Difference between revisions of "Team:NCTU Formosa/Safe Project Design"
| Line 191: | Line 191: | ||
<tr><td>pSB1C3</td><td>Plasmid backbone</td><td>Kit</td><td>http://parts.igem.org/Part:pSB1C3</td></tr> | <tr><td>pSB1C3</td><td>Plasmid backbone</td><td>Kit</td><td>http://parts.igem.org/Part:pSB1C3</td></tr> | ||
<tr><td colspan="4">Expression Backbone</td></tr> | <tr><td colspan="4">Expression Backbone</td></tr> | ||
| + | <tr><td>pSB1C3</td><td>Plasmid backbone</td><td>Kit</td><td>http://parts.igem.org/Part:pSB6A1</td></tr> | ||
</table> | </table> | ||
| + | |||
| + | <h2>Inserts:</h2> | ||
| + | <table><tr><td width=30%>Name</td><td width=10%>Natural Function</td><td width=10%>Acquired By</td><td width=40%>Notes</td></tr> | ||
| + | <tr><td colspan="4">Promoters</td></tr> | ||
| + | <tr><td>J23101</td><td>Consitutive promoter</td><td>Kit</td><td>http://parts.igem.org/Part:BBa_J23101</td></tr> | ||
| + | <tr><td>J23110</td><td>Consitutive promoter</td><td>Kit</td><td>http://parts.igem.org/Part:BBa_J23110</td></tr> | ||
| + | <tr><td>R0010</td><td>Induced promoter</td><td>Kit</td><td>http://parts.igem.org/Part:BBa_R0010</td></tr> | ||
| + | <tr><td colspan="4">Ribosome Binding Site (RBS)</td></tr> | ||
| + | <tr><td>B0034</td><td>Strong; efficiency 1.0</td><td>Kit</td><td>http://parts.igem.org/Part:BBa_B0034</td></tr> | ||
| + | <tr><td>B0030</td><td>Weak; efficiency 0.3</td><td>Kit</td><td>http://parts.igem.org/Part:BBa_B0032</td></tr> | ||
</div> | </div> | ||
Revision as of 07:14, 16 September 2015
Safe Project Design
Choosing a non-pathogenic chassis
Bacterial strain: Escherichia coli K12
Escherichia coli K-12 are not a threat to human, animal (chickens, pigs, and calves), plants or the environment, according to the 1997 Final Risk Assessment of Environmental Protection Agency (EPA). “Any concerns in terms of health considerations are mitigated by its poor ability to disseminate, colonize the colon and establish infections in a murine model” (Smith et al., 2010). In addition, the chance of the insertion gene mutating the bacterial strain to be hazardous is minimal as the inserting genetic materials have to meet the EPA’s criteria (limited in size, well characterized, free of certain nucleotide sequences, and poor mobility).
Choosing parts that will not harm humans/ animals/ plants
| Name | Natural Function | Acquired By | Notes |
| Cell lines | |||
| H292 (Mucoepidermoid Pulmonary Carcinoma cell) | Cell | Company | |
| HCC827 (Adenocarcinoma) | Cell | Company | |
| H1975 (Adenocarcinoma) | Cell | Company | |
| MCF7 (Adenocarcinoma) | Cell | Company | |
| MDA-MB-231(Adenocarcinoma) | Cell | Company | |
| SK-BR-3 (Adenocarcinoma) | Cell | Company | |
| Normal Backbone | |||
| pSB1A3 | Plasmid backbone | Kit | http://parts.igem.org/Part:pSB1A3 |
| pSB1K3 | Plasmid backbone | Kit | http://parts.igem.org/Part:pSB1K3 |
| pSB1C3 | Plasmid backbone | Kit | http://parts.igem.org/Part:pSB1C3 |
| Expression Backbone | |||
| pSB1C3 | Plasmid backbone | Kit | http://parts.igem.org/Part:pSB6A1 |
Inserts:
| Name | Natural Function | Acquired By | Notes |
| Promoters | |||
| J23101 | Consitutive promoter | Kit | http://parts.igem.org/Part:BBa_J23101 |
| J23110 | Consitutive promoter | Kit | http://parts.igem.org/Part:BBa_J23110 |
| R0010 | Induced promoter | Kit | http://parts.igem.org/Part:BBa_R0010 |
| Ribosome Binding Site (RBS) | |||
| B0034 | Strong; efficiency 1.0 | Kit | http://parts.igem.org/Part:BBa_B0034 |
| B0030 | Weak; efficiency 0.3 | Kit | http://parts.igem.org/Part:BBa_B0032 |
