Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w9"
(→pdark natural transformation) |
(→pcpcG2 electro-transformation) |
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====pcpcG2 electro-transformation==== | ====pcpcG2 electro-transformation==== | ||
| − | + | ||
| + | =====In the evening of Sep. 2===== | ||
2nd liquid segregation of pcpcG2-HR | 2nd liquid segregation of pcpcG2-HR | ||
| − | + | =====In the evening of Sep. 4===== | |
3rd liquid segregation of pcpcG2-HR | 3rd liquid segregation of pcpcG2-HR | ||
| − | + | =====In the evening of Sep. 6===== | |
4th liquid segregation of pcpcG2-HR | 4th liquid segregation of pcpcG2-HR | ||
Revision as of 08:49, 16 September 2015
Contents
Transformation
pdark natural transformation
In the evening of Aug 31
3rd liquid segregation of pdark-HR
In the evening of Sep 3
4th liquid segregation of pdark-HR
In the evening of Sep 6
5th liquid segregation of pdark-HR
pcpcG2 electro-transformation
In the evening of Sep. 2
2nd liquid segregation of pcpcG2-HR
In the evening of Sep. 4
3rd liquid segregation of pcpcG2-HR
In the evening of Sep. 6
4th liquid segregation of pcpcG2-HR
second desalination test of pdark-HR
on 2nd, Sep
inoculated 24 wildtype & 24 pdark-HR (use pdark-HR from colony 3) at 9pm
on 4th, Sep
induced with darkness at 9pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each)
on 5th, Sep
cultivated with white light at 9am, then took the second 6 samples,
took the third 6 samples at 10am
took the fourth 6 samples at 11am
took the fifth 6 samples at 1pm
took the sixth 6 samples at 9pm
on 6th, Sep
took the seventh 6 samples at 9am
took the eighth 6 samples at 9pm