Difference between revisions of "Team:Georgia State/Collaborations"
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− | <h2 class="h3" style="text-align: left;color: black;">Project JBroth</h2> | + | <h2 class="h3" style="text-align: left;color: black;">Backyard Biology @ Emory University: Project JBroth</h2> |
<h3 class="h4" style="text-align: left;color: black;">Low Cost LB and Competent Cells!</h3> | <h3 class="h4" style="text-align: left;color: black;">Low Cost LB and Competent Cells!</h3> | ||
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− | <p class="lead" style="color: rgb(0, 0, 0);"> Our interest in our topic began when we were researching possible projects. What we found most interesting was a research paper from the Journal of Pakistan Medical Association that tested the efficacy of microwaving LB rather than autoclaving. Also, a project that inspired us was the project from the University of Calgary that tested the efficacy of a homemade wash for competent cells. From these projects, we asked ourselves: Could one conduct a synthetic biology project without access to a professional laboratory? </p> | + | <p class="lead" style="color: rgb(0, 0, 0);"> Our interest in our topic began when we were researching possible projects. What we found most interesting was a research paper from the Journal of Pakistan Medical Association that tested the efficacy of microwaving LB rather than autoclaving. Also, a project that inspired us was the project from the University of Calgary (2014) that tested the efficacy of a homemade wash for competent cells. From these projects, we asked ourselves: Could one conduct a synthetic biology project without access to a professional laboratory? </p> |
<p class="lead" style="color: rgb(0, 0, 0);"> This summer, we created a cost effective alternative to lab grade Luria Broth (LB). To compare our homemade broth,<b> J-Broth</b>, and lab grade LB we compared optical densities of liquid cultures and transformation efficiency of competent cells. For each method of testing, we compared autoclaved LB, microwaved LB, and microwaved J-Broth. </p> | <p class="lead" style="color: rgb(0, 0, 0);"> This summer, we created a cost effective alternative to lab grade Luria Broth (LB). To compare our homemade broth,<b> J-Broth</b>, and lab grade LB we compared optical densities of liquid cultures and transformation efficiency of competent cells. For each method of testing, we compared autoclaved LB, microwaved LB, and microwaved J-Broth. </p> |
Revision as of 02:20, 16 September 2015
Collaborations
Backyard Biology @ Emory University: Project JBroth
Low Cost LB and Competent Cells!
![](https://static.igem.org/mediawiki/2015/6/6d/LowBudgetSticker_GSUiGEM.png)
Our interest in our topic began when we were researching possible projects. What we found most interesting was a research paper from the Journal of Pakistan Medical Association that tested the efficacy of microwaving LB rather than autoclaving. Also, a project that inspired us was the project from the University of Calgary (2014) that tested the efficacy of a homemade wash for competent cells. From these projects, we asked ourselves: Could one conduct a synthetic biology project without access to a professional laboratory?
This summer, we created a cost effective alternative to lab grade Luria Broth (LB). To compare our homemade broth, J-Broth, and lab grade LB we compared optical densities of liquid cultures and transformation efficiency of competent cells. For each method of testing, we compared autoclaved LB, microwaved LB, and microwaved J-Broth.