Difference between revisions of "Template:SJTU-BioX-Shanghai/Notebook/Transformation/w9"
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====second desalination test of pdark-HR==== | ====second desalination test of pdark-HR==== | ||
| − | =====Sep 2===== | + | =====Sep. 2===== |
inoculated 24 wildtype & 24 pdark-HR (use pdark-HR from colony 3) at 9pm | inoculated 24 wildtype & 24 pdark-HR (use pdark-HR from colony 3) at 9pm | ||
Revision as of 08:52, 16 September 2015
Contents
Transformation
pdark natural transformation
In the evening of Aug 31
3rd liquid segregation of pdark-HR
In the evening of Sep 3
4th liquid segregation of pdark-HR
In the evening of Sep 6
5th liquid segregation of pdark-HR
pcpcG2 electro-transformation
In the evening of Sep. 2
2nd liquid segregation of pcpcG2-HR
In the evening of Sep. 4
3rd liquid segregation of pcpcG2-HR
In the evening of Sep. 6
4th liquid segregation of pcpcG2-HR
second desalination test of pdark-HR
Sep. 2
inoculated 24 wildtype & 24 pdark-HR (use pdark-HR from colony 3) at 9pm
Sep 4
induced with darkness at 9pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each)
Sep 5
cultivated with white light at 9am, then took the second 6 samples,
took the third 6 samples at 10am
took the fourth 6 samples at 11am
took the fifth 6 samples at 1pm
took the sixth 6 samples at 9pm
Sep 6
took the seventh 6 samples at 9am
took the eighth 6 samples at 9pm