6z2">Enzyme 2</td>
<td>1µL</td>
+
<td class="tg-s6z2">1µL</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>DNA</td>
+
<td class="tg-s6z2">DNA</td>
<td>1µg</td>
+
<td class="tg-s6z2">1µg</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>Buffer</td>
+
<td class="tg-s6z2">Buffer</td>
<td>5µL</td>
+
<td class="tg-s6z2">5µL</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>Nuclease Free H<sub>2</sub>O</td>
+
<td class="tg-s6z2">Nuclease Free H<sub>2</sub>O</td>
<td>Up to 50µL</td>
+
<td class="tg-s6z2">Up to 50µL</td>
 
</tr>
 
</tr>
<caption><f6>
+
 
*Ratio of enzymes to DNA is 1:1 and water is added to maintain the concentration based on each enzyme</f6>
+
</caption>
+
 
</table>
 
</table>
  
</center>
+
<*Ratio of enzymes to DNA is 1:1 and water is added to maintain the concentration based on each enzyme/center>
 
<br><br>
 
<br><br>
 
3. Let reaction occur for 1 hour
 
3. Let reaction occur for 1 hour

Revision as of 01:37, 16 September 2015

Team NAIT 2015

Experimental Design

Go through our interactive experimental design flow chart! Many of our protocols are manufacturer specicfied; however, some are customized by us! Click on the flow chart boxes if the hand cursor appears to read more about our customized protocols.

PDFs of protocols can also be found

Theorizing our Sequences
Literature has shown certain proteins inherently stain in colour.
Looked up characteristics of said proteins
Isolated and identified the unique characteristics of said proteins so that we can manually write our own sequences and generate custom proteins.
Writing our Sequences
Went down to base pair level and wrote out our sequences with the defining characteristics identified in literature.
PCR
Digestion and Ligation
Transforming Bacteria
Validating the Transformation
Protein Isolation and Purification
SDS PAGE and Silver Staining