Difference between revisions of "Team:NAIT Edmonton/Practices"

We often find that even though we try to account for every potential problem, there is always one that should have been obvious. This scenario is inevitable and more often than not, will happen at the worst time possible. How we handle these unforeseen problems can result in either a successful mission or a critical mission failure. As a team competing for the first time, there was no shortage of these scenarios and as a good team should, we came together and quickly found solutions for the problems. As the summer passed, our knowledge and experience grew and we decided to reach out to the teams in our track to compile all of our collective experiences and perspectives on the iGEM experience and put it together to create a tool for future team’s use.

In this guide you will find multiple team’s perspective on topics such as design, laboratory work, funding, team building and the suggested approach to potential problems that can emerge. However, our vision for this guide is to not only provide insight and solutions to some of these unforeseen problems but to also challenge the way in which we perform established practices used in the preparation for the iGEM competition. In future we hope to evolve this guide into a forum where all iGEM teams can contribute to each other’s knowledge, because we believe the main goal of the iGEM competition, at its core, is to provide students with an opportunity to learn and grow the scientific community.

So you’ve heard about this competition called iGEM and you are raring to go! But before you hit the labs, you need an idea for a project, so how do you actually get an idea? Through interviewing some of the teams we found that there was a few methods you can use to come up with an idea to pursue for the competition.

Let’s take a look at some of these Teams:

The University of KU Leuven is a long time participant in the iGEM competition, having competed for the last five years. Every year, however, there is a new group of students, and this year their team chose not to build on the previous year’s work, but to create their own.

So how did they design their project?

Team KU Leuven’s approach was, starting from the month of January to do weekly meetings to discuss potential project ideas in small groups and then narrow the ideas down so that the team collectively decide on one topic for the focus of their research. Once the project was decided on, when the summer started they were able to focus on their lab work to bring their vision to life.

We believe that this was a successful method to approach the project design, because of their time management. By starting in January, they gave themselves the freedom to really explore what problem they can address using synthetic biology and to properly plan what resources they would need for the summer.

However it is important to note that this was done during their winter semester and while participating in the iGEM competition is an extremely good learning opportunity and experience, we must remember that we should not let it distract us while in the classroom. Interestingly enough, some universities actually have courses that can prepare you for the competition! For example at the University of Alberta, through the department of biochemistry in the Faculty of Medicine and Dentistry they offer BIOCH 482: Design and Construction of Synthetic Biological Systems. This course is designed to prepare students for participation in the iGEM Competition through team-based problem solving. (http://biochem.med.ualberta.ca/Education/Undergraduate/Pages/Courses.aspx#sthash.Ba3gtYMv.dpf)

So if you choose to use Team KU Leuven’s approach to project design, be sure to check your course listings at your institution to see if you can make the make the experience work doubly for you!

We’ve taken a look at a team that designed before the summer now let’s take a look at a team that designed at the beginning of summer…

The Eindhoven Institute of Technology is no stranger to iGEM. Their institution has competed multiple times, and they have a new team each year with a unique project. So how did they choose to design their project for this year’s competition circuit?

In the first month they began searching for problems and possible solutions as using the previous Eindhoven teams’ work as a guide as well. Through a series of presentations and small group discussions they realized that some of their proposed solutions to those problems were not possible due to lack of information, given the “new-ness” of the problems.

So what did they do?

They used the 2014 Eindhoven project for inspiration; inspired by Eindhoven 2014’s “Click Coli” project they used the previous year’s work as the foundation for a new type of Modular Biosensor! By making this decision early in the summer, they are able to not only come up with a feasible project idea, but also not cut into the bulk of summer time by overly focusing on a project idea.

Some can argue that designing a project at the start of the summer is not a practical idea, however we have seen that it is possible to still come up with a good project design by analysing and building on previous years’ work. At the jamboree we see many new and exciting projects, and while it is important to constantly explore new ideas, we must not forget that we can always improve upon previous years’ work. Whether it is improving the characteristic of a previous bio-brick or using a previous team’s project for inspiration, any improvement is a step forward.

We’ve seen two different approaches to project design from different teams, so how did we ,Team NAIT_Edmonton, design our project?

This year will our first time competing in the iGEM competition and despite looking at previous teams’ work we were having difficulty choosing a project. We started this planning in late spring and with summer quickly approaching, what would we do?

We decided to rely on our mentor Dr. Marcelo Marcet for inspiration for a project. He provided us with a few ideas, and after reviewing them we decided on one that was both practical and had potential for future applications.

In an interview we had with Dr. Marcet, he had this advice to give; “For a good project design, you need two things, knowledge and experience. Knowledge comes from our education and experience comes from actually experiencing the challenges. By combining these two together we can not only come up with a solution to the problem but also appreciate it.”

As first time iGEM’s this advice resonated with us. By learning from him and gaining experience by practicing synthetic biology, we can apply this thinking to not only the iGEM competition but also to any organisation we are a part of.

The communication of our ideas and project before the Jamboree is mostly done on our Wikis. Many of the judges, prior to attending a presentation session, will check the team’s Wiki to ready themselves for the technical talk and to gain a first look and a basic understanding of the project. Additionally, as it is accessible by all, the Wiki can be used as a platform to introduce projects and synthetic biology to the general public. How the Wiki is designed and how it conveys information is therefore extremely important not only for the iGEM competition, but also for how your project is perceived in society’s eye. “When you do things right, people won’t be sure you’ve done anything at all.” When people visit your Wiki, you want them to read and enjoy the content you’ve provided. People can inherently tell if there is something off about your design. If your wiki delivers all the necessities and no one can point out a flaw, you’ve done your job. Designing a functional Wiki was one of the hardest things our team had to tackle. In addition to the complexity of HTML, Java, and CSS (to say the least) we also had to make sure our content was readable and fun to learn. Essentially, your Wiki is an extension of your presentation. Your Wiki is the first impression that you are going to give the judges and anyone else who happens to stumble upon the iGEM site. Your Wiki is important. It is not something you should leave to the last minute. Start on it early and make sure you back up everything you do!

Many of the design principles used to design your wiki can also be applied to your poster and presentation. In fact, having some sort of recurring theme and unity between your wiki, presentation and poster would be an excellent idea. For example, using the same colour scheme and logos would connect all of your platforms together.

1. Content. Content. Content.
2. Make finding information easy.
3. Make learning your project fun.
4. Organize content logically.
5. Less is more. Simple is the way to go.

Our vision for this guide, is for it to not only be used as a reference by future teams, but to eventually transform into a forum where teams can openly collaborate with each other on brainstorming solutions for problems that occur during the research process. We were inspired by the 2015 Paris Bettencourt team’s iGEM Rhizi website and we reached out to them for advice on how they approached its creation, and they responded! They advised on using resources available at your institution and to reach out to upperclassmen/alumni to bring the idea to life. While we are a long ways off from seeing our idea evolve into a website like iGEM Rhizi, we plan to keep adding to the guide and eventually develop it into an open source website for use in future iGEM competitions.

We were asked by one of the members of our institution, “What will you do when you land back in Edmonton after iGEM?” Go to class, obviously. But beyond that, we will look around us and realize how truly lucky we are to have experienced something like the International Genetically Engineered Machine competition. Yes, it was a long, arduous road. Yes, sometimes we felt like quitting but… we didn’t. You didn’t.

So although there may be trials and tribulations, tears and frustration, anger and fear, there is one thing that you must remember - you did it. Whether you get the results you desire or not you have already won. Although they may not be the results that you expected or wanted, you learned something from them. You fostered new friendships and met great mentors. You polished your lab techniques and skills. Most importantly, you received something many students do not get – an authentic learning experience.

We were lucky to have our mentor, Dr. Marcelo Marcet, constantly remind us that iGEM is not about the results or the medals – but about learning. And if there is one thing that we want you to take away from our handbook is that iGEM is about learning. Always keep learning.

Best of luck - Team NAIT 2015